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Cited by 94Protist diversity and phylogenyMarine and coastal plant biologyGenomics and Phylogenetic Studies

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Arthrospira(‘Spirulina ’) strains from four continents are resolved into only two clusters, based on amplified ribosomal DNA restriction analysis of the internally transcribed spacer

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Scheldeman, Patsy, Baurain, Denis, Bouhy, Rachel, Scott, Mark, Mühling, Martin, Whitton, Brian A, Belay, Amha, Wilmotte, Annick·1999·FEMS Microbiology Letters
DOI 10.1111/j.1574-6968.1999.tb13471.x
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Abstract

We present the results of a phylogenetic study, based on amplified ribosomal DNA restriction analysis of the rDNA operon, of 37 Arthrospira ('Spirulina') cultivated clonal strains from four continents. In addition, duplicates from different culture collections or markedly different morphotypes of particular strains established as clonal cultures were treated as separate entries, resulting in a total of 51 tested cultures. The strain Spirulina laxissima SAG 256.80 was included as outgroup. The 16S rRNA genes appeared too conserved for discrimination of the strains by amplified ribosomal DNA restriction analysis, and thus the internally transcribed spacer was selected as molecular taxonomic marker. The internally transcribed spacer sequences situated between the 16S and the 23S rRNA were amplified by polymerase chain reaction and yielded amplicons of about 540 bp. Direct use of cells for polymerase chain reaction seemed to inhibit the amplification reaction. This was overcome by the design of a crude lysis protocol and addition of bovine serum albumin in the polymerase chain reaction mix. The amplicons were digested with four restriction enzymes (EcoRV, Hhal, Hinfl, Msel) and the banding patterns obtained were analyzed. Cluster analysis showed the separation of all the strains into two main clusters. No clear relationships could be observed between this division into two clusters and the geographic origin of the strains, or their designation in the culture collections, or their morphology.

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Crossref · 10.1111/j.1574-6968.1999.tb13471.x

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  • The clades may correspond to two previously reported clusters determined via rDNA restriction analysis and phylogenetic analysis using 16S rRNA and cpcBA-IGS sequences.

    “These clades may correspond to two previously reported clusters determined via rDNA restriction analysis 20 and phylogenetic analysis using the 16S rRNA and cpcBA-IGS sequences 21.”

    From The Draft Genome of a Hydrogen-producing Cyanobacterium, Arthrospira platensis NIES-46 (2019) · cites this paper as [20]

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Research works printed but not linked: 17

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  3. 3 Tomaselli, L. (1997) Morphology, ultrastructure and taxonomy. In: Spirulina platensis (Arthrospira): Physiology, Cell-Biology and Biotechnology (Vonshak, A., Ed.), pp. 1–15. Taylor and Francis, London, UK.
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  12. 15 Heery, D.M., Gannon, F. and Powell, R. (1990) A simple method for subcloning DNA fragments from gel slices. Trends Genet. 6, 173. · DOI
  13. Viti, 1997, Genotypic diversity and typing of cyanobacterial strains of the genus Arthrospira by very sensitive total DNA restriction profile analysis, Res. Microbiol., 148, 605, 10.1016/S0923-2508(97)88084-9 · DOI
  14. Mullins, 1995, Genetic comparisons reveal the same unknown bacterial lineages in Atlantic and Pacific bacterioplankton communities, Limnol. Oceanogr., 40, 148, 10.4319/lo.1995.40.1.0148 · DOI
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Metadata

Authors
Scheldeman, P., Baurain, D., Bouhy, R., Scott, M., Mühling, M., Whitton, B. A., Belay, A., & Wilmotte, A.
Affiliations
  • University of Liège (BE)
  • Durham University (GB)
Corresponding author
Wilmotte, Annick
ORCID
Venue
FEMS Microbiology Letters
Publisher
Oxford University Press (OUP)
Published
1999-03
Volume
172
Issue
2
Pages
213-222
ISSN
  • 0378-1097 (ISSN-L)
  • 1574-6968
DOI
10.1111/j.1574-6968.1999.tb13471.x
OpenAlex
W2165329950
Cited by
94
References
2
Citations by year
2026: 3 · 2025: 2 · 2024: 5 · 2023: 2 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 2 · 2018: 3 · 2017: 2 · 2016: 4 · 2015: 3 · 2014: 2 · 2013: 3 · 2012: 7
FWCI
0.91
Type
journal article
Language
en
Open access
Locations
  • FEMS Microbiology Letters (publishedVersion)
  • PubMed (publishedVersion)
  • Ghent University Academic Bibliography (Ghent University) (submittedVersion)
  • ORBi (University of Liège) (submittedVersion, other-oa)
Funders
Università degli Studi di Pavia
Topics
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Protist diversity and phylogeny
  • Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal plant biology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
Keywords
Arthrospira, Spirulina, phylogenetic clustering, strain diversity, PCR inhibition, restriction fragment patterns
MeSH terms
Cyanobacteria, DNA, Bacterial, DNA, Ribosomal, Phylogeny, RNA, Ribosomal, 16S, RNA, Ribosomal, 23S, Polymerase Chain Reaction

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