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Cited by 262

The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.

Steady‐state transposon mutagenesis in inbred maize

Open access
McCarty, Donald R., Mark Settles, Andrew, Suzuki, Masaharu, Tan, Bao Cai, Latshaw, Susan, Porch, Tim, Robin, Kevin, Baier, John, Avigne, Wayne, Lai, Jinsheng, Messing, Joachim, Koch, Karen E., Curtis Hannah, L.·2005·The Plant Journal
DOI 10.1111/j.1365-313x.2005.02509.x
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Abstract

Summary: We implement a novel strategy for harnessing the power of high‐copy transposons for functional analysis of the maize genome, and report behavioral features of the Mutator system in a uniform inbred background. The unique UniformMu population and database facilitate high‐throughput molecular analysis of Mu‐tagged mutants and gene knockouts. Key features of the population include: (i) high mutation frequencies (7% independent seed mutations) and moderation of copy number (approximately 57 total Mu elements; 1–2 MuDR copies per plant) were maintained by continuous back‐crossing into a phenotypically uniform inbred background; (ii) a bz1‐mum9 marker enabled selection of stable lines (loss of MuDR), inhibiting further transpositions in lines selected for molecular analysis; (iii) build‐up of mutation load was prevented by screening Mu‐active parents to exclude plants carrying pre‐existing seed mutations. To create a database of genomic sequences flanking Mu insertions, selected mutant lines were analyzed by sequencing of MuTAIL PCR clone libraries. These sequences were annotated and clustered to facilitate bioinformatic subtraction of ancestral elements and identification of insertions unique to mutant lines. New insertions targeted low‐copy, gene‐rich sequences, and in silico mapping revealed a random distribution of insertions over the genome. Our results indicate that Mu populations differ markedly in the occurrence of Mu insertion hotspots and the frequency of suppressible mutations. We suggest that controlled MuDR copy number in UniformMu lines is a key determinant of these differences. The public database (http://uniformmu.org; http://endosperm.info) includes pedigree and phenotypic data for over 2000 independent seed mutants selected from a population of 31 548 F2 lines and integrated with analyses of 34 255 MuTAIL sequences.

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Crossref · 10.1111/j.1365-313x.2005.02509.x

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Metadata

Authors
McCarty, D. R., Mark Settles, A., Suzuki, M., Tan, B. C., Latshaw, S., Porch, T., Robin, K., Baier, J., Avigne, W., Lai, J., Messing, J., Koch, K. E., & Curtis Hannah, L.
Venue
The Plant Journal
Publisher
Wiley
Published
2005-09-06
Volume
44
Issue
1
Pages
52-61
ISSN
  • 0960-7412 (ISSN-L)
  • 1365-313X
DOI
10.1111/j.1365-313x.2005.02509.x
OpenAlex
W2101641832
Cited by
262
References
23
FWCI
7.65
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Steady‐state transposon mutagenesis in inbred maize. https://app.cochat.ai/papers/10.1111/j.1365-313x.2005.02509.x
The paper
McCarty, D. R., Mark Settles, A., Suzuki, M., Tan, B. C., Latshaw, S., Porch, T., Robin, K., Baier, J., Avigne, W., Lai, J., Messing, J., Koch, K. E., & Curtis Hannah, L. (2005). Steady‐state transposon mutagenesis in inbred maize. The Plant Journal. https://doi.org/10.1111/j.1365-313x.2005.02509.x

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

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