Uptake and Distribution of Mercury within Higher Plants

Abstract

The uptake and distribution of inorganic mercury (HgCl 2) within higher plants (Pisum sativum and Mentha spicata) was examined using solution culture and radiotracer techniques. Plants were found to tolerate an external level of 1 mgHg/kg of solution but both physiological and biochemical processes were affected at 5 mgHg/kg and 10 mgHg/kg. The uptake of Hg into plants grown in hydroponic solution was a function of external concentration. Over the concentration range considered the accumulation of Hg in the roots was linear on a log‐log basis although the uptake of the element into the shoots appeared to be two‐phased. The distribution of Hg in plants was asymmetrical with much greater amounts of the element in the roots than the shoots. Although the level of Hg increased generally in plant tissues with increasing external levels, the proportion retained in the roots, relative to the shoots, was constant (approximately 95%). Two binding characteristics of the Hg within plant tissue were detected. A major proportion of Hg was tightly bound, being unaffected by treatment with ethanol and hydrochloric acid. The remaining Hg in the tissue was removed by either water or hydrochloric acid treatment. Cell fractionation indicated that the major binding component of Hg in plant tissues was the cell wall.