Cited by 17RNA and protein synthesis mechanismsBacterial Genetics and BiotechnologyAntibiotic Resistance in Bacteria

Tet(O), a protein that mediates ribosomal protection to tetracycline, binds, and hydrolyses GTP

Taylor, Diane E., Jerome, Lori J., Grewal, Jaswinder, Chang, Nicholas·1995·Canadian Journal of Microbiology

Abstract

The tet(O) tetracycline resistance gene, originally cloned from Campylobacter jejuni, mediates resistance by ribosomal protection. Using partially purified Tet(O) protein of 68 000 Da whose identity was verified by ribosomal protection assays, amino terminal sequencing, and immunoblotting using an antibody raised against the deduced 15 amino acids at the carboxyl terminus of the Tet(O) protein, the Tet(O) protein was found to bind to [α-32 P]GTP and [3 H]GDP using a filter binding assay. [γ-32 P]GTP hydrolysis by Tet(O) was also demonstrated and was found to be time dependent with more than 50% of the hydrolysis activity occurring within the first 5 min. The GTPase activity of Tet(O) appears to be ribosome dependent, suggesting that ribosomes act as an effector similar to other G proteins involved in signal transduction.Key words: ribosomes, tetracycline resistance, GTPase, protein synthesis.

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Crossref · 10.1139/m95-134

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  • RPPs bind and hydrolyze GTP in a ribosome-dependent manner, and maintenance of this activity is important for in vivo activity.

    “Accordingly, the RPPs bind and hydrolyze GTP in a ribosome-dependent manner (5, 6, 53), and maintenance of this activity is important for in vivo activity (11, 23).”

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Authors
Taylor, D. E., Jerome, L. J., Grewal, J., & Chang, N.
Venue
Canadian Journal of Microbiology
Published
1995-11-01
Volume
41
Issue
11
Pages
965-970
ISSN
  • 0008-4166 (ISSN-L)
  • 1480-3275
OpenAlex
W1992482291
Cited by
17
References
32
Citations by year
2025: 1 · 2019: 2 · 2014: 2
FWCI
0.74
Type
journal article
Language
en
Open access
closed
Locations
Canadian Journal of Microbiology (publishedVersion)
Funders
Pfizer
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
Keywords
tetracycline resistance, GTPase activity, GTP hydrolysis, GTP binding, Campylobacter jejuni, protein synthesis

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