The iron-regulated surface proteins IsdA, IsdB, and IsdH are not required for heme iron utilization in Staphylococcus aureus
Abstract
The iron-regulated surface determinant proteins (Isd) of Staphylococcus aureus are expressed during iron limitation and have been proposed to be involved in the scavenging of iron from heme. In this study, the genes encoding the surface proteins IsdA, IsdB, and IsdH were inactivated in order to determine their combined role. The triple mutant was found to have no defect in growth under any conditions of iron limitation tested. Also using a mouse septic arthritis model of S. aureus systemic disease, no significant difference in bacterial load was observed for the triple mutant, compared with its otherwise isogenic parent.
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Two recent reports challenged the large literature on the Isd system's role in hemoglobin/heme-derived iron acquisition.
“However, the wealth of literature on the contribution of the Isd system to hemoglobin/heme-derived iron acquisition was challenged by 2 recent reports [36, 37].”
From IsdB-dependent hemoglobin binding is required for acquisition of heme by Staphylococcus aureus (2014) · cites this paper as [36]
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IsdB-dependent hemoglobin binding is required for acquisition of heme by Staphylococcus aureus
Pishchany, G., Sheldon, J. R., Dickson, C. F., Alam, M. T., Read, T. D., Gell, D. A., Heinrichs, D. E., & Skaar, E. P. (2014) · The Journal of Infectious Diseases · doi:10.1093/infdis/jit817 · Cited by 88 · see citing works
- Authors
- Hurd, A. F., Garcia-Lara, J., Rauter, Y., Cartron, M., Mohamed, R., & Foster, S. J.
- Affiliations
- University of Sheffield (GB)
- Krebs Institute, Department of Molecular Biology and Biotechnology; University of Sheffield; Sheffield; UK
- Corresponding author
- Foster, Simon J.
- ORCID
- Foster, Simon J.
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 2012-02-09
- Volume
- 329
- Issue
- 1
- Pages
- 93-100
- ISSN
- 0378-1097 (ISSN-L)
- OpenAlex
- W2077486832
- Cited by
- 20
- References
- 42
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 2 · 2014: 1 · 2013: 5 · 2012: 1
- FWCI
- 1.99
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEMS Microbiology Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Keywords
- ISDA, ISDB, IsdH, Staphylococcus aureus, iron limitation, iron acquisition, bacterial virulence, septic arthritis, gene inactivation
- MeSH terms
- Animals, Antigens, Bacterial, Arthritis, Infectious, Disease Models, Animal, Heme, Iron, Membrane Proteins, Receptors, Cell Surface, Staphylococcal Infections, Staphylococcus aureus, Gene Expression Regulation, Bacterial, Cation Transport Proteins, Mice, Gene Knockout Techniques, Bacterial Load
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