CoChat
Sign Up
Cited by 285

The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

ATG8 lipidation and ATG8-mediated autophagy in Arabidopsis require ATG12 expressed from the differentially controlled ATG12A AND ATG12B loci: Arabidopsis autophagy requires ATG12

Chung, Taijoon, Phillips, Allison R., Vierstra, Richard D.·2010·The Plant Journal
DOI 10.1111/j.1365-313x.2010.04166.x
Open this paper in CoChat — free account

Abstract

Autophagic recycling of intracellular plant constituents is maintained at a basal level under normal growth conditions but can be induced in response to nutritional demand, biotic stress, and senescence. One route requires the ubiquitin-fold proteins Autophagy-related (ATG)-8 and ATG12, which become attached to the lipid phosphatidylethanolamine (PE) and the ATG5 protein, respectively, during formation of the engulfing vesicle and delivery of its cargo to the vacuole for breakdown. Here, we genetically analyzed the conjugation machinery required for ATG8/12 modification in Arabidopsis thaliana with a focus on the two loci encoding ATG12. Whereas single atg12a and atg12b mutants lack phenotypic consequences, atg12a atg12b double mutants senesce prematurely, are hypersensitive to nitrogen and fixed carbon starvation, and fail to accumulate autophagic bodies in the vacuole. By combining mutants eliminating ATG12a/b, ATG5, or the ATG10 E2 required for their condensation with a method that unequivocally detects the ATG8-PE adduct, we also show that ATG8 lipidation requires the ATG12-ATG5 conjugate. Unlike ATG8, ATG12 does not associate with autophagic bodies, implying that its role(s) during autophagy is restricted to events before the vacuolar deposition of vesicles. The expression patterns of the ATG12a and ATG12b genes and the effects of single atg12a and atg12b mutants on forming the ATG12-ATG5 conjugate reveal that the ATG12b locus is more important during basal autophagy whi…

Overview

Integrity signals

Registry

Crossref · 10.1111/j.1365-313x.2010.04166.x

Cites

3 of 3 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Show all 3 references

Research works printed but not linked: 42

  1. Arnon, 1949, Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris, Plant Physiol., 24, 1, 10.1104/pp.24.1.1 · DOI
  2. Bassham, 2007, Plant autophagy-more than a starvation response, Curr. Opin. Plant Biol., 10, 587, 10.1016/j.pbi.2007.06.006 · DOI
  3. Bassham, 2006, Autophagy in development and stress responses of plants, Autophagy, 2, 2, 10.4161/auto.2092 · DOI
  4. Chung, 2009, The ATG autophagic conjugation system in maize: ATG transcripts and abundance of the ATG8-lipid adduct are regulated by development and nutrient availability, Plant Physiol., 149, 220, 10.1104/pp.108.126714 · DOI · Paper page
  5. Clough, 1998, Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana, Plant J., 16, 735, 10.1046/j.1365-313x.1998.00343.x · DOI
  6. Contento, 2004, Transcriptome profiling of the response of Arabidopsis suspension culture cells to Suc starvation, Plant Physiol., 135, 2330, 10.1104/pp.104.044362 · DOI
  7. Curtis, 2003, A gateway cloning vector set for high-throughput functional analysis of genes in planta, Plant Physiol., 133, 462, 10.1104/pp.103.027979 · DOI
  8. Dice, 2007, Chaperone-mediated autophagy, Autophagy, 3, 295, 10.4161/auto.4144 · DOI
  9. Doelling, 2002, The APG8/12-activating enzyme APG7 is required for proper nutrient recycling and senescence in Arabidopsis thaliana, J. Biol. Chem., 277, 33105, 10.1074/jbc.M204630200 · DOI
  10. Downes, 2005, Post-translational regulation in plants employing a diverse set of polypeptide tags, Biochem. Soc. Trans., 33, 393, 10.1042/BST0330393 · DOI
  11. Forde, 2002, Local and long-range signaling pathways regulating plant responses to nitrate, Annu. Rev. Plant Biol., 53, 203, 10.1146/annurev.arplant.53.100301.135256 · DOI
  12. Fujiki, 2007, An Arabidopsis homolog of yeast ATG6/VPS30 is essential for pollen germination, Plant Physiol., 143, 1132, 10.1104/pp.106.093864 · DOI
  13. Fujioka, 2008, In vitro reconstitution of plant ATG8 and ATG12 conjugation systems essential for autophagy, J. Biol. Chem., 283, 1921, 10.1074/jbc.M706214200 · DOI
  14. Fujita, 2008, The Atg16L complex specifies the site of LC3 lipidation for membrane biogenesis in autophagy, Mol. Biol. Cell, 19, 2092, 10.1091/mbc.e07-12-1257 · DOI
  15. Hanaoka, 2002, Leaf senescence and starvation-induced chlorosis are accelerated by the disruption of an Arabidopsis autophagy gene, Plant Physiol., 129, 1181, 10.1104/pp.011024 · DOI
  16. Harrison-Lowe, 2008, Autophagy protein 6 (ATG6) is required for pollen germination in Arabidopsis thaliana, Autophagy, 4, 339, 10.4161/auto.5629 · DOI
  17. Hofius, 2009, Autophagic components contribute to hypersensitive cell death in Arabidopsis, Cell, 137, 773, 10.1016/j.cell.2009.02.036 · DOI
  18. Hörtensteiner, 2002, Nitrogen metabolism and remobilization during senescence, J. Exp. Bot., 53, 927, 10.1093/jexbot/53.370.927 · DOI
  19. Ichimura, 2000, A ubiquitin-like system mediates protein lipidation, Nature, 408, 488, 10.1038/35044114 · DOI
  20. Kim, 2001, Membrane recruitment of Aut7p in the autophagy and cytoplasm to vacuole targeting pathways requires Aut1p, Aut2p, and the autophagy conjugation complex, J. Cell Biol., 152, 51, 10.1083/jcb.152.1.51 · DOI
  21. Klionsky, 2007, Autophagy: from phenomenology to molecular understanding in less than a decade, Nat. Rev. Mol. Cell Biol., 8, 931, 10.1038/nrm2245 · DOI
  22. Liu, 2005, Autophagy regulates programmed cell death during the plant innate immune response, Cell, 121, 567, 10.1016/j.cell.2005.03.007 · DOI
  23. Melendez, 2003, Autophagy genes are essential for dauer development and life-span extension in C. elegans, Science, 301, 1387, 10.1126/science.1087782 · DOI · Paper page
  24. Mizushima, 1998, A protein conjugation system essential for autophagy, Nature, 395, 395, 10.1038/26506 · DOI
  25. Nishida, 2009, Discovery of Atg5/Atg7-independent alternative macroautophagy, Nature, 461, 654, 10.1038/nature08455 · DOI · Paper page
  26. Osuna, 2007, Temporal responses of transcripts, enzyme activities and metabolites after adding sucrose to carbon-deprived Arabidopsis seedlings, Plant J., 49, 463, 10.1111/j.1365-313X.2006.02979.x · DOI
  27. Peng, 2007, Genome-wide analysis of Arabidopsis responsive transcriptome to nitrogen limitation and its regulation by the ubiquitin ligase gene NLA, Plant Mol. Biol., 65, 775, 10.1007/s11103-007-9241-0 · DOI
  28. Phillips, 2008, The ATG12-conjugating enzyme ATG10 is essential for autophagic vesicle formation in Arabidopsis thaliana, Genetics, 178, 1339, 10.1534/genetics.107.086199 · DOI
  29. Qin, 2007, Arabidopsis AtBECLIN 1/AtAtg6/AtVps30 is essential for pollen germination and plant development, Cell Res., 17, 249, 10.1038/cr.2007.7 · DOI
  30. Qu, 2007, Autophagy gene-dependent clearance of apoptotic cells during embryonic development, Cell, 128, 931, 10.1016/j.cell.2006.12.044 · DOI
  31. Rose, 2006, Starvation-induced expression of autophagy-related genes in Arabidopsis, Biol. Cell, 98, 53, 10.1042/BC20040516 · DOI
  32. Rubinsztein, 2009, In search of an “autophagomometer”, Autophagy, 5, 585, 10.4161/auto.5.5.8823 · DOI · Paper page
  33. Sláviková, 2005, The autophagy-associated ATG8 gene family operates both under favourable growth conditions and under starvation stresses in Arabidopsis plants, J. Exp. Bot., 56, 2839, 10.1093/jxb/eri276 · DOI
  34. Suzuki, 2005, The crystal structure of plant ATG12 and its biological implication in autophagy, Autophagy, 1, 119, 10.4161/auto.1.2.1859 · DOI
  35. Thompson, 2005, Autophagic recycling: lessons from yeast help define the process in plants, Curr. Opin. Plant Biol., 8, 165, 10.1016/j.pbi.2005.01.013 · DOI
  36. Thompson, 2005, Autophagic nutrient recycling in Arabidopsis directed by the ATG8 and ATG12 conjugation pathways, Plant Physiol., 138, 2097, 10.1104/pp.105.060673 · DOI · Paper page
  37. Tsukamoto, 2008, Autophagy is essential for preimplantation development of mouse embryos, Science, 321, 117, 10.1126/science.1154822 · DOI
  38. Van der Graaff, 2006, Transcription analysis of Arabidopsis membrane transporters and hormone pathways during developmental and induced leaf senescence, Plant Physiol., 141, 776, 10.1104/pp.106.079293 · DOI
  39. Xiong, 2005, AtATG18a is required for the formation of autophagosomes during nutrient stress and senescence in Arabidopsis thaliana, Plant J., 42, 535, 10.1111/j.1365-313X.2005.02397.x · DOI
  40. Yoshimoto, 2004, Processing of ATG8s, ubiquitin-like proteins, and their deconjugation by ATG4s are essential for plant autophagy, Plant Cell, 16, 2967, 10.1105/tpc.104.025395 · DOI · Paper page
  41. Yoshimoto, 2009, Autophagy negatively regulates cell death by controlling NPR1-dependent salicylic acid signaling during senescence and the innate immune response in Arabidopsis, Plant Cell, 21, 2914, 10.1105/tpc.109.068635 · DOI
  42. Zhang, 2009, SEPA-1 mediates the specific recognition and degradation of P granule components by autophagy in C. elegans, Cell, 136, 308, 10.1016/j.cell.2008.12.022 · DOI

Cited by

Sorted by citations

See all 285

Metadata

Authors
Chung, T., Phillips, A. R., & Vierstra, R. D.
Venue
The Plant Journal
Publisher
Wiley
Published
2010-02-03
Volume
62
Issue
3
Pages
483-493
ISSN
  • 0960-7412
  • 1365-313X
DOI
10.1111/j.1365-313x.2010.04166.x
Cited by
285
References
3
Language
en
Licence
doi.wiley.com/10.1002/tdm_license_1.1 (tdm, 2036 d)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review