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Cited by 253

The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.

Genome‐based discovery, structure prediction and functional analysis of cyclic lipopeptide antibiotics in Pseudomonas species

Open access
de Bruijn, Irene, de Kock, Maarten J. D., Yang, Meng, de Waard, Pieter, van Beek, Teris A., Raaijmakers, Jos M.·2007·Molecular Microbiology
DOI 10.1111/j.1365-2958.2006.05525.x
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Abstract

Summary: Analysis of microbial genome sequences have revealed numerous genes involved in antibiotic biosynthesis. In Pseudomonads, several gene clusters encoding non‐ribosomal peptide synthetases (NRPSs) were predicted to be involved in the synthesis of cyclic lipopeptide (CLP) antibiotics. Most of these predictions, however, are untested and the association between genome sequence and biological function of the predicted metabolite is lacking. Here we report the genome‐based identification of previously unknown CLP gene clusters in plant pathogenic Pseudomonas syringae strains B728a and DC3000 and in plant beneficial Pseudomonas fluorescens Pf0‐1 and SBW25. For P. fluorescens SBW25, a model strain in studying bacterial evolution and adaptation, the structure of the CLP with a predicted 9‐amino acid peptide moiety was confirmed by chemical analyses. Mutagenesis confirmed that the three identified NRPS genes are essential for CLP synthesis in strain SBW25. CLP production was shown to play a key role in motility, biofilm formation and in activity of SBW25 against zoospores of Phytophthora infestans. This is the first time that an antimicrobial metabolite is identified from strain SBW25. The results indicate that genome mining may enable the discovery of unknown gene clusters and traits that are highly relevant in the lifestyle of plant beneficial and plant pathogenic bacteria.

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Metadata

Authors
de Bruijn, I., de Kock, M. J. D., Yang, M., de Waard, P., van Beek, T. A., & Raaijmakers, J. M.
Venue
Molecular Microbiology
Publisher
Wiley
Published
2006-11-21
Volume
63
Issue
2
Pages
417-428
ISSN
  • 0950-382X (ISSN-L)
  • 1365-2958
DOI
10.1111/j.1365-2958.2006.05525.x
OpenAlex
W1838261026
Cited by
253
References
44
FWCI
6.79
Type
journal article
Language
en
Open access
bronze

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de Bruijn, I., de Kock, M. J. D., Yang, M., de Waard, P., van Beek, T. A., & Raaijmakers, J. M. (2007). Genome‐based discovery, structure prediction and functional analysis of cyclic lipopeptide antibiotics in Pseudomonas species. Molecular Microbiology. https://doi.org/10.1111/j.1365-2958.2006.05525.x

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.