A novel calmodulin-binding protein, belonging to the WD-repeat family, is localized in dendrites of a subset of CNS neurons
Open accessAbstract
A rat brain synaptosomal protein of 110,000 M(r) present in a fraction highly enriched in adenylyl cyclase activity was microsequenced (Castets, F., G. Baillat, S. Mirzoeva, K. Mabrouk, J. Garin, J. d'Alayer, and A. Monneron. 1994. Biochemistry. 33:5063-5069). Peptide sequences were used to clone a cDNA encoding a novel, 780-amino acid protein named striatin. Striatin is a member of the WD-repeat family (Neer, E.J., C.J. Schmidt, R. Nambudripad, and T.F. Smith. 1994. Nature (Lond.). 371:297-300), the first one known to bind calmodulin (CaM) in the presence of Ca++. Subcellular fractionation shows that striatin is a membrane-associated, Lubrol-soluble protein. As analyzed by Northern blots, in situ hybridization, and immunocytochemistry, striatin is localized in the central nervous system, where it is confined to a subset of neurons, many of which are associated with the motor system. In particular, striatin is conspicuous in the dorsal part of the striatum, as well as in motoneurons. Furthermore, striatin is essentially found in dendrites, but not in axons, and is most abundant in dendritic spines. We propose that striatin interacts, through its WD-repeat domain and in a CaM/Ca(++)-dependent manner, with one or several members of a surrounding cluster of molecules engaged in a Ca(++)-signaling pathway specific to excitatory synapses.
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The B′′′ family consists of Striatin, Striatin-3, and Striatin-4, which localize mainly to dendritic spines of the corpus striatum.
“The B”’ family of regulatory subunits is comprised of 3 members: Striatin, Striatin-3 (also known as SG2NA), and Striatin-4 (also known as Zinedin). They are localized primarily in dendritic spines of the corpus striatum, which is an essential component of the motor system (Castets et al., 1996; Moqrich et al., 1998; Salin et al., 1998).”
From PP2A holoenzymes, substrate specificity driving cellular functions and deregulation in cancer (2019) · cites this paper as Castets et al., 1996
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- Authors
- Castets, F., Bartoli, M., Barnier, J. V., Baillat, G., Salin, P., Moqrich, A., Bourgeois, J. P., Denizot, F., Rougon, G., Calothy, G., & Monneron, A.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1996-08-15
- Volume
- 134
- Issue
- 4
- Pages
- 1051-1062
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2120968
- OpenAlex
- W2120497500
- Cited by
- 129
- References
- 51
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 5 · 2023: 3 · 2022: 4 · 2021: 4 · 2020: 3 · 2019: 7 · 2018: 4 · 2017: 5 · 2016: 4 · 2015: 3 · 2014: 12 · 2013: 5 · 2012: 5
- FWCI
- 1.08
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Keywords
- striatin, calmodulin-binding proteins, WD-repeat proteins, dendrites, dendritic spines, dorsal striatum, motor neurons, calcium signaling, excitatory synapses, adenylate cyclase, synaptosomal proteins, central nervous system neurons
- MeSH terms
- Cyclic AMP, Adenylyl Cyclases, Amino Acid Sequence, Animals, Base Sequence, Calmodulin-Binding Proteins, Cell Fractionation, Central Nervous System, Cloning, Molecular, Corpus Striatum, Dendrites, Male, Molecular Sequence Data, Molecular Weight, Motor Neurons, Peptides, RNA, Messenger, Solubility, Rats, Sprague-Dawley, Rats, Wistar, Sequence Analysis, Sequence Analysis, DNA, DNA, Complementary, Rats
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