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Simultaneous determination of volatile and non-volatile acidic fermentation products of anaerobes by capillary gas chromatography
Abstract
A method previously used for the analysis of organic acids in silage has been applied to the detection and quantification of acidic fermentation products (C1 to C6 volatile fatty acids, lactic and succinic acid) of rumen bacteria and anaerobic fungi grown in pure culture. The acids were converted to tertiary butyldimethylsilyl derivatives prior to separation on a 30 m DB1 capillary gas chromatographic column. The quantitative recoveries of formic and succinic acids were found to be comparable to the recoveries of other acids reported in the original study. The quantitative recovery of lactic acid was found to be dependent on storage of the samples at ambient temperature for at least 24 h following derivatization. The simultaneous determination of a wide range of volatile and non‐volatile acidic products is an important feature of this method.
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Cited by
Duncan, S. H., Belenguer, A., Holtrop, G., Johnstone, A. M., Flint, H. J., & Lobley, G. E. (2007) · Applied and Environmental Microbiology · doi:10.1128/aem.02340-06 · Cited by 941
Belenguer, A., Duncan, S. H., Calder, A. G., Holtrop, G., Louis, P., Lobley, G. E., & Flint, H. J. (2006) · Applied and Environmental Microbiology · doi:10.1128/aem.72.5.3593-3599.2006 · Cited by 856
Self-citationRamirez-Farias, C., Slezak, K., Fuller, Z., Duncan, A., Holtrop, G., & Louis, P. (2008) · British Journal of Nutrition · doi:10.1017/s0007114508019880 · Cited by 822
The role of pH in determining the species composition of the human colonic microbiota
Duncan, S. H., Louis, P., Thomson, J. M., & Flint, H. J. (2009) · Environmental Microbiology · doi:10.1111/j.1462-2920.2009.01931.x · Cited by 786
Walker, A. W., Duncan, S. H., McWilliam Leitch, E. C., Child, M. W., & Flint, H. J. (2005) · Applied and Environmental Microbiology · doi:10.1128/aem.71.7.3692-3700.2005 · Cited by 796
Metadata
- Authors
- Richardson, A. J., Calder, A. G., Stewart, C. S., & Smith, A.
- Venue
- Letters in Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 1989-07
- Volume
- 9
- Issue
- 1
- Pages
- 5-8
- ISSN
- 0266-8254 (ISSN-L)
- 1472-765X
- OpenAlex
- W1985574696
- Cited by
- 325
- References
- 0
- FWCI
- 0.54
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Simultaneous determination of volatile and non-volatile acidic fermentation products of anaerobes by capillary gas chromatography. https://app.cochat.ai/papers/10.1111/j.1472-765x.1989.tb00278.x
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- Richardson, A. J., Calder, A. G., Stewart, C. S., & Smith, A. (1989). Simultaneous determination of volatile and non-volatile acidic fermentation products of anaerobes by capillary gas chromatography. Letters in Applied Microbiology. https://doi.org/10.1111/j.1472-765x.1989.tb00278.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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