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Cited by 124Hematopoietic Stem Cell TransplantationBlood transfusion and managementNeutropenia and Cancer Infections

Neutrophil transfusions: kinetics and functions of neutrophils mobilized with granulocyte‐colony‐stimulating factor and dexamethasone

Dale, D.C., Liles, W.C., Llewellyn, C., Rodger, E., Price, T.H.·1998·Transfusion
DOI 10.1046/j.1537-2995.1998.38898375509.x
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Abstract

BACKGROUND: The collection of adequate numbers of neutrophils (polymorphonuclear leukocytes, PMNs) from normal donors has long hampered the development of neutrophil transfusion therapy. The stimulation of donors with granulocyte‐colony‐stimulating factor (G‐ CSF) plus dexamethasone is a promising way of improving PMN collections. STUDY DESIGN AND METHODS:Sixteen normal subjects received G‐CSF (600 micrograms subcutaneously) and dexamethasone (8 mg by mouth) 12 hours before leukapheresis. Measurements included PMN morphology, immunophenotype analysis, chemiluminescence, bactericidal activity, in vivo kinetics, and adverse effects. RESULTS:A mean of 77.4 +/− 6.4 × 10(9) PMNs was collected with each leukapheresis; 14 percent were bands. PMNs had increased surface expression of CD11b, CD18, CD14, CD32, and CD64. Bactericidal capacity against Staphylococcus aureus was normal. Inducible respiratory burst was maintained, although the responses to some agonists were diminished. Returned leukapheresis cells labeled with 3H‐diisopropylfluorophosphate had a modestly decreased percentage of recovery and circulated with a prolonged half‐ life. Migration of these cells to skin chambers was approximately equal to that of the subjects' own blood PMNs. Adverse effects included transient bone pain, headache, hunger, and insomnia. CONCLUSIONS:Precollection treatment of leukapheresis donors with G‐CSF plus dexamethasone is an effective way to enhance the collection of PMNs with normal or near‐normal functional properties for PMN transfusion therapy.

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  • Oral PMN counts are an established measure of functional activity; oral neutrophil counts measure PMN functional status as they reflect the ability of PMNs to transmigrate from the circulation to the mucosal surface.

    “Oral PMN counts are an established measure of functional activity; oral neutrophil counts measure PMN functional status as they reflect the ability of PMNs to transmigrate from the circulation to the mucosal surface [40-43].”

    From Oral neutrophils are an independent marker of the systemic inflammatory response after cardiac bypass (2014) · cites this paper as [41]

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Authors
Dale, D. C., Liles, W. C., Llewellyn, C., Rodger, E., & Price, T. H.
Affiliations
University of Washington (US)
Corresponding author
Dale, D.C.
ORCID
Dale, D.C.
Venue
Transfusion
Publisher
Wiley
Published
1998-08
Volume
38
Issue
8
Pages
713-721
ISSN
  • 0041-1132 (ISSN-L)
  • 1537-2995
DOI
10.1046/j.1537-2995.1998.38898375509.x
OpenAlex
W2093230583
Cited by
124
References
41
Citations by year
2025: 2 · 2024: 2 · 2022: 3 · 2021: 4 · 2019: 2 · 2018: 1 · 2017: 3 · 2016: 11 · 2015: 8 · 2014: 4 · 2013: 5 · 2012: 5
FWCI
3.98
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 1671 d)
Locations
  • Transfusion (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01hl053515-03)
  • National Heart, Lung, and Blood Institute (HL53515-02)
Topics
  • Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
  • Health Sciences › Medicine › Biochemistry › Blood transfusion and management
  • Health Sciences › Medicine › Oncology › Neutropenia and Cancer Infections
Keywords
neutrophil transfusion, granulocyte colony-stimulating factor, dexamethasone, leukapheresis, donor mobilization, neutrophil kinetics, neutrophil migration, bactericidal activity, respiratory burst, CD11b, CD64, Staphylococcus aureus, adverse effects
MeSH terms
Adult, Cell Movement, Dexamethasone, Female, Humans, Leukapheresis, Leukocyte Count, Luminescent Measurements, Male, Middle Aged, Neutrophils, Staphylococcus aureus, Antigens, CD, Immunophenotyping, Granulocyte Colony-Stimulating Factor, Respiratory Burst, Leukocyte Transfusion, L-Selectin

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