The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.
The O75X adhesin of uropathogenic Escherichia coli is a type IV collagen‐binding protein
Abstract
Summary: Interaction of the basement‐membrane binding O75X adhesin of uropathogenic Escherichia coli with various extracellular matrix proteins was studied. The adhesin showed strong binding to type IV collagen immobilized on microtitre plates, whereas other collagens, laminin and fibronectin, were only weakly recognized. Similarly, specific binding of [125 I]‐labelled type IV collagen to 075X‐positive bacteria was shown. Interaction of the two proteins was also demonstrated by affinity chromatography of the O75X adhesin on immobilized type IV collagen. The adhesin bound strongly to the immobilized N‐terminal 7S domain of type IV collagen, and the binding of [125 I]‐labelled type IV collagen to O75X‐positive bacteria was inhibited by the soluble 7S domain. Binding of O75X to type IV collagen and to its 7S domain was specifically inhibited by chloramphenicol but was not affected by periodate or endoglycosidase‐H treatment of the glycoproteins. Our results show that the 7S domain of type IV collagen is the basement membrane receptor for the O75X adhesin and suggest an interaction based on protein‐protein recognition. Inhibition of the interaction by chloramphenicol favours the supposition that a modified tyrosine is involved in the binding site.
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“In Escherichia coli, various adhesins from different fimbrial types facilitate binding to host ECM proteins, such as collagens and fibronectin, which contributes to its persistent colonization and infection58–62.”
From Adhesin Als4112 promotes Candida auris skin colonization through interactions with keratinocytes and extracellular matrix proteins (2025) · cites this paper as [59]
Citations
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Self-citation
Research works printed but not linked: 6
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Lähteenmäki, K., Kuusela, P., & Korhonen, T. (2001) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2001.tb00590.x · Cited by 355
Self-citationBouguénec, C. L., Archambaud, M., & Labigne-Roussel, A. (1992) · Journal of Clinical Microbiology · doi:10.1128/jcm.30.5.1189-1193.1992 · Cited by 339
Bacterial proteins binding to the mammalian extracellular matrix
Westerlund, B., & Korhonen, T. K. (1993) · Molecular Microbiology · doi:10.1111/j.1365-2958.1993.tb01729.x · Cited by 332
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Metadata
- Authors
- Westerlund, B., Kuusela, P., Risteli, J., Risteli, L., Vartio, T., Rauvala, H., Virkola, R., & Korhonen, T. K.
- Affiliations
- University of Oulu (FI)
- University of Helsinki (FI)
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 1989-03
- Volume
- 3
- Issue
- 3
- Pages
- 329-337
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W2075419819
- Cited by
- 142
- References
- 53
- Citations by year
- 2026: 2 · 2025: 3 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 2 · 2018: 1 · 2016: 3 · 2015: 1 · 2014: 3 · 2013: 7 · 2012: 4
- FWCI
- 5.68
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Keywords
- uropathogenic Escherichia coli, type IV collagen, bacterial adhesion, extracellular matrix proteins, laminin, fibronectin, chloramphenicol inhibition, protein-protein interaction
- MeSH terms
- Acetylglucosaminidase, Bacterial Adhesion, Bacterial Outer Membrane Proteins, Basement Membrane, Chloramphenicol, Chromatography, Affinity, Collagen, Escherichia coli, Fibronectins, Laminin, Periodic Acid, Protein Binding, Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase, Adhesins, Escherichia coli
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