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Cited by 20Glycosylation and Glycoproteins ResearchAnimal Nutrition and PhysiologyDigestive system and related health

Lectin Histochemical Investigations of the Distal Gut of Chicks with Special Emphasis on the Follicle‐associated Epithelium

Pohlmeyer, I., Jörns, J., Schumacher, U., Van Damme, E. J. M., Peumans, W. J., Pfüller, U., Neumann, U.·2005·Journal of Veterinary Medicine Series A
DOI 10.1111/j.1439-0442.2005.00696.x
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Abstract

Summary: Carbohydrates on epithelial cell surfaces play an important role as attachment sites for different microorganisms like bacteria, viruses and protozoa. To obtain more information about the distribution of carbohydrates on the luminal surface along the intestine, lectin histochemical studies on different gut segments of chicks of different age groups were carried out using a panel of 13 lectins with specificities for Man, Glc, Gal, GalNAc, GlcNAc or GlcNAc oligosaccharides and Sia. Furthermore, we tried to find out whether previously reported specificities of certain lectins for M cells (membranous or multifold cells) in the bursa of Fabricius (BF) can be observed also on M cells of the intestine. As a result we were able to demonstrate binding of all lectins employed in these studies in all investigated gut segments. In some cases, the application of the same lectin led to varying staining intensities of the same histological structures in different age‐groups (e.g. staining of the brush border with WGA, LEA, MAA or Conarva) or different gut segments (e.g. staining of goblet cells with CMA II, LEA and MPA). Hence, terminal carbohydrate residues of glycoconjugates on the intestinal epithelium vary depending on age and organ site. As glycoconjugates can act as attachment sites for microorganisms, these differences in the distribution of sugar residues may be one explanation for the site‐specificity of certain pathogens. Furthermore, the binding of lectins to the follicle‐associated epithelium (FAE) of the BF differs from that to the FAE of the intestine again stressing the site specificity of lectin binding. Thus, up to now no universal M‐cell marker along the chicken intestine exists.

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Crossref · 10.1111/j.1439-0442.2005.00696.x

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  • Lectins selectively bind to carbohydrate residues of epithelial cell glycocalyx and can be used on particle surfaces to target M cells.

    “Lectins are proteins that selectively bind to carbohydrate residues of epithelial cells’ glycocalyx and may serve as molecules on particle surface for specifically targeting M cells [103,104].”

    From Use of lectin-functionalized particles for oral immunotherapy (2012) · cites this paper as [103]

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Authors
Pohlmeyer, I., Jörns, J., Schumacher, U., Van Damme, E. J. M., Peumans, W. J., Pfüller, U., & Neumann, U.
Affiliations
  • University of Veterinary Medicine Hannover, Foundation (DE)
  • University Medical Center Hamburg-Eppendorf (DE)
  • Ghent University (BE)
  • Witten/Herdecke University (DE)
Corresponding author
Pohlmeyer, I.
ORCID
Schumacher, U.
Venue
Journal of Veterinary Medicine Series A
Publisher
Wiley
Published
2005-04
Volume
52
Issue
3
Pages
138-146
ISSN
  • 0177-0543 (ISSN-L)
  • 0931-184X
DOI
10.1111/j.1439-0442.2005.00696.x
OpenAlex
W2047419150
Cited by
20
References
43
Citations by year
2025: 1 · 2024: 1 · 2022: 1 · 2019: 2 · 2016: 1 · 2015: 2 · 2013: 1 · 2012: 1
FWCI
0.63
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 17 d)
Locations
  • Zentralblatt für Veterinärmedizin Reihe A (publishedVersion)
  • PubMed (publishedVersion)
  • Ghent University Academic Bibliography (Ghent University) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Digestive system and related health
Keywords
lectin histochemistry, chick intestine, follicle-associated epithelium, bursa of Fabricius, M cells, glycoconjugates, brush border, goblet cells, lectin specificity, age-related changes
MeSH terms
Animals, Bursa of Fabricius, Carbohydrates, Cecum, Chickens, Epithelium, Glycoconjugates, Histocytochemistry, Ileum, Intestinal Mucosa, Lymphocytes, Specific Pathogen-Free Organisms, Lectins, Carbohydrate Metabolism

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