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Cited by 19Ion channel regulation and functionCalcium signaling and nucleotide metabolismMitochondrial Function and Pathology

Depolarization evokes different patterns of calcium signals and exocytosis in bovine and mouse chromaffin cells: The role of mitochondria

Alés, E., Fuentealba, J., García, A. G., López, M. G.·2005·European Journal of Neuroscience
DOI 10.1111/j.1460-9568.2004.03861.x
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Abstract

This study was planned on the assumptions that different high-voltage activated calcium channels and/or the ability of mitochondria to take up Ca(2+) could be responsible for different cytosolic Ca(2+) concentrations ([Ca(2+)](c)) and catecholamine release responses in adrenal chromaffin cells of bovine and mouse species. Short K(+) pulses (2-5 s, 70 mM K(+)) increased [Ca(2+)](c) to a peak of about 1 microM; however, in bovine cells the decline was slower than in mouse cells. Secretory responses were faster in mouse but were otherwise quantitatively similar. Upon longer K(+) applications (1 min), elevations of [Ca(2+)](c) and secretion were prolonged in bovine cells; in contrast [Ca(2+)](c) in mouse cells declined three-fold faster and failed to sustain a continued secretion. Confocal [Ca(2+)](c) imaging following a 50-ms depolarizing pulse showed a similar Ca(2+) entry, but a rate of [Ca(2+)](c) increase and a maximum peak significantly higher in bovine cells; the rate of dissipation of the Ca(2+) wave was faster in the mouse. The mitochondrial protonophore CCCP (2 microm) halved the K(+)-evoked [Ca(2+)](c) and secretory signals in mouse cells, but had little affect on bovine responses. We conclude that the relative densities of L (15% in bovine and 50% in mouse) and P/Q Ca(2+) channels (50% in bovine and 15% in mouse) do not contribute to the observed differences; rather, the different intracellular distribution of Ca(2+), which is strongly influenced by mitochondria, is responsible for a more sustained secretory response in bovine, and for a faster and more transient secretory response in mouse chromaffin cells. It seems that mitochondria near the plasmalemma sequester Ca(2+) more rapidly and efficiently in the mouse than in the bovine chromaffin cell.

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Crossref · 10.1111/j.1460-9568.2004.03861.x

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  • Use of CCCP or FCCP decreases the Ca2+ buffering capacity of mitochondria and increases global exocytosis in bovine and rat chromaffin cells but not in the same cell type in mice.

    “In accordance with this theory, use of CCCP or FCCP decreases the Ca2+ buffering capacity of mitochondria and increases global exocytosis in bovine [164,165] and rat chromaffin cells [166] but not in the same cell type in mice [167], suggesting species differences.”

    From Mitochondrial Uncoupling: A Key Controller of Biological Processes in Physiology and Diseases (2019) · cites this paper as [167]

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Authors
Alés, E., Fuentealba, J., García, A. G., & López, M. G.
Affiliations
  • Universidad Autónoma de Madrid (ES)
  • Hospital Universitario de La Princesa (ES)
Corresponding author
López, M. G.
ORCID
Venue
European Journal of Neuroscience
Publisher
Wiley
Published
2005-01
Volume
21
Issue
1
Pages
142-150
ISSN
  • 0953-816X (ISSN-L)
  • 1460-9568
DOI
10.1111/j.1460-9568.2004.03861.x
OpenAlex
W2004642771
Cited by
19
References
32
Citations by year
2023: 1 · 2022: 1 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 1 · 2013: 1
FWCI
1.00
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 9 d)
Locations
  • European Journal of Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
Keywords
chromaffin cells, catecholamine release, calcium signaling, mitochondrial calcium uptake, depolarization, L-type calcium channels, P/Q-type calcium channels, high-voltage-activated calcium channels, intracellular calcium, species differences, cattle, mice, adrenal medulla
MeSH terms
Aniline Compounds, Animals, Calcium, Carbonyl Cyanide m-Chlorophenyl Hydrazone, Catecholamines, Cattle, Drug Interactions, Electrochemistry, Exocytosis, Ionophores, Membrane Potentials, Mitochondria, Potassium, Xanthenes, Patch-Clamp Techniques, Microscopy, Confocal, Chromaffin Cells, Calcium Signaling, Mice

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