The tricarboxylate carrier from rat liver mitochondria: Purification, reconstitution and kinetic characterization
Abstract
The tricarboxylate carrier from rat liver mitochondria has been purified and reconstituted into phospholipid vesicles. Its activity has been characterized by both a radioactive citrate uptake assay and a coupled enzymatic assay. A K m of 40 1tM and a Vmax of 1.56 p.mol × min−1× mg−1 have been determined for the carrier. Cholesterol levels of between 5–10% of total lipid content are shown to cause a decrease in carrier activity.
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SLC25A1 is sensitive to membrane rigidity, and high levels of cholesterol or acidic phospholipids in the IMM repress mitochondrial citrate export.
“SLC25A1 is sensitive to membrane rigidity, and high levels of cholesterol or acidic phospholipids in the IMM repress mitochondrial citrate export.84”
From The multifaceted contributions of mitochondria to cellular metabolism (2018) · cites this paper as [84]
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The multifaceted contributions of mitochondria to cellular metabolism
Spinelli, J. B., & Haigis, M. C. (2018) · Nature Cell Biology · doi:10.1038/s41556-018-0124-1 · Cited by 1,935 · see citing works
- Authors
- GLERUM, D. M., CLAEYS, D., MERTENS, W., & AZZI, A.
- Affiliations
- University of Bern (CH)
- Corresponding author
- AZZI, Angelo
- Venue
- European Journal of Biochemistry
- Publisher
- Wiley
- Published
- 1990-12
- Volume
- 194
- Issue
- 2
- Pages
- 681-684
- ISSN
- 0014-2956 (ISSN-L)
- 1432-1033
- OpenAlex
- W1577040097
- Cited by
- 23
- References
- 13
- Citations by year
- 2023: 1 · 2022: 1 · 2019: 1 · 2018: 1 · 2017: 1
- FWCI
- 0.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Keywords
- tricarboxylate carrier, liver mitochondria, protein purification, phospholipid vesicles, reconstitution, citrate uptake, kinetic parameters, cholesterol, membrane lipid composition, mitochondrial transport
- MeSH terms
- Animals, Carrier Proteins, Cholesterol, Chromatography, Liquid, Electrophoresis, Polyacrylamide Gel, Kinetics, Mitochondria, Liver, Rats
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