Adenosine A1receptor‐mediated inhibition of dopamine release from rat striatal slices is modulated by D1dopamine receptors
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Dopamine release is regulated by presynaptic dopamine receptors and interactions between adenosine and dopamine receptors have been well documented. In the present study, dopamine release from isolated striatal slices from Wistar rats was measured using fast cyclic voltammetry. Single‐pulse stimulation (0.1 ms, 10 V) was applied every 5 min over a 2‐h period. Superfusion with the adenosine (A)1 receptor agonist N 6‐cyclopentyladenosine (CPA), but not the A 2 receptor agonist 3‐[4‐[2‐[[6‐amino‐9‐[(2R,3R,4S,5S)‐5‐(ethylcarbamoyl)‐3,4‐dihydroxy‐oxolan‐2‐yl]purin‐2‐yl]amino]ethyl] phenyl]propanoic acid (CGS 21680), inhibited dopamine release in a concentration‐dependent manner (IC 50 3.80 × 10−7 m;n = 10). The dose–response curve to CPA was shifted to the right (IC 50 6.57 × 10−6 m;n = 6,P < 0.05 vs. control) by the A 1 receptor antagonist 8‐cyclopentyl‐1,3‐dipropylxanthine (DPCPX). Neither the D 1 agonist 6‐chloro‐APB nor the D 1 antagonist R‐(+)‐8‐chloro‐2,3,4,5‐tetrahydro‐3‐methyl‐5‐phenyl‐1H‐3‐ benzazepine‐7‐ol (SCH 23390) altered dopamine release on their own. However, SCH 23390 (3 µ m) significantly attenuated the response to CPA (IC 50 1.44 × 10−5 m;n = 6,P < 0.01 vs. control). Furthermore, the inhibitory effect of CPA was significantly increased in the presence of 6‐chloro‐APB (1 µ m). In radioligand binding experiments, CPA interacted with high‐ and low‐affinity states of [3 H]DPCPX‐lableled A 1 receptors. The high‐affinity agonist binding to A 1 receptors was inhibited by the stable guanosine triphosphate analogue Gpp(NH)p. In contrast, neither the proportion nor the affinity of high‐affinity A 1 receptors was altered by dopamine or SCH 23390. These results provide evidence that the inihibition of dopamine release by adenosine A 1 receptors is dependent, at least in part, on the simultaneous activation of D 1 dopamine receptors. While the mechanism underlying this interaction remains to be determined, it does not appear to involve an intramembrane interaction between A 1 and D 1 receptors.
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Adenosine, particularly through A1 receptor activation, inhibits release of neurotransmitters involved in central excitability and neural network regulation, including norepinephrine, acetylcholine, dopamine, and glutamate.
“Adenosine, particularly through A1 receptor activation, inhibits the release of neurotransmitters involved in central excitability and neural network regulation, including norepinephrine [32], acetylcholine [33,34], dopamine [35], and glutamate [36].”
From Pre-Exercise Caffeine as a Modulator of Exercise-Induced Acute Cardiometabolic Responses: A Narrative Review (2026) · cites this paper as [35]
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Cites
Dopamine receptors: from structure to function
MISSALE, C., NASH, S. R., ROBINSON, S. W., JABER, M., & CARON, M. G. (1998) · Physiological Reviews · doi:10.1152/physrev.1998.78.1.189 · Cited by 3,633 · see citing works
Samama, P., Cotecchia, S., Costa, T., & Lefkowitz, R. J. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)53442-6 · Cited by 1,256 · see citing works
Adenosine–dopamine receptor–receptor interactions as an integrative mechanism in the basal ganglia
Ferré, S., Fuxé, K., Fredholm, B. B., Morelli, M., & Popoli, P. (1997) · Trends in Neurosciences · doi:10.1016/s0166-2236(97)01096-5 · Cited by 825 · see citing works
Burst firing in midbrain dopaminergic neurons
Overton, P. G., & Clark, D. (1997) · Brain Research Reviews · doi:10.1016/s0165-0173(97)00039-8 · Cited by 466 · see citing works
Armstrong‐James, M., & Millar, J. (1979) · Journal of Neuroscience Methods · doi:10.1016/0165-0270(79)90039-6 · Cited by 382 · see citing works
Venton, B. J., Zhang, H., Garris, P. A., Phillips, P. E. M., Sulzer, D., & Wightman, R. M. (2003) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2003.02109.x · Cited by 281 · see citing works
Fuxé, K., Ferré, S., Zoli, M., & Agnati, L. F. (1998) · Brain Research Reviews · doi:10.1016/s0165-0173(97)00049-0 · Cited by 277 · see citing works
Link between D1 and D2 dopamine receptors is reduced in schizophrenia and Huntington diseased brain.
Seeman, P. M., Niznik, H. B., Guan, H. C., Booth, G. L., & Ulpian, C. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.24.10156 · Cited by 190 · see citing works
Purinoceptors in the Nervous System*
Fredholm, B. B. (1995) · Pharmacology & Toxicology · doi:10.1111/j.1600-0773.1995.tb00135.x · Cited by 173 · see citing works
Evidence for Adenosine/Dopamine Receptor Interactions Indications for Heteromerization
Franco, R. (2000) · Neuropsychopharmacology · doi:10.1016/s0893-133x(00)00144-5 · Cited by 172 · see citing works
Rao, P. A., Molinoff, P. B., & Joyce, J. N. (1991) · Developmental Brain Research · doi:10.1016/0165-3806(91)90045-k · Cited by 165 · see citing works
Pombal, M. A., & Puelles, L. (1999) · The Journal of Comparative Neurology · doi:10.1002/(sici)1096-9861(19991122)414:3 · Cited by 160 · see citing works
Adenosine A1 and A2 receptors modulate extracellular dopamine levels in rat striatum
Okada, M., Mizuno, K., & Kaneko, S. (1996) · Neuroscience Letters · doi:10.1016/0304-3940(96)12780-4 · Cited by 158 · see citing works
Stimulated D1 dopamine receptors couple to multiple Gα proteins in different brain regions
Jin, L., Wang, H., & Friedman, E. (2001) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2001.00470.x · Cited by 142 · see citing works
David, H., Ansseau, M., & Abraini, J. H. (2005) · Brain Research Reviews · doi:10.1016/j.brainresrev.2005.09.002 · Cited by 139 · see citing works
Postsynaptic antagonistic interaction between adenosine A1, and dopamine D1 receptors
Ferré, S., Popoli, P., Giménez‐Llort, L., Finnman, U.-B., Martı́nez, E., de Carolis, A. S., & Fuxé, K. (1994) · Neuroreport · doi:10.1097/00001756-199412300-00020 · Cited by 138 · see citing works
Current Evidence for Neuroprotective Effects of Nicotine and Caffeine Against Parkinson??s Disease
Ross, G. W., & Petrovitch, H. (2001) · Drugs & Aging · doi:10.2165/00002512-200118110-00001 · Cited by 137 · see citing works
Okada, M., Kiryu, K., 河田, 祐., Mizuno, K., Wada, K., Tasaki, H., & Kaneko, S. (1997) · European Journal of Pharmacology · doi:10.1016/s0014-2999(96)00938-7 · Cited by 130 · see citing works
Receptor-receptor interactions as an integrative mechanism in nerve cells
Zoli, M., Agnati, L. F., Hedlund, P. B., Li, X., Ferré, S., & Fuxé, K. (1993) · Molecular Neurobiology · doi:10.1007/bf02769180 · Cited by 129 · see citing works
Quarta, D., Borycz, J., Solinas, M., Patkar, K. A., Hockemeyer, J., Ciruela, F., Lluı́s, C., Franco, R., Woods, A. S., Goldberg, S. R., et al. (2004) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2004.02761.x · Cited by 120 · see citing works
Intramembrane receptor–receptor interactions: a novel principle in molecular medicine
Fuxé, K., Canals, M., Torvinen, M., Marcellino, D. J., Terasmaa, A., Genedani, S., Leo, G., Guidolin, D., Dı́az-Cabiale, Z., Rivera, A., et al. (2006) · Journal of Neural Transmission · doi:10.1007/s00702-006-0589-0 · Cited by 118 · see citing works
Jin, S., Johansson, B. A., & Fredholm, B. B. (1993) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)39384-5 · Cited by 115 · see citing works
Palij, P., Bull, D. R., Sheehan, M. J., et al. (1990) · Brain Research · doi:10.1016/0006-8993(90)90329-a · Cited by 107 · see citing works
Caffeine reverses the memory disruption induced by intra-nigral MPTP-injection in rats
Gevaerd, M. S., Takahashi, R. N., Silveira, R., & Cunha, C. D. (2001) · Brain Research Bulletin · doi:10.1016/s0361-9230(01)00501-9 · Cited by 104 · see citing works
Johansson, B. A., Georgiev, V., & Fredholm, B. B. (1997) · Neuroscience · doi:10.1016/s0306-4522(97)00143-7 · Cited by 97 · see citing works
Firing patterns of midbrain dopamine neurons: differences between A9 and A10 cells
Grenhoff, J., Ugedo, L., & Svensson, T. H. (1988) · Acta Physiologica Scandinavica · doi:10.1111/j.1748-1716.1988.tb08468.x · Cited by 83 · see citing works
Franco, R., Lluı́s, C., Canela, E. I., Mallol, J., Agnati, L. F., Casadó, V., Ciruela, F., Ferré, S., & Fuxé, K. (2006) · Journal of Neural Transmission · doi:10.1007/s00702-006-0566-7 · Cited by 78 · see citing works
Limberger, N., Trout, S. J., Kruk, Z. L., et al. (1991) · Naunyn-Schmiedeberg s Archives of Pharmacology · doi:10.1007/bf00174745 · Cited by 75 · see citing works
Fast cyclic voltammetry: Measuring transmitter release in ‘real time’
Stamford, J. A. (1990) · Journal of Neuroscience Methods · doi:10.1016/0165-0270(90)90043-f · Cited by 74 · see citing works
Phillips, P. E. M., & Stamford, J. A. (2000) · Brain Research · doi:10.1016/s0006-8993(00)02958-9 · Cited by 66 · see citing works
Wood, P. L. (1989) · Neuropharmacology · doi:10.1016/0028-3908(89)90062-2 · Cited by 60 · see citing works
Stamford, J. A., Muscat, R., O'Connor, J. J., Patel, J. C., Trout, S. J., Wieczorek, W. J., Kruk, Z. L., & Willner, P. (1991) · Psychopharmacology · doi:10.1007/bf02244322 · Cited by 58 · see citing works
Self-citationMaemoto, T., Finlayson, K., Olverman, H. J., Akahane, A., Horton, R. W., & Butcher, S. P. (1997) · British Journal of Pharmacology · doi:10.1038/sj.bjp.0701465 · Cited by 57 · see citing works
Torvinen, M., Ginés, S., Hillion, J., Latini, S., Canals, M., Ciruela, F., Bordoni, F., Staines, W. A., Pedata, F., Agnati, L. F., et al. (2002) · Neuroscience · doi:10.1016/s0306-4522(02)00058-1 · Cited by 57 · see citing works
Yabuuchi, K., Kuroiwa, M., Shuto, T., et al. (2006) · Neuroscience · doi:10.1016/j.neuroscience.2006.04.047 · Cited by 55 · see citing works
Gołembiowska, K., & Żylewska, A. (1998) · Brain Research · doi:10.1016/s0006-8993(98)00743-4 · Cited by 51 · see citing works
Ballarín, M., Reiriz, J., Ambrosio, S., & Mahy, N. (1995) · Neuroscience Letters · doi:10.1016/0304-3940(94)11217-7 · Cited by 49 · see citing works
The role of dopamine in the locomotor stimulant effects and tolerance to these effects of caffeine
Powell, K. R., Iuvone, P. M., & Holtzman, S. G. (2001) · Pharmacology Biochemistry and Behavior · doi:10.1016/s0091-3057(01)00497-x · Cited by 39 · see citing works
Whitehead, K. J., Rose, S., & Jenner, P. G. (2001) · European Journal of Neuroscience · doi:10.1046/j.0953-816x.2001.01702.x · Cited by 29 · see citing works
Abbracchio, M. P., Colombo, F., Luca, M. D., et al. (1987) · Pharmacological Research Communications · doi:10.1016/0031-6989(87)90085-3 · Cited by 29 · see citing works
Bull, D. R., Bakhtiar, R., & Sheehan, M. J. (1991) · Neuroscience Letters · doi:10.1016/0304-3940(91)90504-m · Cited by 27 · see citing works
Ambrósio, A. F., Malva, J. O., Carvalho, A. P., & Carvalho, C. M. (1996) · Neuroscience Letters · doi:10.1016/s0304-3940(96)13252-3 · Cited by 26 · see citing works
Acquas, E., & Chiara, G. D. (2001) · Neurological Sciences · doi:10.1007/s100720170037 · Cited by 16 · see citing works
Fast electrochemical studies in isolated tissues
Kruk, Z. L., & O'Connor, J. J. (1995) · Trends in Pharmacological Sciences · doi:10.1016/s0165-6147(00)89005-7 · Cited by 14 · see citing works
Self-citationKombian, S. B., Ananthalakshmi, K. V. V., & Edafiogho, I. O. (2006) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.2006.05152.x · Cited by 13 · see citing works
Farrell, C. B., Lawlor, M., Dunne, A., et al. (1995) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1995.65031124.x · Cited by 4 · see citing works
Iyengar, S. (1989) · Neuropharmacology · doi:10.1016/0028-3908(89)90063-4 · Cited by 2 · see citing works
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- Authors
- O'Neill, C., Nolan, B. J., Macari, A., O'Boyle, K. M., & O'Connor, J. J.
- Affiliations
- University College Dublin (IE)
- Corresponding author
- O'Connor, J. J.
- Venue
- European Journal of Neuroscience
- Publisher
- Wiley
- Published
- 2007-12
- Volume
- 26
- Issue
- 12
- Pages
- 3421-3428
- ISSN
- 0953-816X (ISSN-L)
- 1460-9568
- OpenAlex
- W2111835847
- Cited by
- 37
- References
- 47
- Citations by year
- 2026: 1 · 2024: 1 · 2022: 1 · 2021: 2 · 2020: 2 · 2017: 2 · 2016: 5 · 2015: 4 · 2014: 3 · 2013: 3 · 2012: 1
- FWCI
- 1.86
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- European Journal of Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Adenosine and Purinergic Signaling
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- adenosine A1 receptor, dopamine release, dopamine D1 receptor, striatum, presynaptic dopamine receptors, N6-cyclopentyladenosine, DPCPX, SCH 23390
- MeSH terms
- Adenosine, Animals, Corpus Striatum, Dopamine, Electric Stimulation, Ligands, Male, Rats, Wistar, Receptors, Dopamine D1, Dopamine Agonists, Dopamine Antagonists, Receptor, Adenosine A1, Rats, Adenosine A1 Receptor Agonists, Adenosine A1 Receptor Antagonists, In Vitro Techniques
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