CoChat
Sign Up
Cited by 253Cellular transport and secretionDrug Transport and Resistance MechanismsImmunotherapy and Immune Responses

Transport of membrane-bound macromolecules by M cells in follicle-associated epithelium of rabbit Peyer's patch

Neutra, MarianR., Phillips, TeresaL., Mayer, EllenL., Fishkind, DouglasJ.·1987·Cell and Tissue Research
DOI 10.1007/bf00215747
Open this paper in CoChat — free account

Abstract

M cells in Peyer's patch epithelium conduct transepithelial transport of luminal antigens to cells of the mucosal immune system. To determine the distribution of specific lectin-binding sites on luminal membranes of living M cells and to follow the transport route of membrane-bound molecules, lectin-ferritin conjugates and cationized ferritin were applied to rabbit Peyer's patch mucosa in vivo and in vitro. The degree to which binding enhances transport was estimated by comparing quantitatively the transport of an adherent probe, wheat germ agglutinin-ferritin, to that of a nonadherent BSA-colloidal gold probe. When applied to fixed tissue, the lectins tested bound equally well to M cells and columnar absorptive cells. On living mucosa, however, ferritin conjugates of wheat germ agglutinin and Ricinus communis agglutinins I and II bound more avidly to M cells. Absorptive cells conducted little uptake and no detectable transepithelial transport. Lectins on M cell membranes were endocytosed from coated pits, rapidly transported in a complex system of tubulocisternae and vesicles, and remained adherent to M cell basolateral membranes. Cationized ferritin adhered to anionic sites and was similarly transported, but was released as free clusters at M cell basolateral surfaces. When applied simultaneously to Peyer's patch mucosa, wheat germ agglutinin-ferritin was transported about 50 times more efficiently than was bovine serum albumin-colloidal gold.

Integrity signals

Registry

Crossref · 10.1007/bf00215747

Cites

40 of 40 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • After binding to the apical side of M cells, macromolecules are endocytosed via clathrin-coated vesicles, reach the lysosomal compartment, and are exocytosed basolaterally to be taken up by APCs.

    “Once bound to the apical side of M cells, macromolecules are endocytosed via clathrin-coated vesicles, thereby reaching the lysosomal compartment, from which they are exocytosed to the basolateral site to be taken up by antigen presenting cells (APCs) [34,35].”

    From Use of lectin-functionalized particles for oral immunotherapy (2012) · cites this paper as [34]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Neutra, M., Phillips, T., Mayer, E., & Fishkind, D.
Affiliations
Harvard University (US)
Venue
Cell and Tissue Research
Publisher
Springer Science and Business Media LLC
Published
1987-03
Volume
247
Issue
3
Pages
537-546
ISSN
  • 0302-766X (ISSN-L)
  • 1432-0878
DOI
10.1007/bf00215747
OpenAlex
W1963689848
Cited by
253
References
40
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 1 · 2020: 6 · 2019: 4 · 2018: 4 · 2017: 3 · 2016: 4 · 2015: 7 · 2014: 8 · 2013: 3 · 2012: 4
FWCI
3.16
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Cell and Tissue Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
Keywords
M cells, Peyer's patches, follicle-associated epithelium, transepithelial transport, lectin binding sites, wheat germ agglutinin, Ricinus communis agglutinin, cationized ferritin, basolateral membrane, endocytosis, coated pits, absorptive cells, antigen transport, mucosal immunity
MeSH terms
Animals, Biological Transport, Epithelial Cells, Epithelium, Ferritins, Ileum, Microscopy, Electron, Peyer's Patches, Rabbits, Ricin, Wheat Germ Agglutinins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review