Cited by 125Amoebic Infections and TreatmentsProtist diversity and phylogenyCellular transport and secretion

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Signalization and cytoskeleton activity through myosin IB during the early steps of phagocytosis in Entamoeba histolytica: a proteomic approach

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Marion, Sabrina, Laurent, Christine, Guillén, Nancy·2005·Cellular Microbiology

DOI 10.1111/j.1462-5822.2005.00573.x

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Abstract

Phagocytosis of human cells is a crucial activity for the virulence of the human parasite Entamoeba histolytica. This protozoan invades and destroys the intestine by killing and phagocytosing epithelial cells, erythrocytes and cells from the immune system. In this study, we used magnetic beads covered with proteins from human serum as a model system to study the early events involved in phagocytosis by E. histolytica. We validated the system showing that the beads uptake triggered the activation of the actin-myosin cytoskeleton and involved a PI3-kinase as previously described for erythrophagocytosis. We purified early phagosomes from wild-type (WT) amoeba and from parasites that overproduced myosin IB (MyoIB+), the unique unconventional myosin of E. histolytica. The MyoIB+ cells exhibit a slower and more synchronized uptake process than the WT strain. Proteomic analysis by liquid chromatography and tandem mass spectroscopy (LC-MS/MS) of the WT and MyoIB+ phagosomes allowed us to identify, for the first time, molecular actors involved in the early step of the uptake process. These include proteins involved in cytoskeleton activity, signalling, endocytosis, lytic activity and cell surface proteins. Interestingly, the proteins that we found specifically recruited on the phagosomes from the MyoIB+ strain were previously described in other eukarytotic cells, as involved in the regulation of cortical F-actin dynamics, such as alpha-actinin and formins. This proteomics approach allows a step further towards the understanding of the molecular mechanisms involved in phagocytosis in E. histolytica that revealed some interesting differences compared with phagocytosis in macrophages or Dictyostelium discoideum, and allowed to identify putative candidates for proteins linked to myosin IB activity during the phagocytic process.

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Authors: Marion, S., Laurent, C., & Guillén, N.
Affiliations: Inserm (FR)
Corresponding author: Guillén, Nancy
ORCID: Marion, Sabrina
Guillén, Nancy
Venue: Cellular Microbiology
Publisher: Wiley
Published: 2005-07-20
Volume: 7
Issue: 10
Pages: 1504-1518
ISSN: 1462-5814 (ISSN-L)
DOI: 10.1111/j.1462-5822.2005.00573.x
OpenAlex: W1590737147
Cited by: 125
References: 50
Citations by year: 2026: 1 · 2025: 3 · 2024: 3 · 2023: 1 · 2022: 4 · 2021: 2 · 2020: 6 · 2019: 8 · 2018: 5 · 2017: 3 · 2016: 7 · 2015: 9 · 2014: 9 · 2013: 4 · 2012: 8
FWCI: 6.18
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: doi.wiley.com/10.1002/tdm_license_1.1 (tdm, 3695 d)
Locations: Cellular Microbiology (publishedVersion)
PubMed (publishedVersion)
Funders: European Commission
Fondation pour la Recherche Médicale
Topics: Health Sciences › Medicine › Infectious Diseases › Amoebic Infections and Treatments
Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Protist diversity and phylogeny
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Keywords: Entamoeba histolytica, phagocytosis, unconventional myosins, actin-myosin cytoskeleton, PI3-kinase, erythrophagocytosis, alpha-actinin, formins, signal transduction
MeSH terms: Actins, Animals, Chromatography, Liquid, Cytoskeleton, Dictyostelium, Entamoeba histolytica, Humans, Microscopy, Fluorescence, Phagocytosis, Phagosomes, Mass Spectrometry, Virulence, Signal Transduction, Genes, Protozoan, Proteome, Myosin Type I

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Signalization and cytoskeleton activity through myosin IB during the early steps of phagocytosis in Entamoeba histolytica: a proteomic approach

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