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Cited by 50Bacterial Genetics and BiotechnologyBiochemical and Structural CharacterizationProbiotics and Fermented Foods

The Lactococcus lactis sex‐factor aggregation gene cluA

Godon, Jean‐Jacques, Jury, Karen, Shearman, Claire A., Gasson, Michael J.·1994·Molecular Microbiology
DOI 10.1111/j.1365-2958.1994.tb01053.x
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Abstract

Summary: A gene, cluA, was cloned from the chromosomally located sex factor of Lactococcus lactis MG1363. Sequence analysis revealed significant homology with previously described aggregation proteins in Enterococcus and Streptococcus species. The possibility that cluA was an equivalent protein involved in cell aggregation between donor and recipient bacteria during lactococcal conjugation was confirmed by its expression under the control of a heterologous promoter in L. lactis. Analysis of the homology between the CluA protein and the related proteins of Enterococcus and Streptococcus allowed a common structure for these proteins to be postulated. This consisted of five domains. Functionally conserved domains I and V act respectively as a secretary leader and C‐terminal membrane anchor. Domains II and IV are conserved at the amino acid level and probably have common structural roles whereas domain III is variable and may control binding specificity.

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Crossref · 10.1111/j.1365-2958.1994.tb01053.x

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  • Concerning L. lactis, three surface proteins are attributed to the CWAP group: the chromosomally-encoded sex factor CluA, the plasmid-encoded proteinase NisP, and the plasmid-encoded cell serine proteinase PrtP.

    “Concerning L. lactis, three surface proteins were attributed to the same group of CWAP: i) the chromosomally-encoded sex factor CluA [22], ii) the plasmid-encoded proteinase NisP [23], and iii) the plasmid-encoded cell serine proteinase PrtP (also called lactocepin [24], which initiates proteolytic degradation of milk casein [25]).”

    From Positive role of cell wall anchored proteinase PrtP in adhesion of lactococci (2007) · cites this paper as [22]

  • Two confirmed lactococcal proteins with cell wall anchor domains are the sex factor protein CluA and plasmid-encoded NisP, which is not present in MG1363.

    “Two confirmed lactococcal proteins with cell wall anchor domains are the sex factor protein CluA [22], and plasmid-encoded NisP [23], which is not present in MG1363.”

    From Positive role of cell wall anchored proteinase PrtP in adhesion of lactococci (2007) · cites this paper as [22]

  • The CluA dependent cell aggregation phenotype is reportedly poorly expressed unless a co-integrate is formed between the sex factor and a lactose plasmid, so CluA is unlikely to be a significant adhesion factor in the experimental system.

    “The CluA dependent cell aggregation phenotype is reportedly poorly expressed unless a co-integrate is formed between the sex factor and a lactose plasmid [22], so we consider it unlikely that CluA is a significant adhesion factor in our experimental system.”

    From Positive role of cell wall anchored proteinase PrtP in adhesion of lactococci (2007) · cites this paper as [22]

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Authors
Godon, J., Jury, K., Shearman, C. A., & Gasson, M. J.
Affiliations
Norwich Research Park (GB)
Corresponding author
Gasson, Michael J.
ORCID
Godon, Jean‐Jacques
Venue
Molecular Microbiology
Publisher
Wiley
Published
1994-05
Volume
12
Issue
4
Pages
655-663
ISSN
  • 0950-382X (ISSN-L)
  • 1365-2958
DOI
10.1111/j.1365-2958.1994.tb01053.x
OpenAlex
W2168568850
Cited by
50
References
37
Citations by year
2022: 2 · 2020: 1 · 2019: 2 · 2016: 1 · 2014: 3 · 2013: 1 · 2012: 1
FWCI
2.53
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 4562 d)
Locations
  • Molecular Microbiology (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Funders
Institut National de la Recherche Agronomique
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
Keywords
Lactococcus lactis, sex factors, conjugation, cell aggregation, protein domain structure, Enterococcus, Streptococcus, protein secretion, membrane anchor, binding specificity, gene cloning
MeSH terms
Amino Acid Sequence, Bacterial Proteins, Base Sequence, Cloning, Molecular, Conjugation, Genetic, F Factor, Genes, Bacterial, Molecular Sequence Data, Lactococcus lactis, Gene Expression, Sequence Homology, Amino Acid, DNA Primers

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