Characterization of the ferrous iron uptake system of Escherichia coli
Open accessAbstract
Escherichia coli has an iron(II) transport system (feo) which may make an important contribution to the iron supply of the cell under anaerobic conditions. Cloning and sequencing of the iron(II) transport genes revealed an open reading frame (feoA) possibly coding for a small protein with 75 amino acids and a membrane protein with 773 amino acids (feoB). The upstream region of feoAB contained a binding site for the regulatory protein Fur, which acts with iron(II) as a corepressor in all known iron transport systems of E. coli. In addition, a Fnr binding site was identified in the promoter region. The FeoB protein had an apparent molecular mass of 70 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and was localized in the cytoplasmic membrane. The sequence revealed regions of homology to ATPases, which indicates that ferrous iron uptake may be ATP driven. FeoA or FeoB mutants could be complemented by clones with the feoA or feoB gene, respectively.
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Alternative ferrous Fe import systems have been identified, including ZupT, YfeABCD, FutABC and EfeUOB.
“To date, alternative ferrous Fe import systems have been identified, including ZupT, YfeABCD, FutABC and EfeUOB 71-76 .”
From Nutritional immunity: the battle for nutrient metals at the host–pathogen interface (2022) · cites this paper as [76]
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- Authors
- Kammler, M., Schön, C., & Hantke, K.
- Affiliations
- University of Tübingen (DE)
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1993-10
- Volume
- 175
- Issue
- 19
- Pages
- 6212-6219
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC206716
- OpenAlex
- W2137147102
- Cited by
- 460
- References
- 2
- Citations by year
- 2026: 7 · 2025: 9 · 2024: 11 · 2023: 12 · 2022: 34 · 2021: 15 · 2020: 12 · 2019: 12 · 2018: 19 · 2017: 11 · 2016: 16 · 2015: 19 · 2014: 20 · 2013: 20 · 2012: 19
- FWCI
- 5.28
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Bacteriology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Keywords
- Escherichia coli, ferrous iron uptake, FeoB, cytoplasmic membrane proteins, gene cloning and sequencing, gene complementation
- MeSH terms
- Adenosine Triphosphatases, Aerobiosis, Amino Acid Sequence, Anaerobiosis, Animals, Base Sequence, Binding Sites, Biological Transport, Cattle, Chromosomes, Bacterial, Escherichia coli, Genes, Bacterial, Genotype, Iron, Kinetics, Molecular Sequence Data, Phenotype, Plasmids, Sequence Homology, Nucleic Acid, Restriction Mapping, Open Reading Frames, Sequence Homology, Amino Acid
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