Arabidopsis CUL3A and CUL3B genes are essential for normal embryogenesis
Abstract
Summary: Cullin (CUL)‐dependent ubiquitin ligases form a class of structurally related multisubunit enzymes that control the rapid and selective degradation of important regulatory proteins involved in cell cycle progression and development, among others. The CUL3‐BTB ligases belong to this class of enzymes and despite recent findings on their molecular composition, our knowledge on their functions and substrates remains still very limited. In contrast to budding and fission yeast, CUL3 is an essential gene in metazoans. The model plant Arabidopsis thaliana encodes two related CUL3 genes, called CUL3A and CUL3B . We recently reported that cul3a loss‐of‐function mutants are viable but exhibit a mild flowering and light sensitivity phenotype. We investigated the spatial and temporal expression of the two CUL3 genes in reproductive tissues and found that their expression patterns are largely overlapping suggesting possible functional redundancy. Thus, we investigated the consequences on plant development of combined Arabidopsis cul3a cul3b loss‐of‐function mutations. Homozygous cul3b mutant plants developed normally and were fully fertile. However, the disruption of both the CUL3A and CUL3B genes reduced gametophytic transmission and caused embryo lethality. The observed embryo abortion was found to be under maternal control. Arrest of embryogenesis occurred at multiple stages of embryo development, but predominantly at the heart stage. At the cytological level, CUL3 loss‐of‐function mutations affected both embryo pattern formation and endosperm development.
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CUL3 is required for blue light-induced ubiquitination of PHOT1, and PHOT1 ubiquitination is thought to be involved in phototropism.
“CUL3 is required for blue light-induced ubiquitination of PHOT1, and PHOT1 ubiquitination is thought to be involved in phototropism (Salomon et al., 1997; Thomann et al., 2005; Roberts et al., 2011).”
From The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants (2022) · cites this paper as Thomann et al., 2005
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Genschik, P., Sumara, I., & Lechner, E. (2013) · The EMBO Journal · doi:10.1038/emboj.2013.173 · Cited by 278 · see citing works
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Self-citationThe action of enhancing weak light capture via phototropic growth and chloroplast movement in plants
Xin, G.-Y., Li, L.-P., Wang, P.-T., Li, X.-Y., Han, Y.-J., & Zhao, X. (2022) · Stress Biology · doi:10.1007/s44154-022-00066-x · Cited by 17 · see citing works
“CUL3 is required for blue light-induced ubiquitination of PHOT1, and PHOT1 ubiquitination is thought to be involved in phototropism (Salomon et al., 1997; Thomann et al., 2005; Roberts et al., 2011).”
Background
- Authors
- Thomann, A., Brukhin, V., Dieterle, M., Gheyeselinck, J., Vantard, M., Grossniklaus, U., & Genschik, P.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- University of Zurich (CH)
- Corresponding author
- Genschik, Pascal
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 2005-07-05
- Volume
- 43
- Issue
- 3
- Pages
- 437-448
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W1960125973
- Cited by
- 65
- References
- 53
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 1 · 2020: 3 · 2019: 3 · 2018: 5 · 2017: 6 · 2016: 2 · 2015: 3 · 2014: 2 · 2013: 2 · 2012: 5
- FWCI
- 4.01
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Plant Journal (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant Reproductive Biology
- Keywords
- Arabidopsis thaliana, embryonic development, embryonic lethality, maternal control, endosperm development, embryonic patterning, gene redundancy, loss-of-function mutants
- MeSH terms
- Amino Acid Sequence, Carrier Proteins, Cell Cycle, DNA, Bacterial, Molecular Sequence Data, Mutation, Phenotype, Promoter Regions, Genetic, Seeds, Arabidopsis, Sequence Homology, Amino Acid, Gene Expression Regulation, Plant, Gene Expression Regulation, Developmental, Germination, Gene Expression Profiling, Arabidopsis Proteins, Cullin Proteins
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