The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Auxin stimulates DWARF4 expression and brassinosteroid biosynthesis in Arabidopsis
Abstract
Summary: Brassinosteroids (BRs) are growth‐promoting steroidal hormones. Despite the importance of BRs in plant biology, the signal that initiates BR biosynthesis remains unknown. Among the enzymes involved in BR biosynthesis in Arabidopsis (Arabidopsis thaliana), DWARF4 catalyzes the rate‐determining step. Through both the histochemical analysis of DWF4pro:GUS plants and the direct measurement of endogenous BR content, we discovered that BR biosynthesis is stimulated by auxin. When DWF4pro:GUS was subjected to auxin dose–response tests and a time‐course analysis, GUS activity started to increase at an auxin concentration of 10 n m, rising noticeably after 1 h of auxin treatment. In addition, the analysis of the DWF4pro:GUS line in BR‐ and auxin‐mutant backgrounds revealed that the induction by auxin requires auxin‐signaling pathways but not BRs, which implies that auxin signaling directly controls BR biosynthesis. Furthermore, chromatin immunoprecipitation assays confirmed that auxin inhibits the binding of the transcriptional repressor, BZR1, to the DWF4 promoter. A microarray analysis that was designed to examine the transcriptomes after treatment with auxin alone or auxin plus brassinazole (a BR biosynthetic inhibitor) revealed that genes previously characterized as being auxin responsive are not properly regulated when BR biosynthesis is disrupted by brassinazole. Therefore, our results support the idea that auxin regulates BR biosynthesis, and that auxin thus relies on synthesized BRs for some of its growth‐promoting effects in Arabidopsis.
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Cites
The F-box protein TIR1 is an auxin receptor
Dharmasiri, N., Dharmasiri, S., & Estelle, M. (2005) · Nature · doi:10.1038/nature03543 · Cited by 2,083
BRASSINOSTEROIDS: Essential Regulators of Plant Growth and Development
Clouse, S. D., & Sasse, J. M. (1998) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.arplant.49.1.427 · Cited by 1,462
Auxin-responsive gene expression: genes, promoters and regulatory factors
Hagen, G., & Guilfoyle, T. (2002) · Plant Molecular Biology · doi:10.1023/a:1015207114117 · Cited by 1,262
Tatematsu, K., Kumagai, S., Muto, H., Sato, A., Watahiki, M. K., Harper, R. M., Liscum, E., & Yamamoto, K. T. (2004) · The Plant Cell · doi:10.1105/tpc.018630 · Cited by 431
Cited by
Brassinosteroid Signaling Network and Regulation of Photomorphogenesis
Wang, Z.-Y., Bai, M.-Y., Oh, E., & Zhu, J.-Y. (2012) · Annual Review of Genetics · doi:10.1146/annurev-genet-102209-163450 · Cited by 490
Zhu, J.-Y., Sae-Seaw, J., & Wang, Z.-Y. (2013) · Development · doi:10.1242/dev.060590 · Cited by 336
Brassinosteroids Regulate Root Growth, Development, and Symbiosis
Wei, Z., & Li, J. (2016) · Molecular Plant · doi:10.1016/j.molp.2015.12.003 · Cited by 323
Brassinosteroids regulate organ boundary formation in the shoot apical meristem of Arabidopsis
Gendron, J. M., Liu, J.-S., Fan, M., Bai, M.-Y., Wenkel, S., Springer, P. S., Barton, M. K., & Wang, Z.-Y. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1210799110 · Cited by 205
Ahanger, M. A., Ashraf, M., Bajguz, A., & Ahmad, P. (2018) · Journal of Plant Growth Regulation · doi:10.1007/s00344-018-9855-2 · Cited by 142
Metadata
- Authors
- Chung, Y., Maharjan, P. M., Lee, O., Fujioka, S., Jang, S., Kim, B., Takatsuto, S., Tsujimoto, M., Kim, H., Cho, S., Park, T., Cho, H., Hwang, I., & Choe, S.
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 2011-04-04
- Volume
- 66
- Issue
- 4
- Pages
- 564-578
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W1596894747
- Cited by
- 166
- References
- 4
- FWCI
- 18.53
- Type
- journal article
- Language
- en
- Open access
- closed
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- Chung, Y., Maharjan, P. M., Lee, O., Fujioka, S., Jang, S., Kim, B., Takatsuto, S., Tsujimoto, M., Kim, H., Cho, S., Park, T., Cho, H., Hwang, I., & Choe, S. (2011). Auxin stimulates DWARF4 expression and brassinosteroid biosynthesis in Arabidopsis. The Plant Journal. https://doi.org/10.1111/j.1365-313x.2011.04513.x
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