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Gene replacement in Staphylococcus carnosus and Staphylococcus xylosus
Abstract
A system for high-efficiency gene replacement in Staphylococcus carnosus and Staphylococcus xylosus has been developed, that is based on temperature-sensitive Escherichia coli-Staphylococcus shuttle vectors for fragment delivery and erythromycin resistance cassettes to facilitate selection of genomic copies of disrupted genes. The approach was tested by constructing a phosphotransferase-deficient mutant of S. carnosus and an S. xylosus mutant strain unable to utilize sucrose. Allelic replacements were observed at rather high frequencies, ranging from approximately 10% for the ptsI gene in S. carnosus up to 50% for the scrB gene in S. xylosus. These differences most likely reflect the length of homology rather than strain-specific variations in recombination efficiencies. Apart from the staphylococcal species tested in this study, the system appears to be applicable in other staphylococci.
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Patel, A. H., Nowlan, P., Weavers, E. D., & Foster, T. (1987) · Infection and Immunity · doi:10.1128/iai.55.12.3103-3110.1987 · Cited by 280
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Peschel, A., Jack, R. W., Otto, M., Collins, L. V., Staubitz, P., Nicholson, G., Kalbacher, H., Nieuwenhuizen, W. F., Jung, G., Tarkowski, A., et al. (2001) · The Journal of Experimental Medicine · doi:10.1084/jem.193.9.1067 · Cited by 786
Vuong, C., Kocianova, S., Voyich, J. M., Yao, Y., Fischer, E. R., DeLeo, F. R., & Otto, M. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m411374200 · Cited by 592
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Metadata
- Authors
- Brückner, R.
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-01-17
- Volume
- 151
- Issue
- 1
- Pages
- 1-8
- ISSN
- 0378-1097 (ISSN-L)
- OpenAlex
- W1990046762
- Cited by
- 294
- References
- 1
- FWCI
- 10.60
- Type
- journal article
- Language
- en
- Open access
- closed
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- Brückner, R. (2006). Gene replacement in Staphylococcus carnosus and Staphylococcus xylosus. FEMS Microbiology Letters. https://doi.org/10.1111/j.1574-6968.1997.tb10387.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.