Quantitative analysis of the cellular inflammatory response against biofilm bacteria in chronic wounds
Open accessAbstract
Chronic wounds are an important problem worldwide. These wounds are characterized by a persistent inflammatory stage associated with excessive accumulation and elevated cell activity of neutrophils, suggesting that there must be a persistent stimulus that attracts and recruits neutrophils to the wound. One such stimulus might be the presence of bacterial biofilms in chronic wounds. In the present study, biopsy specimens from chronic venous leg ulcers were investigated for the detection of bacteria using peptide nucleic acid‐based fluorescence in situ hybridization (PNA‐FISH) and confocal laser scanning microscopy. The bacteria in the wounds were often situated in large aggregates. To obtain a measure of the cellular inflammatory response against the bacteria in the chronic wounds, the amount of neutrophils accumulated at the site of infection was evaluated through differential neutrophil counting on the tissue sections from wounds containing either Pseudomonas aeruginosa or Staphylococcus aureus. The P. aeruginosa‐containing wounds had significantly higher numbers of neutrophils accumulated compared with the S. aureus‐containing wounds. These results are discussed in relation to the hypothesis that the presence of P. aeruginosa biofilms in chronic wounds may be one of the main factors leading to a persistent inflammatory response and impaired wound healing.
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LasI-LasR and RhlI-RhlR have been shown to regulate the expression of virulence factors such as exoenzyme S (ExoS) and exotoxin A (ToxA) which can further induce apoptosis in macrophages and neutrophils.
“LasI-LasR and RhlI-RhlR have been shown to regulate the expression of virulence factors such as exoenzyme S (ExoS) and exotoxin A (ToxA) which can further induce apoptosis in macrophages and neutrophils [33], [34], [35], [36].”
From Interactions of Methicillin Resistant Staphylococcus aureus USA300 and Pseudomonas aeruginosa in Polymicrobial Wound Infection (2013) · cites this paper as [34]
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- Authors
- Fazli, M., Bjarnsholt, T., Kirketerp‐Møller, K., Jørgensen, A., Andersen, C. B., Givskov, M., & Tolker‐Nielsen, T.
- Affiliations
- University of Copenhagen (DK)
- Bispebjerg Hospital (DK)
- Copenhagen University Hospital (DK)
- Venue
- Wound Repair and Regeneration
- Publisher
- Wiley
- Published
- 2011-04-21
- Volume
- 19
- Issue
- 3
- Pages
- 387-391
- ISSN
- 1067-1927 (ISSN-L)
- 1524-475X
- OpenAlex
- W1673697235
- Cited by
- 155
- References
- 18
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 12 · 2023: 11 · 2022: 18 · 2021: 10 · 2020: 12 · 2019: 8 · 2018: 12 · 2017: 13 · 2016: 10 · 2015: 11 · 2014: 14 · 2013: 12 · 2012: 4
- FWCI
- 9.72
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Wound Repair and Regeneration (publishedVersion)
- PubMed (publishedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
- Funders
- Strategic Research Council
- Statens Serum Institut
- Topics
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetic Foot Ulcer Assessment and Management
- Keywords
- chronic wounds, bacterial biofilms, neutrophil accumulation, chronic inflammation, chronic venous leg ulcers, Pseudomonas aeruginosa, Staphylococcus aureus, PNA-FISH, confocal laser scanning microscopy, wound infection, delayed wound healing
- MeSH terms
- Aged, Aged, 80 and over, Chronic Disease, Female, Humans, Inflammation, Male, Middle Aged, Pseudomonas aeruginosa, Staphylococcus aureus, Varicose Ulcer, Wound Healing, Wound Infection, In Situ Hybridization, Fluorescence, Biofilms, Peptide Nucleic Acids, Neutrophil Infiltration
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