High‐resolution boundary analysis during Arabidopsis thaliana flower development
Open accessAbstract
Summary: We report a comparative analysis of cell proliferation patterns during Arabidopsis flower development. Cell division was evaluated by a direct method, i.e. the 5‐bromo‐2′‐deoxyuridine (BrdU) incorporation/immunodetection procedure. BrdU patterns in wild‐type plants were correlated with the expression profiles of both several cell cycle genes involved in the control of the G 1/S transition and cell cycle‐related repressor genes, MSI4 and MSI5, encoding WD‐repeat proteins. To evaluate how proliferation patterns arise with respect to boundaries and vice versa, the expression of a boundary gene, CUP SHAPED COTYLEDON (CUC)2, was determined. Combining these approaches, we demonstrate that boundaries between inflorescence and floral meristems and between floral whorls are narrow bands of non‐dividing cells. In addition, we show that negative and positive regulators of cell proliferation are simultaneously and continuously expressed in dividing meristematic domains, being excluded from boundary cells. Finally, BrdU incorporation and CUC2 in situ hybridisation patterns were analysed in two mutant backgrounds, agamous (ag)‐1 and superman (sup)‐1, in order to assess changes in boundary establishment and different levels of indeterminacy under conditions of altered proliferation at the floral meristem centre.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In Arabidopsis, boundary cells between inflorescence and floral meristems down-regulate DNA synthesis and expression of cell-cycle-related genes.
“In Arabidopsis, boundary cells between inflorescence and floral meristems down-regulate DNA synthesis and expression of cell-cycle-related genes (Breuil-Broyer et al., 2004).”
From <i>Divide et impera</i>: boundaries shape the plant body and initiate new meristems (2016) · cites this paper as Breuil-Broyer et al., 2004
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Genes involved in organ separation in Arabidopsis: an analysis of the cup-shaped cotyledon mutant.
Aida, M., Ishida, T., Fukaki, H., Fujisawa, H., & Tasaka, M. (1997) · The Plant Cell · doi:10.1105/tpc.9.6.841 · Cited by 1,486 · see citing works
Souer, E., van Houwelingen, A., Kloos, D., Mol, J., & Koes, R. (1996) · Cell · doi:10.1016/s0092-8674(00)81093-4 · Cited by 1,059 · see citing works
Flagged first, then by citations
Research works printed but not linked: 3
- Bereterbide, A., Hernould, M., Castera, S. and Mouras, A. (2001) Inhibition of cell proliferation, cell expansion and differentiation ß Blackwell Publishing Ltd, The Plant Journal, (2004), 38, 182±192 by the Arabidopsis SUPERMAN gene in transgenic tobacco plants. Planta, 214, 22±29.
- Doonan, J. (2000) Social controls on cell proliferation in plants [In Process Citation]. Curr. Opin. Plant Biol. 3, 482±487.
- ß Blackwell Publishing Ltd, The Plant Journal, (2004), 38, 182±192 Cell proliferation patterns during flower development 191
Cited by
Sorted by citations
Borghi, L., Bureau, M., & Simon, R. (2007) · The Plant Cell · doi:10.1105/tpc.106.047159 · Cited by 161 · see citing works
Two-Step Regulation of a Meristematic Cell Population Acting in Shoot Branching in Arabidopsis
Shi, B., Zhang, C., Tian, C., Wang, J., Wang, Q., Xu, T., Xu, Y., Ohno, C., Sablowski, R., Heisler, M. G., et al. (2016) · PLoS Genetics · doi:10.1371/journal.pgen.1006168 · Cited by 150 · see citing works
“Because boundary cells are non-dividing or slow-dividing cells [54], leaf axil cells can maintain an undifferentiated status while their neighboring differentiated cells cannot.”
Background<i>Divide et impera</i>: boundaries shape the plant body and initiate new meristems
Wang, Q., Hasson, A., Rossmann, S., & Theres, K. (2016) · New Phytologist · doi:10.1111/nph.13641 · Cited by 106 · see citing works
- Authors
- Breuil‐Broyer, S., Morel, P., De Almeida‐Engler, J., Coustham, V., Negrutiu, I., & Trehin, C.
- Affiliations
- École Normale Supérieure de Lyon (FR)
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 2004-02-18
- Volume
- 38
- Issue
- 1
- Pages
- 182-192
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W2095966125
- Cited by
- 128
- References
- 2
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 3 · 2023: 3 · 2022: 4 · 2021: 4 · 2020: 2 · 2019: 3 · 2018: 7 · 2017: 8 · 2016: 4 · 2015: 9 · 2014: 3 · 2013: 8 · 2012: 3
- FWCI
- 14.10
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Plant Journal (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Flowering Plant Growth and Cultivation
- Keywords
- flower development, cell proliferation, cell division, cell cycle genes, G1/S transition, WD-repeat proteins, boundary formation, floral meristem, inflorescence meristem, AGAMOUS, Superman, Arabidopsis thaliana
- MeSH terms
- Bromodeoxyuridine, Cell Cycle, Cell Division, Mutation, Genes, Plant, Arabidopsis, Gene Expression Regulation, Plant, Gene Expression Regulation, Developmental, Meristem, Arabidopsis Proteins, Flowers
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
