The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Biosynthesis and IroC‐dependent export of the siderophore salmochelin are essential for virulence of Salmonella enterica serovar Typhimurium
Abstract
In response to iron deprivation, Salmonella enterica serovar Typhimurium secretes two catecholate-type siderophores, enterobactin and its glucosylated derivative salmochelin. Although the systems responsible for enterobactin synthesis and acquisition are well characterized, the mechanisms of salmochelin secretion and acquisition, as well as its role in Salmonella virulence, are incompletely understood. Herein we show by liquid chromatography-mass spectrometry analysis of culture supernatants from wild type and isogenic mutant bacterial strains that the Major Facilitator Superfamily pump EntS is the major exporter of enterobactin and the ABC transporter IroC exports both salmochelin and enterobactin. Growth promotion experiments demonstrate that IroC is not required for utilization of Fe-enterobactin or Fe-salmochelin, as had been previously suggested, but the ABC transporter protein FepD is required for utilization of both siderophores. Salmonella mutants deficient in salmochelin synthesis or secretion exhibit reduced virulence during systemic infection of mice.
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In the citing paper
“The activation and expression of such microbial iron acquisition systems is linked to their pathogenicity and proliferation (Rabsch et al., 1999; Schrettl et al., 2004; Crouch et al., 2008; Andrews-Polymenis et al., 2010; Cassat and Skaar, 2013).”
From Iron at the interface of immunity and infection (2014) · cites this paper as Crouch et al., 2008
In the citing paper
“we infected macrophages with a triple sit feo entC mutant strain of S.Tm defective for the three main iron acquisition pathways (Crouch et al, 2008).”
From Iron Regulatory Proteins Mediate Host Resistance to Salmonella Infection (2015) · cites this paper as Crouch et al, 2008
In the citing paper
“Although, the differences in bacterial counts between ctrl.sh and hmox.sh macrophages were less prominent than during infection with wt Salmonella, intramacrophage numbers of mutant Salmonella were still significantly lower upon hmox silencing than in wt macrophages.”
From Heme oxygenase 1 controls early innate immune response of macrophages to Salmonella Typhimurium infection: Heme oxygenase 1 expression affects Salmonella infection (2016) · cites this paper as Crouch et al., 2008
In the citing paper
“The mutant strain, deficient in enterobactin synthesis, SitABCD- and Feo-mediated iron uptake (ΔentCΔsitΔfeo) was constructed and grown as previously described (Crouch et al., 2008).”
From Heme oxygenase 1 controls early innate immune response of macrophages to Salmonella Typhimurium infection: Heme oxygenase 1 expression affects Salmonella infection (2016) · cites this paper as Crouch et al., 2008
Citations
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Structure, Function, and Evolution of Bacterial ATP-Binding Cassette Systems
Davidson, A. L., Dassa, E., Orelle, C., & Chen, J. (2008) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00031-07 · Cited by 1,477
Probiotic Bacteria Reduce Salmonella Typhimurium Intestinal Colonization by Competing for Iron
Deriu, E., Liu, J. Z., Pezeshki, M., Edwards, R. A., Ochoa, R. J., Contreras, H., Libby, S. J., Fang, F. C., & Raffatellu, M. (2013) · Cell Host & Microbe · doi:10.1016/j.chom.2013.06.007 · Cited by 550
Self-citationRaffatellu, M., George, M. D., Akiyama, Y., Hornsby, M. J., Nuccio, S.-P., Paixao, T. A., Butler, B. P., Chu, H., Santos, R. L., Berger, T., et al. (2009) · Cell Host & Microbe · doi:10.1016/j.chom.2009.03.011 · Cited by 525
Immunometabolism at the interface between macrophages and pathogens
Russell, D. G., Huang, L., & VanderVen, B. C. (2019) · Nature reviews. Immunology · doi:10.1038/s41577-019-0124-9 · Cited by 470
Microbial siderophores: a mini review
Saha, R., Saha, N., Donofrio, R. S., & Bestervelt, L. L. (2012) · Journal of Basic Microbiology · doi:10.1002/jobm.201100552 · Cited by 470
Metadata
- Authors
- Crouch, M. V., Castor, M., Karlinsey, J. E., Kalhorn, T., & Fang, F. C.
- Affiliations
- University of Washington (US)
- Corresponding author
- Fang, Ferric C.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2008-01-14
- Volume
- 67
- Issue
- 5
- Pages
- 971-983
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W2087509245
- Cited by
- 187
- References
- 46
- Citations by year
- 2026: 8 · 2025: 4 · 2024: 6 · 2023: 8 · 2022: 13 · 2021: 10 · 2020: 14 · 2019: 10 · 2018: 6 · 2017: 9 · 2016: 14 · 2015: 12 · 2014: 14 · 2013: 12 · 2012: 11
- FWCI
- 6.33
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases (AI54052, AI 39557, R01AI050660, AI50660, F32AI054052)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Salmonella and Campylobacter epidemiology
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Keywords
- salmochelin, enterobactin, siderophore biosynthesis, iron acquisition, iron starvation, Salmonella typhimurium, bacterial virulence, catecholate siderophores, systemic infection
- MeSH terms
- Animals, Bacterial Proteins, Biological Transport, Chromatography, Liquid, Enterobactin, Iron, Membrane Proteins, Salmonella Infections, Animal, Salmonella typhimurium, Mass Spectrometry, Virulence, Siderophores, Sequence Deletion, ATP-Binding Cassette Transporters, Mice
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