Interplay of miR164, CUP‐SHAPED COTYLEDON genes and LATERAL SUPPRESSOR controls axillary meristem formation in Arabidopsis thaliana
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Summary: Aerial architecture in higher plants is established post‐embryonically by the inception of new meristems in the axils of leaves. These axillary meristems develop into side shoots or flowers. In Arabidopsis, the NAC domain transcription factors CUP SHAPED COTYLEDON1 (CUC1), CUC2 and CUC3 function redundantly in initiating the shoot apical meristem and establishing organ boundaries. Transcripts of CUC1 and CUC2 are targeted for degradation by miR164. In this study, we show that cuc3‐2 mutants are impaired in axillary meristem initiation. Overexpression of miR164 in the cuc3‐2 mutant caused an almost complete block of axillary meristem formation. Conversely, mir164 mutants and plants harbouring miR164‐resistant alleles of CUC1 or CUC2 developed accessory buds in leaf axils. Collectively, these experiments reveal that, in addition to CUC3, redundant functions of CUC1 and CUC2 as well as miR164 regulation are required for the establishment of axillary meristems. Studies on LAS transcript accumulation in mir164 triple mutants and cuc3‐2 plants overexpressing miR164 suggest that regulation of axillary meristem formation by miR164 is mediated through CUC1 and CUC2, which in turn regulate LAS.
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NAM/CUC genes play a very important role during postembryonic development, as loss of these gene functions leads to defects in shoot branching in Arabidopsis, snapdragon and tomato.
“Beside their involvement in SAM formation, NAM/CUC genes also play a very important role during postembryonic development, as loss of these gene functions leads to defects in shoot branching in Arabidopsis, snapdragon and tomato (Weir et al., 2004;Hibara , 2006;Raman et al., 2008;Busch et al., 2011).”
From <i>Divide et impera</i>: boundaries shape the plant body and initiate new meristems (2016) · cites this paper as Raman et al., 2008
The Results (Figure 1h; page not specified in the indexed passage) show that loss of CUC3 strongly reduces axillary bud formation in Arabidopsis during early and middle vegetative development. The paper does not establish the claimed shoot-branching defects in snapdragon or tomato, so it supports only the Arabidopsis portion of the multi-species statement. (LLM, conf=0.86)
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Cites
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“…development and restored when the plant matures (Mueller et al., 2006). las-4 mutants do not usually develop axillary buds in the vegetative phase, but depending on growth conditions axillary shoots are formed in the axils of the topmost rosette leaves at a low frequency (Greb et al., 2003).”
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“Transcripts of the CUC1, CUC2, CUC3, RAX1 and LAS genes accumulate in overlapping domains in the axils of developing leaf primordia (Greb et al., 2003; Keller et al., 2006; Mueller et al., 2006; Vroemen et al., 2003).”
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<i>CUPULIFORMIS</i> establishes lateral organ boundaries in <i>Antirrhinum</i>
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The NAC-domain transcription factor GOBLET specifies leaflet boundaries in compound tomato leaves
Berger, Y., Harpaz-Saad, S., Brand, A., Melnik, H., Sirding, N., Alvarez, J. P., Zinder, M., Samach, A., Eshed, Y., & Ori, N. (2009) · Development · doi:10.1242/dev.031625 · Cited by 305 · see citing works
Hormonal regulation of branching in grasses
McSteen, P. (2009) · Plant Physiology · doi:10.1104/pp.108.129056 · Cited by 193 · see citing works
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“Axillary bud formation was analyzed after the onset of flowering using a stereomicroscope as previously described (Raman et al., 2008).”
BackgroundMethodSelf-citationTwo-Step Regulation of a Meristematic Cell Population Acting in Shoot Branching in Arabidopsis
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“Genetic and molecular studies revealed direct and indirect interactions among these genes in a regulatory network [18, 21].”
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<i>Divide et impera</i>: boundaries shape the plant body and initiate new meristems
Wang, Q., Hasson, A., Rossmann, S., & Theres, K. (2016) · New Phytologist · doi:10.1111/nph.13641 · Cited by 106 · see citing works
Self-citationTissue Culture Responsive MicroRNAs in Strawberry
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Tang, C., Han, R., Zhou, Z., Yang, Y., Zhu, M., Xu, T., Wang, A., Li, Z., & Dong, T. (2020) · Journal of Plant Physiology · doi:10.1016/j.jplph.2020.153224 · Cited by 23 · see citing works
- Authors
- Raman, S., Greb, T., Peaucelle, A., Blein, T., Laufs, P., & Theres, K.
- Affiliations
- Max Planck Institute for Plant Breeding Research (DE)
- Gregor Mendel Institute of Molecular Plant Biology (AT)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Corresponding author
- Theres, Klaus
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 2008-06-28
- Volume
- 55
- Issue
- 1
- Pages
- 65-76
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W2093117995
- Cited by
- 299
- References
- 6
- Citations by year
- 2026: 10 · 2025: 23 · 2024: 17 · 2023: 17 · 2022: 23 · 2021: 24 · 2020: 21 · 2019: 16 · 2018: 13 · 2017: 21 · 2016: 8 · 2015: 19 · 2014: 9 · 2013: 14 · 2012: 21
- FWCI
- 17.34
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Plant Journal (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Digital Library (submittedVersion, other-oa)
- HAL (submittedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion, other-oa)
- Funders
- Deutsche Forschungsgemeinschaft (SFB 572)
- Universität zu Köln
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Keywords
- miR164, CUC1, CUC2, axillary meristem initiation, NAC transcription factors, microRNA regulation, shoot apical meristem, organ boundaries, Arabidopsis thaliana
- MeSH terms
- Multigene Family, Transcription Factors, Arabidopsis, Meristem, Arabidopsis Proteins, MicroRNAs
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