The DOI matches its Crossref record; 70 of the 70 references it cites were found in a registry or an index.
A revised mineral nutrient supplement increases biomass and growth rate in Chlamydomonas reinhardtii
Open accessAbstract
Summary: Interest in exploiting algae as a biofuel source and the role of inorganic nutrient deficiency in inducing triacylglyceride (TAG) accumulation in cells necessitates a strategy to efficiently formulate species‐specific culture media that can easily be manipulated. Using the reference organism Chlamydomonas reinhardtii, we tested the hypothesis that modeling trace element supplements after the cellular ionome would result in optimized cell growth. We determined the trace metal content of several commonly used Chlamydomonas strains in various culture conditions and developed a revised trace element solution to parallel these measurements. Comparison of cells growing in the revised supplement versus a traditional trace element solution revealed faster growth rates and higher maximum cell densities with the revised recipe. RNA‐seq analysis of cultures growing in the traditional versus revised medium suggest that the variation in transcriptomes was smaller than that found between different wild‐type strains grown in traditional Hutner’s supplement. Visual observation did not reveal defects in cell motility or mating efficiency in the new supplement. Ni 2+‐inducible expression from the CYC6 promoter remained a useful tool, albeit with an increased requirement for Ni 2+ because of the introduction of an EDTA buffer system in the revised medium. Other advantages include more facile preparation of trace element stock solutions, a reduction in total chemical use, a more consistent batch‐to‐batch formulation and long‐term stability (tested up to 5 years). Under the new growth regime, we analyzed cells growing under different macro‐ and micronutrient deficiencies. TAG accumulation in N deficiency is comparable in the new medium. Fe and Zn deficiency also induced TAG accumulation, as suggested by Nile Red staining. This approach can be used to efficiently optimize culture conditions for other algal species to improve growth and to assay cell physiology.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860
The Chlamydomonas Genome Reveals the Evolution of Key Animal and Plant Functions
Merchant, S. S., Prochnik, S. E., Vallon, O., Harris, E. H., Karpowicz, S. J., Witman, G. B., Terry, A., Salamov, A., Fritz-Laylin, L. K., Maréchal-Drouard, L., et al. (2007) · Science · doi:10.1126/science.1143609 · Cited by 2,691
Self-citationAn outlook on microalgal biofuels
Wijffels, R. H., & Barbosa, M. J. (2010) · Science · doi:10.1126/science.1189003 · Cited by 1,886
The New Higher Level Classification of Eukaryotes with Emphasis on the Taxonomy of Protists
ADL, S. M., SIMPSON, A. G. B., FARMER, M. A., ANDERSEN, R. A., ANDERSON, O. R., BARTA, J. R., BOWSER, S. S., BRUGEROLLE, G., FENSOME, R. A., FREDERICQ, S., et al. (2005) · The Journal of Eukaryotic Microbiology · doi:10.1111/j.1550-7408.2005.00053.x · Cited by 1,744
Gorman, D. S., & Levine, R. P. (1965) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.54.6.1665 · Cited by 1,681
Structural identification of a bacterial quorum-sensing signal containing boron
Chen, X., Schauder, S., Potier, N., Van Dorsselaer, A., Pelczer, I., Bassler, B. L., & Hughson, F. M. (2002) · Nature · doi:10.1038/415545a · Cited by 1,571
Genetic Engineering of Algae for Enhanced Biofuel Production
Radakovits, R., Jinkerson, R. E., Darzins, A., & Posewitz, M. C. (2010) · Eukaryotic Cell · doi:10.1128/ec.00364-09 · Cited by 1,142
(1989) · Elsevier eBooks · doi:10.1016/c2009-0-02778-0 · Cited by 1,128
The Dynamics of Photosynthesis
Eberhard, S., Finazzi, G., & Wollman, F.-A. (2008) · Annual Review of Genetics · doi:10.1146/annurev.genet.42.110807.091452 · Cited by 755
Siaut, M., Cuiné, S., Cagnon, C., Fessler, B., Nguyen, M., Carrier, P., Beyly, A., Beisson, F., Triantaphylidès, C., Li-Beisson, Y., et al. (2011) · BMC Biotechnology · doi:10.1186/1472-6750-11-7 · Cited by 752
Cited by
The lipid biochemistry of eukaryotic algae
Li-Beisson, Y., Thelen, J. J., Fedosejevs, E., & Harwood, J. L. (2019) · Progress in Lipid Research · doi:10.1016/j.plipres.2019.01.003 · Cited by 453
Boyle, N. R., Page, M. D., Liu, B., Blaby, I. K., Casero, D., Kropat, J., Cokus, S. J., Hong-Hermesdorf, A., Shaw, J., Karpowicz, S. J., et al. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.334052 · Cited by 419
Self-citationThe Eukaryotic CO2-Concentrating Organelle Is Liquid-like and Exhibits Dynamic Reorganization
Freeman Rosenzweig, E. S., Xu, B., Kuhn Cuellar, L., Martinez-Sanchez, A., Schaffer, M., Strauss, M., Cartwright, H. N., Ronceray, P., Plitzko, J. M., Förster, F., et al. (2017) · Cell · doi:10.1016/j.cell.2017.08.008 · Cited by 398
Msanne, J., Xu, D., Konda, A. R., Casas-Mollano, J. A., Awada, T., Cahoon, E. B., & Cerutti, H. (2012) · Phytochemistry · doi:10.1016/j.phytochem.2011.12.007 · Cited by 391
Li, X., Zhang, R., Patena, W., Gang, S. S., Blum, S. R., Ivanova, N., Yue, R., Robertson, J. M., Lefebvre, P. A., Fitz-Gibbon, S. T., et al. (2016) · The Plant Cell · doi:10.1105/tpc.15.00465 · Cited by 355
Metadata
- Authors
- Kropat, J., Hong‐Hermesdorf, A., Casero, D., Ent, P., Castruita, M., Pellegrini, M., Merchant, S. S., & Malasarn, D.
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 2011-03-21
- Volume
- 66
- Issue
- 5
- Pages
- 770-780
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W1599232010
- Cited by
- 327
- References
- 70
- FWCI
- 6.30
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A revised mineral nutrient supplement increases biomass and growth rate in Chlamydomonas reinhardtii. https://app.cochat.ai/papers/10.1111/j.1365-313x.2011.04537.x
- The paper
- Kropat, J., Hong‐Hermesdorf, A., Casero, D., Ent, P., Castruita, M., Pellegrini, M., Merchant, S. S., & Malasarn, D. (2011). A revised mineral nutrient supplement increases biomass and growth rate in Chlamydomonas reinhardtii. The Plant Journal. https://doi.org/10.1111/j.1365-313x.2011.04537.x
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
