CoChat
Sign Up
Cited by 142Escherichia coli research studiesBiochemical and Structural CharacterizationCell Adhesion Molecules Research

The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.

The O75X adhesin of uropathogenic Escherichia coli is a type IV collagen‐binding protein

Westerlund, B., Kuusela, P., Risteli, J., Risteli, L., Vartio, T., Rauvala, H., Virkola, R., Korhonen, T. K.·1989·Molecular Microbiology
DOI 10.1111/j.1365-2958.1989.tb00178.x
Open this paper in CoChat — free account

Abstract

Summary: Interaction of the basement‐membrane binding O75X adhesin of uropathogenic Escherichia coli with various extracellular matrix proteins was studied. The adhesin showed strong binding to type IV collagen immobilized on microtitre plates, whereas other collagens, laminin and fibronectin, were only weakly recognized. Similarly, specific binding of [125 I]‐labelled type IV collagen to 075X‐positive bacteria was shown. Interaction of the two proteins was also demonstrated by affinity chromatography of the O75X adhesin on immobilized type IV collagen. The adhesin bound strongly to the immobilized N‐terminal 7S domain of type IV collagen, and the binding of [125 I]‐labelled type IV collagen to O75X‐positive bacteria was inhibited by the soluble 7S domain. Binding of O75X to type IV collagen and to its 7S domain was specifically inhibited by chloramphenicol but was not affected by periodate or endoglycosidase‐H treatment of the glycoproteins. Our results show that the 7S domain of type IV collagen is the basement membrane receptor for the O75X adhesin and suggest an interaction based on protein‐protein recognition. Inhibition of the interaction by chloramphenicol favours the supposition that a modified tyrosine is involved in the binding site.

Overview

Integrity signals

Registry

Crossref · 10.1111/j.1365-2958.1989.tb00178.x

Cites

53 of 53 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Research works printed but not linked: 6

  1. 10.1111/j.1574-6968.1988.tb02781.x · DOI
  2. Källenius G., 1980, The P antigen as receptor for the hemagglutinin of pyelonephritogenic, Escherichia coli FEMS Microbiol Lett, 3, 313
  3. 10.1111/j.1574-6968.1986.tb01549.x · DOI
  4. Kornblith A.R., 1985, Primary structure of human fibronectin: differential slicing may generate at least 10 polypeptides from a single gene, EMBO J, 4, 175
  5. 10.1111/j.1574-6968.1983.tb00555.x · DOI
  6. 10.1111/j.1574-6968.1987.tb02035.x · DOI

Cited by

Sorted by citations

See all 142

Related

See all

Metadata

Authors
Westerlund, B., Kuusela, P., Risteli, J., Risteli, L., Vartio, T., Rauvala, H., Virkola, R., & Korhonen, T. K.
Affiliations
  • University of Oulu (FI)
  • University of Helsinki (FI)
ORCID
Venue
Molecular Microbiology
Publisher
Wiley
Published
1989-03
Volume
3
Issue
3
Pages
329-337
ISSN
  • 0950-382X (ISSN-L)
  • 1365-2958
DOI
10.1111/j.1365-2958.1989.tb00178.x
OpenAlex
W2075419819
Cited by
142
References
53
Citations by year
2026: 2 · 2025: 3 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 2 · 2018: 1 · 2016: 3 · 2015: 1 · 2014: 3 · 2013: 7 · 2012: 4
FWCI
5.68
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 6449 d)
Locations
  • Molecular Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
Keywords
uropathogenic Escherichia coli, type IV collagen, bacterial adhesion, extracellular matrix proteins, laminin, fibronectin, chloramphenicol inhibition, protein-protein interaction
MeSH terms
Acetylglucosaminidase, Bacterial Adhesion, Bacterial Outer Membrane Proteins, Basement Membrane, Chloramphenicol, Chromatography, Affinity, Collagen, Escherichia coli, Fibronectins, Laminin, Periodic Acid, Protein Binding, Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase, Adhesins, Escherichia coli

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review