Nucleotide sequence and distribution of gene tetO encoding tetracycline resistance in Campylobacter coli
Open accessAbstract
The nucleotide sequence of the tetracycline resistance gene encoded by the self-transferable plasmid pIP1433 in Campylobacter coli BM2509 has been determined. The resistance gene, tetO, was located in an open reading frame of 1917 bp corresponding to a protein with a calculated Mr of 72 290. The transcriptional and translational signals have been identified. The tetO gene exhibits 76% sequence identity with tetM from Streptococcus and the deduced amino acid sequences are 77% homologous. The substitutions are scattered throughout indicating that the two genes have diverged from a common ancestor. We suggest that tetracycline resistance in Campylobacter is due to acquisition of a gene originating in a Gram-positive bacterium. The distribution of tetO in hundred strains of C. jejuni and C. coli resistant to tetracycline (MIC ≥ 32 μg/ml) and to minocycline (MIC ≥ 4 μg/ml) was studied by dot blot hybridization under high stringency conditions using a 1.4-kb intragenic probe. Homology with tetO was detected in 98 strains isolated from humans and animals in various geographical areas. None of the strains hybridized with tetM under the same conditions. These data indicate that tetO is widespread among Campylobacter spp. probably due to conjugative plasmids.
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Tet(O), like other RPPs, seems to have originated in Streptomyces rimosus, which harbors otrA, an RPP determinant.
“However, Tet(O), like the other RPPs, seems to have originated in the natural producer of oxytetracycline, Streptomyces rimosus , which harbors otrA , an RPP determinant (16, 44, 49).”
From Ribosomal Protection Proteins and Their Mechanism ofTetracyclineResistance (2003) · cites this paper as [44]
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Ribosomal Protection Proteins and Their Mechanism ofTetracyclineResistance
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- Authors
- Sougakoff, W., Papadopoulou, B., Nordmann, P., & Courvalin, P.
- Affiliations
- Institut Pasteur (FR)
- Corresponding author
- Sougakoff, Wladimir
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 1987-09
- Volume
- 44
- Issue
- 1
- Pages
- 153-159
- ISSN
- 0378-1097 (ISSN-L)
- 1574-6968
- OpenAlex
- W2093068663
- Cited by
- 124
- References
- 29
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 2 · 2023: 5 · 2022: 1 · 2021: 3 · 2020: 3 · 2019: 1 · 2018: 3 · 2016: 2 · 2015: 1 · 2014: 3 · 2012: 2
- FWCI
- 18.18
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEMS Microbiology Letters (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Salmonella and Campylobacter epidemiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Campylobacter coli, Campylobacter jejuni, tetracycline resistance, tetM, conjugative plasmids, gene distribution, horizontal gene transfer, minocycline resistance, dot blot
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