The DOI matches its Crossref record; 71 of the 71 references it cites were found in a registry or an index.
ExoU is a potent intracellular phospholipase
Open accessAbstract
Summary: The combination of a large genome encoding metabolic versatility and conserved secreted virulence determinants makes Pseudomonas aeruginosa a model pathogen that can be used to study host–parasite interactions in many eukaryotic hosts. One of the virulence regulons that likely plays a role in the ability of P. aeruginosa to avoid innate immune clearance in mammals is a type III secretion system (TTSS). Upon cellular contact, the P. aeruginosa TTSS is capable of delivering a combination of at least four different effector proteins, exoenzyme S (ExoS), ExoT, ExoU, and ExoY. Two of the four translocated proteins, ExoS and ExoU, are cytotoxic to cells during infection and transfection. The mechanism of cytotoxicity of ExoS is unclear. ExoU, however, has recently been characterized as a member of the phospholipase A family of enzymes, possessing at least phospholipase A2 activity. Similar to ExoS, ExoT and ExoY, ExoU requires either a eukaryotic‐specific modification or cofactor for its activity in vitro. The biologic effects of minimal expression of ExoU in yeast can be visualized by membrane damage to different organelles and fragmentation of the vacuole. In mammalian cells, the direct injection of ExoU causes irreversible damage to cellular membranes and rapid necrotic death. ExoU likely represents a unique enzyme and is the first identified phopholipase virulence factor that is translocated into the cytosol by TTSS.
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Cited by
Wiedmaier, N., Müller, S., Köberle, M., Manncke, B., Krejci, J., Autenrieth, I. B., & Bohn, E. (2008) · International Journal of Medical Microbiology · doi:10.1016/j.ijmm.2007.06.001 · Cited by 34
Metadata
- Authors
- Sato, H., & Frank, D. W.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2004-08-03
- Volume
- 53
- Issue
- 5
- Pages
- 1279-1290
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W2140167462
- Cited by
- 287
- References
- 71
- FWCI
- 6.34
- Type
- journal article
- Language
- en
- Open access
- bronze
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
