Dbf2 is essential for cytokinesis and correct mitotic spindle formation in Candida albicans
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Summary: We have characterized the DBF2 gene, encoding a protein kinase of the NDR family in Candida albicans, and demonstrate that this gene is essential for cell viability. Conditional mutants were constructed by using the MET3 promoter to analyse the phenotype of cells lacking this kinase. The absence of Dbf2 resulted in cells arrested as large‐budded pairs that failed to contract the actomyosin ring, a function similar to that described for its Saccharomyces cerevisiae orthologue. In addition to its role in cytokinesis, Dbf2 regulates mitotic spindle organization and nuclear segregation as Dbf2‐depleted cells have abnormal microtubules and severe defects in nuclear migration to the daughter cell, which results in a cell cycle block during mitosis. Taken together, these results imply that Dbf2 performs several functions during exit from mitosis and cytokinesis. Consistent with a role in spindle organization, the protein localizes to the mitotic spindle during anaphase, and it interacts physically with tubulin, as indicated by immunoprecipitation experiments. Finally, DBF2 depletion also resulted in impaired true hyphal growth.
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There are clear phenotypic similarities between morphologies found in this study and C. albicans strains that cannot degrade Clb2, have SIC1 deleted, or have Dbf2 depleted.
“Supporting this connection are clear phenotypic similarities between morphologies found in our studies and C. albicans strains that, for example, cannot degrade Clb2, have the C. albicans homologue of SIC1 deleted, or have Dbf2 levels depleted (Atir-Lande et al., 2005; Bensen et al., 2005; Gonzalez-Novo et al., 2009; Chou et al., 2011).”
From Cdc28 provides a molecular link between Hsp90, morphogenesis, and cell cycle progression in<i>Candida albicans</i> (2012) · cites this paper as Gonzalez-Novo et al., 2009
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- Authors
- González‐Novo, A., Labrador, L., Pablo‐Hernando, M. E., Correa‐Bordes, J., Sánchez, M., Jiménez, J., & De Aldana, C. R. V.
- Affiliations
- Consejo Superior de Investigaciones Científicas (ES)
- Universidad de Salamanca (ES)
- Universidad de Extremadura (ES)
- Universitat Pompeu Fabra (ES)
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2009-06
- Volume
- 72
- Issue
- 6
- Pages
- 1364-1378
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W2082193925
- Cited by
- 22
- References
- 56
- Citations by year
- 2026: 1 · 2024: 1 · 2022: 3 · 2020: 1 · 2019: 2 · 2018: 3 · 2017: 2 · 2016: 2 · 2014: 1
- FWCI
- 0.89
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- Locations
- Molecular Microbiology (publishedVersion)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, other-oa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- NDR kinase, Candida albicans, cytokinesis, mitotic spindle formation, nuclear segregation, microtubules, tubulin, hyphal growth, conditional mutants, cell cycle arrest, mitosis
- MeSH terms
- Actomyosin, Candida albicans, Cell Cycle, Fungal Proteins, Genes, Fungal, Microtubules, Spindle Apparatus, Mutation, Cell Cycle Proteins, Genes, Essential, Hyphae, Cytokinesis
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