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Fluorimetry Study of N‐(1‐Pyrenyl)iodoacetamide‐Labelled F‐Actin: Local Structural Change of Actin Protomer both on Polymerization and on Binding of Heavy Meromyosin
Open accessAbstract
A fluorescent reagent, N‐(1‐pyrenyl)iodoacetamide, was conjugated to rabbit skeletal muscle actin at the site of the most reactive sulfhydryl group, and fluorescence characteristics (excitation and emission spectra, quantum yields, lifetimes) of the conjugate were investigated. Associated with polymerization of labelled G‐actin, the fluorescence intensity at 407 nm, after excitation at 365 nm, was enhanced by a factor of about 25. It was reduced to about 25% on the binding of heavy meromyosin (or subfragment 1). The results suggest that binding of heavy meromyosin to the protomer of F‐actin alters the local structure of the protomer towards a G‐actin‐like one.
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Cited by
Carlier, M.-F., Laurent, V., Santolini, J., Melki, R., Didry, D., Xia, G.-X., Hong, Y., Chua, N.-H., & Pantaloni, D. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.136.6.1307 · Cited by 1,049
Bubb, M. R., Senderowicz, A. M., Sausville, E. A., Duncan, K. L., & Korn, E. D. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)36545-6 · Cited by 811
Blanchoin, L., Amann, K. J., Higgs, H. N., Marchand, J.-B., Kaiser, D. A., & Pollard, T. D. (2000) · Nature · doi:10.1038/35010008 · Cited by 586
Welch, M. D., Rosenblatt, J., Skoble, J., Portnoy, D. A., & Mitchison, T. J. (1998) · Science · doi:10.1126/science.281.5373.105 · Cited by 508
Microscopic analysis of polymerization dynamics with individual actin filaments
Fujiwara, I., Takahashi, S., Tadakuma, H., Funatsu, T., & Ishiwata, S. (2002) · Nature Cell Biology · doi:10.1038/ncb841 · Cited by 420
Metadata
- Authors
- KOUYAMA, T., & MIHASHI, K.
- Venue
- European Journal of Biochemistry
- Publisher
- Wiley
- Published
- 1981-02
- Volume
- 114
- Issue
- 1
- Pages
- 33-38
- ISSN
- 0014-2956 (ISSN-L)
- 1432-1033
- OpenAlex
- W4297917728
- Cited by
- 714
- References
- 0
- FWCI
- 4.75
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Fluorimetry Study of N‐(1‐Pyrenyl)iodoacetamide‐Labelled F‐Actin: Local Structural Change of Actin Protomer both on Polymerization and on Binding of Heavy Meromyosin. https://app.cochat.ai/papers/10.1111/j.1432-1033.1981.tb06167.x
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- KOUYAMA, T., & MIHASHI, K. (1981). Fluorimetry Study of N‐(1‐Pyrenyl)iodoacetamide‐Labelled F‐Actin: Local Structural Change of Actin Protomer both on Polymerization and on Binding of Heavy Meromyosin. European Journal of Biochemistry. https://doi.org/10.1111/j.1432-1033.1981.tb06167.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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