Batch and continuous culture-based selection strategies for acetic acid tolerance in xylose-fermenting Saccharomyces cerevisiae
Open accessAbstract
Acetic acid tolerance of Saccharomyces cerevisiae is crucial for the production of bioethanol and other bulk chemicals from lignocellulosic plant-biomass hydrolysates, especially at a low pH. This study explores two evolutionary engineering strategies for the improvement of acetic acid tolerance of the xylose-fermenting S. cerevisiae RWB218, whose anaerobic growth on xylose at pH 4 is inhibited at acetic acid concentrations >1 g L(-1) : (1) sequential anaerobic, batch cultivation (pH 4) at increasing acetic acid concentrations and (2) prolonged anaerobic continuous cultivation without pH control, in which acidification by ammonium assimilation generates selective pressure for acetic acid tolerance. After c. 400 generations, the sequential-batch and continuous selection cultures grew on xylose at pH≤4 with 6 and 5 g L(-1) acetic acid, respectively. In the continuous cultures, the specific xylose-consumption rate had increased by 75% to 1.7 g xylose g(-1) biomass h(-1) . After storage of samples from both selection experiments at -80 °C and cultivation without acetic acid, they failed to grow on xylose at pH 4 in the presence of 5 g L(-1) acetic acid. Characterization in chemostat cultures with linear acetic acid gradients demonstrated an acetate-inducible acetic acid tolerance in samples from the continuous selection protocol.
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Mutational robustness tends to increase with mutation rate and decline with population size.
“Furthermore, it was shown that mutational robustness tends to increase with mutation rate and decline with population size [40].”
From Adaptive laboratory evolution—Principles and applications for biotechnology (2013) · cites this paper as [40]
In silico approaches are widely applied in order to address evolutionary questions.
“Similarly, in silico approaches are widely applied in order to address evolutionary questions [40,172].”
From Adaptive laboratory evolution—Principles and applications for biotechnology (2013) · cites this paper as [40]
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Adaptive laboratory evolution—Principles and applications for biotechnology
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- Authors
- Wright, J., Bellissimi, E., de Hulster, E., Wagner, A., Pronk, J. T., & van Maris, A. J. A.
- Affiliations
- SIB Swiss Institute of Bioinformatics (CH)
- Delft University of Technology (NL)
- Venue
- FEMS Yeast Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-02-14
- Volume
- 11
- Issue
- 3
- Pages
- 299-306
- ISSN
- 1567-1356 (ISSN-L)
- OpenAlex
- W2155489855
- Cited by
- 98
- References
- 28
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 4 · 2023: 3 · 2022: 4 · 2021: 4 · 2020: 9 · 2019: 7 · 2018: 8 · 2017: 7 · 2016: 9 · 2015: 4 · 2014: 10 · 2013: 11 · 2012: 10
- FWCI
- 4.67
- Type
- journal article
- Language
- en
- Open access
- green
- www.zora.uzh.ch
- Licence
- other-oa
- Locations
- FEMS Yeast Research (publishedVersion)
- PubMed (publishedVersion)
- Zurich Open Repository and Archive (University of Zurich) (acceptedVersion, other-oa)
- Zurich Open Repository and Archive (University of Zurich) (submittedVersion, other-oa)
- Zurich Open Repository and Archive (University of Zurich)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- acetic acid tolerance, Saccharomyces cerevisiae, xylose fermentation, evolutionary engineering, batch selection, continuous culture, lignocellulosic hydrolysates, low pH, ammonium assimilation, bioethanol production, chemostat
- MeSH terms
- Ethanol, Culture Media, Fermentation, Hydrogen-Ion Concentration, Saccharomyces cerevisiae, Xylose, Biomass, Acetic Acid
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