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Structure and regulation of the sheep metallothionein-Ia gene
Open accessAbstract
Screening of a sheep genomic λ library with a sheep metallothionein‐I cDNA clone resulted in the isolation of a 13,200‐base‐pair fragment containing a metallothionein gene which DNA sequence analysis identified as the gene encoding the cloned cDNA. The two introns occur at identical positions to those in other mammalian metallothioneins but are considerably larger. The first intron contains a DNA element that is present in a related but not identical form in many places in the sheep genome. Comparison of the promoter sequences of this gene (sMT‐Ia) with the promoters of metallothionein genes from other species identified a number of conserved regions which may be important in the regulation of this gene by heavy metals, glucocorticoids and α‐interferon. In sheep fibroblasts, the levels of sMT‐Ia mRNA was found to be maximally elevated (95‐fold) in the presence of zinc or cadmium and elevated 30‐fold in the presence of copper. Dexamethasone had no effect upon mRNA levels. Thus this gene shows a pattern of regulation similar to the human MT‐If gene, but distinct from the other human and mouse metallothionein genes so far reported.
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MT-1 is a zinc, cadmium or copper-inducible sheep metallothionine-Ia promoter of 13,200 bp.
“MT-1 | 13,200 | Zinc, cadmium or copper-inducible sheep metallothionine-Ia promoter | [174]”
From Recombinant Adeno-Associated Viral Vectors (rAAV)-Vector Elements in Ocular Gene Therapy Clinical Trials and Transgene Expression and Bioactivity Assays (2020) · cites this paper as [174]
The Results (p. 2) identify a 13.2-kbp cloned insert containing the sheep MT-Ia gene, not a 13,200-bp promoter. The response section (pp. 5–6) reports increased MT-Ia mRNA in sheep fibroblasts after zinc, cadmium, or copper exposure, but does not establish induction of a promoter of that length. (LLM, conf=0.90)
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Citations
Cites
Detection of specific sequences among DNA fragments separated by gel electrophoresis
Southern, E. M. (1975) · Journal of Molecular Biology · doi:10.1016/s0022-2836(75)80083-0 · Cited by 32,245
Rigby, P. W. J., Dieckmann, M., Rhodes, C., & Berg, P. R. (1977) · Journal of Molecular Biology · doi:10.1016/0022-2836(77)90052-3 · Cited by 11,400
The Metabolic Basis of Inherited Disease.
(1988) · Annals of Internal Medicine · doi:10.7326/0003-4819-108-3-503_7 · Cited by 7,807
[2] New M13 vectors for cloning
Messing, J. W. (1983) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(83)01005-8 · Cited by 5,240
Screening λgt Recombinant Clones by Hybridization to Single Plaques in Situ
Benton, W. D., & Davis, R. W. (1977) · Science · doi:10.1126/science.322279 · Cited by 4,520
Organization and Expression of Eucaryotic Split Genes Coding for Proteins
Breathnach, R., & Chambon, P. (1981) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.50.070181.002025 · Cited by 4,481
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing
Sanger, F., Coulson, A. R., Barrell, B. G., Smith, A. J. H., & Roe, B. A. (1980) · Journal of Molecular Biology · doi:10.1016/0022-2836(80)90196-5 · Cited by 3,407
The Metabolic Basis of Inherited Disease.
(1983) · Annals of Internal Medicine · doi:10.7326/0003-4819-99-3-425_3 · Cited by 2,693
Dynan, W. S., & Tjian, R. (1983) · Cell · doi:10.1016/0092-8674(83)90210-6 · Cited by 1,445
Karin, M., Haslinger, A., Holtgreve, H., Richards, R. I., Krauter, P., Westphal, H. M., & Beato, M. (1984) · Nature · doi:10.1038/308513a0 · Cited by 888
An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences
Staden, R. (1982) · Nucleic Acids Research · doi:10.1093/nar/10.9.2951 · Cited by 799
Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro
Ruskin, B., Krainer, A. R., Maniatis, T., & Green, M. R. (1984) · Cell · doi:10.1016/0092-8674(84)90553-1 · Cited by 740
The making of strand-specific M13 probes
Hu, N.-T., & Messing, J. W. (1982) · Gene · doi:10.1016/0378-1119(82)90143-3 · Cited by 649
Transcriptional regulation of the mouse metallothionein-I gene by heavy metals.
Durnam, D. M., & Palmiter, R. D. (1981) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)69264-1 · Cited by 600
Friedman, R. L., & Stark, G. R. (1985) · Nature · doi:10.1038/314637a0 · Cited by 491
Karin, M., & Richards, R. I. (1982) · Nature · doi:10.1038/299797a0 · Cited by 466
Metallothioneins: Proteins in search of function
Karin, M. (1985) · Cell · doi:10.1016/0092-8674(85)90051-0 · Cited by 442
Nordheim, A., & Rich, A. (1983) · Nature · doi:10.1038/303674a0 · Cited by 435
Stuart, G. W., Searle, P. F., Chen, H. Y., Brinster, R. L., & Palmiter, R. D. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.23.7318 · Cited by 389
Stuart, G. W., Searle, P. F., & Palmiter, R. D. (1985) · Nature · doi:10.1038/317828a0 · Cited by 359
Regulation, Linkage, and Sequence of Mouse Metallothionein I and II Genes
Searle, P. F., Davison, B. L., Stuart, G. W., Wilkie, T. M., Norstedt, G., & Palmiter, R. D. (1984) · Molecular and Cellular Biology · doi:10.1128/mcb.4.7.1221 · Cited by 344
Richards, R. I., Heguy, A., & Karin, M. (1984) · Cell · doi:10.1016/0092-8674(84)90322-2 · Cited by 324
Structure of mouse metallothionein-I gene and its mRNA
Glanville, N., Durnam, D. M., & Palmiter, R. D. (1981) · Nature · doi:10.1038/292267a0 · Cited by 293
Transcriptional regulation of mouse liver metallothionein-I gene by glucocorticoids
Hager, L. J., & Palmiter, R. D. (1981) · Nature · doi:10.1038/291340a0 · Cited by 277
McKnight, S. L., Kingsbury, R. C., Spence, A. M., et al. (1984) · Cell · doi:10.1016/0092-8674(84)90321-0 · Cited by 272
Mayo, K. E., Warren, R., & Palmiter, R. D. (1982) · Cell · doi:10.1016/0092-8674(82)90094-0 · Cited by 247
Cheley, S., & Anderson, R. (1984) · Analytical Biochemistry · doi:10.1016/0003-2697(84)90339-7 · Cited by 243
Underwood, E. J. (1977) · Elsevier eBooks · doi:10.1016/b978-0-12-709065-8.50007-9 · Cited by 210
(1979) · Proceedings of the Fourth International Symposium on Polarization Phenomena in Nuclear Reactions · doi:10.1007/978-3-0348-6493-0 · Cited by 207
Glucocorticoid regulation of metallothionein-I mRNA synthesis in cultured mouse cells.
Mayo, K. E., & Palmiter, R. D. (1981) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)69658-4 · Cited by 200
Ternary Coordination Complex between Human Serum Albumin, Copper (II), and l-Histidine
Lau, S.-J., & Sarkar, B. (1971) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)61817-4 · Cited by 197
Duplicated heavy metal control sequences of the mouse metallothionein-I gene.
Carter, A. D., Felber, B. K., Walling, M. J., et al. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.23.7392 · Cited by 166
Coordinate regulation of mouse metallothionein I and II genes by heavy metals and glucocorticoids.
Yagle, M. K., & Palmiter, R. D. (1985) · Molecular and Cellular Biology · doi:10.1128/mcb.5.2.291 · Cited by 147
Isolation and characterization of six human hepatic isometallothioneins
Hunziker, P., & Kägi, J. H. R. (1985) · Biochemical Journal · doi:10.1042/bj2310375 · Cited by 86
Varshney, U., Jahroudi, N., Foster, R., et al. (1986) · Molecular and Cellular Biology · doi:10.1128/mcb.6.1.26 · Cited by 78
Pearce, P., Khalid, B. A. K., & Funder, J. W. (1981) · Endocrinology · doi:10.1210/endo-109-4-1073 · Cited by 74
cDNa cloning and nucleotide sequence comparison of Chinese hamster metallothionein I and II mRNAs
Griffith, B. B., Walters, R. S., Enger, M. D., Hildebrans, C. E., & Griffith, J. K. (1983) · Nucleic Acids Research · doi:10.1093/nar/11.3.901 · Cited by 69
Initiation and regulation of simian virus 40 early transcription in vitro
Lebowitz, P., & Ghosh, P. K. (1982) · Journal of Virology · doi:10.1128/jvi.41.2.449-461.1982 · Cited by 40
Expression and Hormonal Regulation of the Rat Growth Hormone Gene in Transfected Mouse L Cells
Karin, M., Eberhardt, N. L., Mellon, S. H., et al. (1984) · DNA · doi:10.1089/dna.1984.3.147 · Cited by 36
Whanger, P. D., Oh, S., & Deagen, J. T. (1981) · Journal of Nutrition · doi:10.1093/jn/111.7.1207 · Cited by 23
Cloning and sequencing of a sheep metallothionein cDNA
PETERSON, M. G., Lazdins, I., Danks, D. M., et al. (1984) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1984.tb08399.x · Cited by 21
Self-citationKretschmer, P. J., Kaufman, R. E., Coon, H., et al. (1980) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)85871-4 · Cited by 20
Karin, M., & Holtgreve, H. (1984) · DNA · doi:10.1089/dna.1.1984.3.319 · Cited by 12
Flagged first, then by citations
Research works printed but not linked: 5
- Bremner I., 1980, Ciba Found. Symp., 79, 23
- Maniatis T., 1982, Molecular cloning
- Searle P. F., 1984, Mol. Cell. Biol., 4, 1211
- Fromm M., 1982, J. Mol. Appl. Genet., 1, 457
- Darwish H. M., 1984, Am. J. Physiol., G72
Cited by
Sorted by citations
Buck, T., & Wijnholds, J. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21124197 · Cited by 92
“MT-1 13200 Zinc, cadmium or copper-inducible sheep metallothionine-Ia promoter [174]”
Background
Metadata
- Authors
- PETERSON, M. G., & MERCER, J. F. B.
- Affiliations
- Royal Children's Hospital (AU)
- Corresponding author
- PETERSON, M. Gregory
- Venue
- European Journal of Biochemistry
- Publisher
- Wiley
- Published
- 1986-11
- Volume
- 160
- Issue
- 3
- Pages
- 579-585
- ISSN
- 0014-2956 (ISSN-L)
- 1432-1033
- OpenAlex
- W2070157434
- Cited by
- 37
- References
- 43
- Citations by year
- 2020: 1 · 2012: 1
- FWCI
- 5.83
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- European Journal of Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Figshare (submittedVersion, cc-by)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Keywords
- sheep, gene structure, introns, promoter, heavy metal regulation, zinc, cadmium, copper, glucocorticoids, interferon-alpha, mRNA levels, fibroblasts, gene expression regulation
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA, Genes, Metallothionein, RNA Caps, RNA, Messenger, Sheep, Transcription, Genetic
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