The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Peptide‐N4‐(N‐acetyl‐β‐glucosaminyl)asparagine amidase F cannot release glycans with fucose attached α1 → 3 to the asparagine‐linked N‐acetylglucosamine residue
Open accessAbstract
The ability of peptide‐N4‐(N‐acetyl‐β‐glucosaminyl)asparagine amidase F (PNGase F) from Flavobacterium meningosepticum and PNGase A from sweet almonds to deglycosylate N‐glycopeptides and N‐glycoproteins from plants was compared. Bromelain glycopeptide and horseradish peroxidase‐C glycoprotein, which contain xylose linked β1 → 2 to β‐mannose and fucose linked α1 → 3 to the innermost N‐acetylglucosamine, were used as substrates. In contrast to PNGase A, the enzyme from F. meningosepticum did not act upon these substrates even at concentrations 100‐fold higher than required for complete deglycosylation of commonly used standard substrates. After removal of α1 → 3‐linked fucose from the plant glycopeptide and glycoprotein by mild acid hydrolysis, they were readily degraded by PNGase F at moderate enzyme concentrations. Hence we conclude that α1 → 3 fucosylation of the inner N‐acetylglucosamine impedes the enzymatic action of PNGase F. Knowledge of this limitation of the deglycosylation potential of PNGase F may turn it from a pitfall into a useful experimental tool.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Deglycosylation of asparagine-linked glycans by peptide:N-glycosidase F
Tarentino, A. L., Gomez, C. M., & Plummer, T. H. (1985) · Biochemistry · doi:10.1021/bi00338a028 · Cited by 1,131
Maley, F., Trimble, R. B., Tarentino, A. L., & Plummer, T. H. (1989) · Analytical Biochemistry · doi:10.1016/0003-2697(89)90115-2 · Cited by 797
Plummer, T. H., Elder, J. H., Alexander, S., Phelan, A. W., & Tarentino, A. L. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)90568-5 · Cited by 587
Jones, B. N., & Gilligan, J. P. (1983) · Journal of Chromatography A · doi:10.1016/s0021-9673(01)90918-5 · Cited by 524
WELINDER, K. G. (1979) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1979.tb13061.x · Cited by 417
Demonstration of a new amidase acting on glycopeptides
Takahashi, N. (1977) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(77)90982-2 · Cited by 182
Parente, J. P., Cardon, P., Leroy, Y., Montreuil, J., Fournet, B., & Ricart, G. (1985) · Carbohydrate Research · doi:10.1016/s0008-6215(00)90753-5 · Cited by 179
Ishihara, H., Takahashi, N., Oguri, S., & Tejima, S. (1979) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)86580-8 · Cited by 169
Takahashi, N., Ishii, I., Ishihara, H., Mori, M., Tejima, S., Jefferis, R., Endo, S., & Arata, Y. (1987) · Biochemistry · doi:10.1021/bi00378a023 · Cited by 163
Cited by
Mass spectrometry approaches to glycomic and glycoproteomic analyses
Ruhaak, L. R., Xu, G., Li, Q., Goonatilleke, E., & Lebrilla, C. B. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00732 · Cited by 395
Structural analysis of full-length SARS-CoV-2 spike protein from an advanced vaccine candidate
Bangaru, S., Ozorowski, G., Turner, H. L., Antanasijevic, A., Huang, D., Wang, X., Torres, J. L., Diedrich, J. K., Tian, J.-H., Portnoff, A. D., et al. (2020) · Science · doi:10.1126/science.abe1502 · Cited by 346
High-sensitivity Analytical Approaches for the Structural Characterization of Glycoproteins
Alley, W. R., Mann, B. F., & Novotny, M. V. (2013) · Chemical Reviews · doi:10.1021/cr3003714 · Cited by 310
Tarentino, A. L., & Plummer, T. H. (1994) · Methods in Enzymology · doi:10.1016/0076-6879(94)30006-2 · Cited by 291
Advances in mass spectrometry driven O-glycoproteomics
Levery, S. B., Steentoft, C., Halim, A., Narimatsu, Y., Clausen, H., & Vakhrushev, S. Y. (2015) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2014.09.026 · Cited by 111
Metadata
- Authors
- TRETTER, V., ALTMANN, F., & MÄRZ, L.
- Venue
- European Journal of Biochemistry
- Publisher
- Wiley
- Published
- 1991-08
- Volume
- 199
- Issue
- 3
- Pages
- 647-652
- ISSN
- 0014-2956 (ISSN-L)
- 1432-1033
- OpenAlex
- W2076452068
- Cited by
- 406
- References
- 23
- FWCI
- 2.28
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Peptide‐N4‐(N‐acetyl‐β‐glucosaminyl)asparagine amidase F cannot release glycans with fucose attached α1 → 3 to the asparagine‐linked N‐acetylglucosamine residue. https://app.cochat.ai/papers/10.1111/j.1432-1033.1991.tb16166.x
- The paper
- TRETTER, V., ALTMANN, F., & MÄRZ, L. (1991). Peptide‐N4‐(N‐acetyl‐β‐glucosaminyl)asparagine amidase F cannot release glycans with fucose attached α1 → 3 to the asparagine‐linked N‐acetylglucosamine residue. European Journal of Biochemistry. https://doi.org/10.1111/j.1432-1033.1991.tb16166.x
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
