The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Signalization and cytoskeleton activity through myosin IB during the early steps of phagocytosis in Entamoeba histolytica: a proteomic approach
Open accessAbstract
Phagocytosis of human cells is a crucial activity for the virulence of the human parasite Entamoeba histolytica. This protozoan invades and destroys the intestine by killing and phagocytosing epithelial cells, erythrocytes and cells from the immune system. In this study, we used magnetic beads covered with proteins from human serum as a model system to study the early events involved in phagocytosis by E. histolytica. We validated the system showing that the beads uptake triggered the activation of the actin-myosin cytoskeleton and involved a PI3-kinase as previously described for erythrophagocytosis. We purified early phagosomes from wild-type (WT) amoeba and from parasites that overproduced myosin IB (MyoIB+), the unique unconventional myosin of E. histolytica. The MyoIB+ cells exhibit a slower and more synchronized uptake process than the WT strain. Proteomic analysis by liquid chromatography and tandem mass spectroscopy (LC-MS/MS) of the WT and MyoIB+ phagosomes allowed us to identify, for the first time, molecular actors involved in the early step of the uptake process. These include proteins involved in cytoskeleton activity, signalling, endocytosis, lytic activity and cell surface proteins. Interestingly, the proteins that we found specifically recruited on the phagosomes from the MyoIB+ strain were previously described in other eukarytotic cells, as involved in the regulation of cortical F-actin dynamics, such as alpha-actinin and formins. This proteomics approach allows a step further towards the understanding of the molecular mechanisms involved in phagocytosis in E. histolytica that revealed some interesting differences compared with phagocytosis in macrophages or Dictyostelium discoideum, and allowed to identify putative candidates for proteins linked to myosin IB activity during the phagocytic process.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Morrissey, J. H. (1981) · Analytical Biochemistry · doi:10.1016/0003-2697(81)90783-1 · Cited by 4,061
A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba
Diamond, L. S., Harlow, D. R., & Cunnick, C. C. (1978) · Transactions of the Royal Society of Tropical Medicine and Hygiene · doi:10.1016/0035-9203(78)90144-x · Cited by 1,878
Proteins of the ADF/Cofilin Family: Essential Regulators of Actin Dynamics
Bamburg, J. R. (1999) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.15.1.185 · Cited by 1,015
Reconstitution of actin-based motility of Listeria and Shigella using pure proteins
Loisel, T. P., Boujemaa‐Paterski, R., Pantaloni, D., & Carlier, M. (1999) · Nature · doi:10.1038/44183 · Cited by 970
The genome of the protist parasite Entamoeba histolytica
Loftus, B., Anderson, I., Davies, R., Alsmark, U. C. M., Samuelson, J. C., Amedeo, P., Roncaglia, P., Berriman, M., Hirt, R. P., Mann, B. J., et al. (2005) · Nature · doi:10.1038/nature03291 · Cited by 824
Self-citationVieira, O. V., Botelho, R. J., Rameh, L. E., Brachmann, S. M., Matsuo, T., Davidson, H. W., Schreiber, A. D., Backer, J., Cantley, L. C., & Grinstein, S. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.200107069 · Cited by 530
Phosphoinositide 3‐kinase‐dependent activation of Rac
Welch, H. C. E., Coadwell, W. J., Stephens, L., & Hawkins, P. T. (2003) · FEBS Letters · doi:10.1016/s0014-5793(03)00454-x · Cited by 320
Involvement of the Arp2/3 complex in phagocytosis mediated by FcγR or CR3
May, R., Caron, E., Hall, A., & Machesky, L. (2000) · Nature Cell Biology · doi:10.1038/35008673 · Cited by 313
Actin Polymerization-Driven Molecular Movement of mDia1 in Living Cells
Higashida, C., Miyoshi, T., Fujita, A., Oceguera-Yañez, F., Monypenny, J., Andou, Y., Narumiya, S., & Watanabe, N. (2004) · Science · doi:10.1126/science.1093923 · Cited by 313
Rabinowitz, S., Horstmann, H., Gordon, S., & Griffiths, G. (1992) · The Journal of Cell Biology · doi:10.1083/jcb.116.1.95 · Cited by 271
Cited by
Sorted by citations
The origins of phagocytosis and eukaryogenesis
Yutin, N., Wolf, M. Y., Wolf, Y. I., & Koonin, E. V. (2009) · Biology Direct · doi:10.1186/1745-6150-4-9 · Cited by 247
“To delineate the complement of the evolutionarily conserved protein components of the phagosome, we compared the sets of proteins identified in proteomic studies of phagosomes isolated from mouse macrophages [53,54], Drosophila S2 cells [55], Dictyostelium discoideum [56], Entamoeba histolytica [57,58], and Tetrahymen…”
BackgroundResultStructure and Content of the Entamoeba histolytica Genome
Clark, C. G., Alsmark, U. C. M., Tazreiter, M. H., Saito‐Nakano, Y., Ali, V., Marion, S., Weber, C., Mukherjee, C., Bruchhaus, I., Tannich, E., et al. (2007) · Advances in Parasitology/Advances in parasitology · doi:10.1016/s0065-308x(07)65002-7 · Cited by 188
Self-citationThe dynamic phagosomal proteome and the contribution of the endoplasmic reticulum
Rogers, L. D., & Foster, L. J. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0705801104 · Cited by 125
Membrane proteomics of phagosomes suggests a connection to autophagy
Shui, W., Sheu, L., Liu, J., Smart, B., Petzold, C. J., Hsieh, T.-Y., Pitcher, A., Keasling, J. D., & Bertozzi, C. R. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0809218105 · Cited by 114
Boettner, D. R., Huston, C. D., Linford, A. S., Buss, S. N., Houpt, E. R., Sherman, N. E., & Petri, W. A. (2008) · PLoS Pathogens · doi:10.1371/journal.ppat.0040008 · Cited by 101
Metadata
- Authors
- Marion, S., Laurent, C., & Guillén, N.
- Affiliations
- Inserm (FR)
- Corresponding author
- Guillén, Nancy
- Venue
- Cellular Microbiology
- Publisher
- Wiley
- Published
- 2005-07-20
- Volume
- 7
- Issue
- 10
- Pages
- 1504-1518
- ISSN
- 1462-5814 (ISSN-L)
- OpenAlex
- W1590737147
- Cited by
- 125
- References
- 50
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 3 · 2023: 1 · 2022: 4 · 2021: 2 · 2020: 6 · 2019: 8 · 2018: 5 · 2017: 3 · 2016: 7 · 2015: 9 · 2014: 9 · 2013: 4 · 2012: 8
- FWCI
- 6.18
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cellular Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- European Commission
- Fondation pour la Recherche Médicale
- Topics
- Health Sciences › Medicine › Infectious Diseases › Amoebic Infections and Treatments
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Protist diversity and phylogeny
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- Entamoeba histolytica, phagocytosis, unconventional myosins, actin-myosin cytoskeleton, PI3-kinase, erythrophagocytosis, alpha-actinin, formins, signal transduction
- MeSH terms
- Actins, Animals, Chromatography, Liquid, Cytoskeleton, Dictyostelium, Entamoeba histolytica, Humans, Microscopy, Fluorescence, Phagocytosis, Phagosomes, Mass Spectrometry, Virulence, Signal Transduction, Genes, Protozoan, Proteome, Myosin Type I
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
