The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii
Open accessAbstract
Summary: MicroRNAs (miRNAs) are small RNAs, 21 to 22 nucleotides long, with important regulatory roles. They are processed from longer RNA molecules with imperfectly matched foldback regions and they function in modulating the stability and translation of mRNA. Recently, we and others have demonstrated that the unicellular alga Chlamydomonas reinhardtii, like diverse multicellular organisms, contains miRNAs. These RNAs resemble the miRNAs of land plants in that they direct site‐specific cleavage of target mRNA with miRNA‐complementary motifs and, presumably, act as regulatory molecules in growth and development. Utilizing these findings we have developed a novel artificial miRNA system based on ligation of DNA oligonucleotides that can be used for specific high‐throughput gene silencing in green algae.
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Genetic Engineering of Algae for Enhanced Biofuel Production
Radakovits, R., Jinkerson, R. E., Darzins, A., & Posewitz, M. C. (2010) · Eukaryotic Cell · doi:10.1128/ec.00364-09 · Cited by 1,142
CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii
Shin, S.-E., Lim, J.-M., Koh, H. G., Kim, E. K., Kang, N. K., Jeon, S., Kwon, S., Shin, W.-S., Lee, B., Hwangbo, K., et al. (2016) · Scientific Reports · doi:10.1038/srep27810 · Cited by 423
Potential of industrial biotechnology with cyanobacteria and eukaryotic microalgae
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Li, X., Zhang, R., Patena, W., Gang, S. S., Blum, S. R., Ivanova, N., Yue, R., Robertson, J. M., Lefebvre, P. A., Fitz-Gibbon, S. T., et al. (2016) · The Plant Cell · doi:10.1105/tpc.15.00465 · Cited by 355
Metadata
- Authors
- Molnar, A., Bassett, A., Thuenemann, E., Schwach, F., Karkare, S., Ossowski, S., Weigel, D., & Baulcombe, D.
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 2009-03-26
- Volume
- 58
- Issue
- 1
- Pages
- 165-174
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W1822279124
- Cited by
- 345
- References
- 48
- FWCI
- 20.82
- Type
- journal article
- Language
- en
- Open access
- bronze
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- The paper
- Molnar, A., Bassett, A., Thuenemann, E., Schwach, F., Karkare, S., Ossowski, S., Weigel, D., & Baulcombe, D. (2009). Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii. The Plant Journal. https://doi.org/10.1111/j.1365-313x.2008.03767.x
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