Extracellular protease from Mucor pusillus: purification and characterization
Abstract
Extracellular protease from Mucor pusillus was purified 18‐fold with 7.56% recovery by ion‐exchange chromatography and gel filtration. The enzyme was found to be monomeric in nature, having a molecular mass of 49 kDa. The enzyme acted optimally at 50°C and was stable in the temperature range 30–50°C. It was completely inactivated by heating for 30 min at 65°C. The optimum of activity for the purified extract was observed at milk CaCl2 concentration of 0.02 m and at milk pH of 5. These properties, except for temperature, were similar to those of rennet.
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The thermal stability of the enzyme with respect to its milk-clotting activity was greater with calf chymosin.
“The thermal stability of the enzyme with respect to its MCA was greater with calf chymosin (Nouani et al., 2009).”
From Invited review: Milk-clotting enzymes of microbial origin and their role in cheesemaking-A review. (2025) · cites this paper as Nouani et al., 2009
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Invited review: Milk-clotting enzymes of microbial origin and their role in cheesemaking-A review.
Wiśniewski, P., Małkowska-Kowalczyk, M., Łobacz, A., Zadernowska, A., & Żulewska, J. (2025) · Journal of dairy science · doi:10.3168/jds.2025-26973 · Cited by 10 · see citing works
- Authors
- NOUANI, A., BELHAMICHE, N., SLAMANI, R., BELBRAOUET, S., FAZOUANE, F., & BELLAL, M. M.
- Affiliations
- University of Boumerdes (DZ)
- École Nationale Supérieure Agronomique (DZ)
- Université de Moncton (CA)
- Corresponding author
- BELBRAOUET, S
- Venue
- International Journal of Dairy Technology
- Publisher
- Wiley
- Published
- 2009-01-14
- Volume
- 62
- Issue
- 1
- Pages
- 112-117
- ISSN
- 1364-727X (ISSN-L)
- 1471-0307
- OpenAlex
- W1964546056
- Cited by
- 26
- References
- 23
- Citations by year
- 2026: 1 · 2025: 1 · 2022: 1 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 4 · 2015: 4 · 2014: 2 · 2013: 2 · 2012: 2
- FWCI
- 1.63
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Dairy Technology (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Life Sciences › Agricultural and Biological Sciences › Food Science › Advanced Scientific Research Methods
- Keywords
- extracellular protease, Mucor pusillus, enzyme purification, milk coagulation, enzyme characterization, thermal stability, calcium chloride concentration, pH optimum, monomeric enzyme
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