Tet(O), a protein that mediates ribosomal protection to tetracycline, binds, and hydrolyses GTP
Abstract
The tet(O) tetracycline resistance gene, originally cloned from Campylobacter jejuni, mediates resistance by ribosomal protection. Using partially purified Tet(O) protein of 68 000 Da whose identity was verified by ribosomal protection assays, amino terminal sequencing, and immunoblotting using an antibody raised against the deduced 15 amino acids at the carboxyl terminus of the Tet(O) protein, the Tet(O) protein was found to bind to [α-32 P]GTP and [3 H]GDP using a filter binding assay. [γ-32 P]GTP hydrolysis by Tet(O) was also demonstrated and was found to be time dependent with more than 50% of the hydrolysis activity occurring within the first 5 min. The GTPase activity of Tet(O) appears to be ribosome dependent, suggesting that ribosomes act as an effector similar to other G proteins involved in signal transduction.Key words: ribosomes, tetracycline resistance, GTPase, protein synthesis.
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RPPs bind and hydrolyze GTP in a ribosome-dependent manner, and maintenance of this activity is important for in vivo activity.
“Accordingly, the RPPs bind and hydrolyze GTP in a ribosome-dependent manner (5, 6, 53), and maintenance of this activity is important for in vivo activity (11, 23).”
From Ribosomal Protection Proteins and Their Mechanism ofTetracyclineResistance (2003) · cites this paper as [53]
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- Authors
- Taylor, D. E., Jerome, L. J., Grewal, J., & Chang, N.
- ORCID
- Taylor, Diane E.
- Venue
- Canadian Journal of Microbiology
- Publisher
- Canadian Science Publishing
- Published
- 1995-11-01
- Volume
- 41
- Issue
- 11
- Pages
- 965-970
- ISSN
- 0008-4166 (ISSN-L)
- 1480-3275
- OpenAlex
- W1992482291
- Cited by
- 17
- References
- 32
- Citations by year
- 2025: 1 · 2019: 2 · 2014: 2
- FWCI
- 0.74
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Canadian Journal of Microbiology (publishedVersion)
- Funders
- Pfizer
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
- Keywords
- tetracycline resistance, GTPase activity, GTP hydrolysis, GTP binding, Campylobacter jejuni, protein synthesis
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