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Cited by 294

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Gene replacement in Staphylococcus carnosus and Staphylococcus xylosus

Brückner, Reinhold·2006·FEMS Microbiology Letters

Abstract

A system for high-efficiency gene replacement in Staphylococcus carnosus and Staphylococcus xylosus has been developed, that is based on temperature-sensitive Escherichia coli-Staphylococcus shuttle vectors for fragment delivery and erythromycin resistance cassettes to facilitate selection of genomic copies of disrupted genes. The approach was tested by constructing a phosphotransferase-deficient mutant of S. carnosus and an S. xylosus mutant strain unable to utilize sucrose. Allelic replacements were observed at rather high frequencies, ranging from approximately 10% for the ptsI gene in S. carnosus up to 50% for the scrB gene in S. xylosus. These differences most likely reflect the length of homology rather than strain-specific variations in recombination efficiencies. Apart from the staphylococcal species tested in this study, the system appears to be applicable in other staphylococci.

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Crossref · 10.1111/j.1574-6968.1997.tb10387.x

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Metadata

Authors
Brückner, R.
Venue
FEMS Microbiology Letters
Published
2006-01-17
Volume
151
Issue
1
Pages
1-8
ISSN
0378-1097 (ISSN-L)
OpenAlex
W1990046762
Cited by
294
References
1
FWCI
10.60
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Gene replacement in Staphylococcus carnosus and Staphylococcus xylosus. https://app.cochat.ai/papers/10.1111/j.1574-6968.1997.tb10387.x
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Brückner, R. (2006). Gene replacement in Staphylococcus carnosus and Staphylococcus xylosus. FEMS Microbiology Letters. https://doi.org/10.1111/j.1574-6968.1997.tb10387.x

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