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Isolation and survey of novel fluoroacetate-degrading bacteria belonging to the phylum Synergistetes

Davis, Carl K., Webb, Richard I., Sly, Lindsay I., Denman, Stuart E., McSweeney, Chris S.·2012·FEMS Microbiology Ecology
DOI 10.1111/j.1574-6941.2012.01338.x
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Abstract

Microbial dehalogenation of chlorinated compounds in anaerobic environments is well known, but the degradation of fluorinated compounds under similar conditions has rarely been described. Here, we report on the isolation of a bovine rumen bacterium that metabolizes fluoroacetate under anaerobic conditions, the mode of degradation and its presence in gut ecosystems. The bacterium was identified using 16S rRNA gene sequence analysis as belonging to the phylum Synergistetes and was designated strain MFA1. Growth was stimulated by amino acids with greater quantities of amino acids metabolized in the presence of fluoroacetate, but sugars were not fermented. Acetate, formate, propionate, isobutryate, isovalerate, ornithine and H(2) were end products of amino acid metabolism. Acetate was the primary end product of fluoroacetate dehalogenation, and the amount produced correlated with the stoichiometric release of fluoride which was confirmed using fluorine nuclear magnetic resonance ((19) F NMR) spectroscopy. Hydrogen and formate produced in situ were consumed during dehalogenation. The growth characteristics of strain MFA1 indicated that the bacterium may gain energy via reductive dehalogenation. This is the first study to identify a bacterium that can anaerobically dehalogenate fluoroacetate. Nested 16S rRNA gene-specific PCR assays detected the bacterium at low numbers in the gut of several herbivore species.

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Metadata

Authors
Davis, C. K., Webb, R. I., Sly, L. I., Denman, S. E., & McSweeney, C. S.
Affiliations
  • Centre for Microscopy and Microanalysis; University of Queensland; St Lucia; Qld; Australia
  • School of Chemistry and Molecular Biosciences; University of Queensland; St Lucia; Qld; Australia
  • CSIRO Livestock Industries; Queensland Bioscience Precinct; St Lucia; Qld; Australia
Venue
FEMS Microbiology Ecology
Publisher
Oxford University Press (OUP)
Published
2012-03-20
Volume
80
Issue
3
Pages
671-684
ISSN
0168-6496
DOI
10.1111/j.1574-6941.2012.01338.x
Cited by
61
References
0
Language
en

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