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Cited by 34Blood transfusion and managementHematopoietic Stem Cell TransplantationNeutropenia and Cancer Infections

Effective storage of granulocytes collected by centrifugation leukapheresis from donors stimulated with granulocyte–colony‐stimulating factor

Hubel, Kai, Rodger, Elin, Gaviria, J. Milton, Price, Thomas H., Dale, David C., Liles, W. Conrad·2005·Transfusion
DOI 10.1111/j.1537-2995.2005.00636.x
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Abstract

BACKGROUND: Donor stimulation with granulocyte–colony‐stimulating factor (G‐CSF) has increased the number of neutrophils (PMNs) that can be collected for granulocyte transfusion therapy. Clinical utility, however, has been limited by the inability to store functional PMNs ex vivo. This study was conducted to determine whether granulocyte products from G‐CSF–stimulated donors could be effectively stored at reduced temperature (22°C vs. 10°C) with maintenance of functional properties in vitro and in vivo. STUDY DESIGN AND METHODS: Nine normal subjects received G‐CSF (600 µg subcutaneously) 12 hours before centrifugation leukapheresis. Granulocyte products were divided and stored for 24 and 48 hours under four conditions: 1) 22°C; 2) 22°C, with supplemental G‐CSF (100 ng/mL); 3) 10°C; and 4) 10°C, with supplemental G‐CSF. Functional PMN activity during ex vivo storage was assessed in vitro and in vivo by the skin‐window technique for granulocytes stored at 10°C for 24 hours. RESULTS: Surface expression of CD11b/CD18, CD14, CD16, CD32, and CD64 was maintained during 48‐hour storage at reduced temperature. Inducible respiratory burst activity, bactericidal activity, and fungicidal activity were preserved during storage for 48‐hour storage at 10°C. Proinflammatory cytokine production was decreased in product stored at 10°C. Supplemental G‐CSF ex vivo did not substantially improve functional activity during storage. After storage at 10°C for 24 hours, in vitro chemotactic potential was maintained, and transfused granulocytes retained capacity to circulate and migrate appropriately in vivo. CONCLUSIONS: Granulocyte product collected by centrifugation leukapheresis from G‐CSF–stimulated donors can be effectively stored at subphysiologic temperature for 24 hours with preservation of functional activity. Storage at 10°C appears to be slightly superior to storage at 22°C.

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Crossref · 10.1111/j.1537-2995.2005.00636.x

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  • Oral PMN counts are an established measure of functional activity; oral neutrophil counts measure PMN functional status as they reflect the ability of PMNs to transmigrate from the circulation to the mucosal surface.

    “Oral PMN counts are an established measure of functional activity; oral neutrophil counts measure PMN functional status as they reflect the ability of PMNs to transmigrate from the circulation to the mucosal surface [40-43].”

    From Oral neutrophils are an independent marker of the systemic inflammatory response after cardiac bypass (2014) · cites this paper as [42]

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Authors
Hubel, K., Rodger, E., Gaviria, J. M., Price, T. H., Dale, D. C., & Liles, W. C.
Affiliations
University of Washington (US)
Corresponding author
Liles, W. Conrad
ORCID
Venue
Transfusion
Publisher
Wiley
Published
2005-10-10
Volume
45
Issue
12
Pages
1876-1889
ISSN
  • 0041-1132 (ISSN-L)
  • 1537-2995
DOI
10.1111/j.1537-2995.2005.00636.x
OpenAlex
W1994208678
Cited by
34
References
75
Citations by year
2026: 2 · 2025: 1 · 2024: 1 · 2022: 2 · 2021: 2 · 2018: 2 · 2017: 3 · 2016: 1 · 2014: 1 · 2013: 4 · 2012: 2
FWCI
1.13
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Transfusion (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01hl053515-03)
  • National Heart, Lung, and Blood Institute (HL-53515)
Topics
  • Health Sciences › Medicine › Biochemistry › Blood transfusion and management
  • Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
  • Health Sciences › Medicine › Oncology › Neutropenia and Cancer Infections
Keywords
granulocyte colony-stimulating factor, neutrophils, granulocyte transfusion, cold storage, storage temperature, leukocyte function, respiratory burst, bactericidal activity, fungicidal activity, inflammatory cytokines, chemotaxis, skin window technique, CD11b/CD18, CD64
MeSH terms
Adult, Bacteria, Blood Donors, Blood Preservation, Cell Movement, Centrifugation, Female, Fungi, Granulocytes, Humans, Indicators and Reagents, Injections, Subcutaneous, Leukapheresis, Leukocyte Count, Luminol, Male, Middle Aged, Neutrophils, Cryopreservation, Granulocyte Colony-Stimulating Factor, Cytokines, Respiratory Burst, Leukocyte Transfusion

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