The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
The human brain endothelial cell line hCMEC/D3 as a human blood‐brain barrier model for drug transport studies
Abstract
The human brain endothelial capillary cell line hCMEC/D3 has been developed recently as a model for the human blood‐brain barrier. In this study a further characterization of this model was performed with special emphasis on permeability properties and active drug transport. Para‐ or transcellular permeabilities (P e) of inulin (0.74 × 10−3 cm/min), sucrose (1.60 × 10−3 cm/min), lucifer yellow (1.33 × 10−3 cm/min), morphine (5.36 × 10−3 cm/min), propranolol (4.49 × 10−3 cm/min) and midazolam (5.13 × 10−3 cm/min) were measured. By addition of human serum the passive permeability of sucrose could be reduced significantly by up to 39%. Furthermore, the expression of a variety of drug transporters (ABCB1, ABCG2, ABCC1–5) as well as the human transferrin receptor was demonstrated on the mRNA level. ABCB1, ABCG2 and transferrin receptor proteins were detected and functional activity of ABCB1, ABCG2 and the ABCC family was quantified in efflux experiments. Furthermore, ABCB1‐mediated bidirectional transport of rhodamine 123 was studied. The transport rate from the apical to the basolateral compartment was significantly lower than that in the inverse direction, indicating directed p‐glycoprotein transport. The results of this study demonstrate the usefulness of the hCMEC/D3 cell line as an in vitro model to study drug transport at the level of the human blood‐brain barrier.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Astrocyte-endothelial interactions at the blood-brain barrier
Abbott, N. J., Rönnbäck, L., & Hansson, E. (2006) · Nature Reviews Neuroscience · doi:10.1038/nrn1824 · Cited by 5,595
Cordon-Cardo, C., O'Brien, J. P., Casals, D., Rittman-Grauer, L., Biedler, J. L., Melamed, M. R., & Bertino, J. R. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.2.695 · Cited by 1,750
Blood‐brain barrier‐specific properties of a human adult brain endothelial cell line
Weksler, B. B., Subileau, E. A., Perrière, N., Charneau, P., Holloway, K., Leveque, M., Tricoire‐Leignel, H., Nicotra, A., Bourdoulous, S., Turowski, P., et al. (2005) · The FASEB Journal · doi:10.1096/fj.04-3458fje · Cited by 1,330
Self-citationTight junctions of the blood–brain barrier
Wolburg, H., & Lippoldt, A. (2002) · Vascular Pharmacology · doi:10.1016/s1537-1891(02)00200-8 · Cited by 1,183
Schinkel, A. H., Wagenaar, E., van Deemter, L., Mol, C. A., & Borst, P. (1995) · Journal of Clinical Investigation · doi:10.1172/jci118214 · Cited by 1,107
P-Glycoprotein, a gatekeeper in the blood–brain barrier
Schinkel, A. H. (1999) · Advanced Drug Delivery Reviews · doi:10.1016/s0169-409x(98)00085-4 · Cited by 960
A cell culture model of the blood-brain barrier.
Rubin, L. L., Hall, D. E., Porter, S., Barbu, K., Cannon, C., Horner, H. C., Janatpour, M., Liaw, C. W., Manning, K., & Morales, J. (1991) · The Journal of cell biology · doi:10.1083/jcb.115.6.1725 · Cited by 765
Allen, J. D., van Loevezijn, A., Rademaker-Lakhai, J. M., van der Valk, M., van Tellingen, O., Reid, G., Schellens, J. H. M., Koomen, G., & Schinkel, A. H. (2002) · PubMed · Cited by 704
Role of drug efflux transporters in the brain for drug disposition and treatment of brain diseases
Löscher, W., & Potschka, H. (2005) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2005.04.006 · Cited by 617
Dombrowski, S. M., Desai, S. Y., Marroni, M., Cucullo, L., Goodrich, K., Bingaman, W., Mayberg, M. R., Bengez, L., & Janigro, D. (2001) · Epilepsia · doi:10.1046/j.1528-1157.2001.12301.x · Cited by 426
Cited by
Helms, H. C., Abbott, N. J., Burek, M., Cecchelli, R., Couraud, P.-O., Deli, M. A., Förster, C., Galla, H. J., Romero, I. A., Shusta, E. V., et al. (2016) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1177/0271678x16630991 · Cited by 843
Self-citationThe hCMEC/D3 cell line as a model of the human blood brain barrier
Weksler, B., Romero, I. A., & Couraud, P.-O. (2013) · Fluids and Barriers of the CNS · doi:10.1186/2045-8118-10-16 · Cited by 753
Self-citationGlucose Transporters at the Blood-Brain Barrier: Function, Regulation and Gateways for Drug Delivery
Patching, S. G. (2017) · Molecular Neurobiology · doi:10.1007/s12035-015-9672-6 · Cited by 361
Hatherell, K., Couraud, P.-O., Romero, I. A., Weksler, B., & Pilkington, G. J. (2011) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2011.05.012 · Cited by 334
Self-citationNeves, A. R., Queiroz, J. F., Weksler, B., Romero, I. A., Couraud, P.-O., & Reis, S. (2015) · Nanotechnology · doi:10.1088/0957-4484/26/49/495103 · Cited by 122
Self-citation
Metadata
- Authors
- Poller, B., Gutmann, H., Krähenbühl, S., Weksler, B., Romero, I., Couraud, P., Tuffin, G., Drewe, J., & Huwyler, J.
- Venue
- Journal of Neurochemistry
- Publisher
- Wiley
- Published
- 2008-11-11
- Volume
- 107
- Issue
- 5
- Pages
- 1358-1368
- ISSN
- 0022-3042 (ISSN-L)
- 1471-4159
- OpenAlex
- W2070902035
- Cited by
- 317
- References
- 46
- FWCI
- 6.93
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: The human brain endothelial cell line hCMEC/D3 as a human blood‐brain barrier model for drug transport studies. https://app.cochat.ai/papers/10.1111/j.1471-4159.2008.05730.x
- The paper
- Poller, B., Gutmann, H., Krähenbühl, S., Weksler, B., Romero, I., Couraud, P., Tuffin, G., Drewe, J., & Huwyler, J. (2008). The human brain endothelial cell line hCMEC/D3 as a human blood‐brain barrier model for drug transport studies. Journal of Neurochemistry. https://doi.org/10.1111/j.1471-4159.2008.05730.x
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
