IsdA and IsdB antibodies protect mice against Staphylococcus aureus abscess formation and lethal challenge

Kim, Hwan Keun; DeDent, Andrea; Cheng, Alice G.; McAdow, Molly; Bagnoli, Fabio; Missiakas, Dominique M.; Schneewind, Olaf

doi:10.1016/j.vaccine.2010.02.097

Cited by 158Antimicrobial Resistance in StaphylococcusBiochemical and Structural Characterizationvaccines and immunoinformatics approaches

IsdA and IsdB antibodies protect mice against Staphylococcus aureus abscess formation and lethal challenge

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Kim, Hwan Keun, DeDent, Andrea, Cheng, Alice G., McAdow, Molly, Bagnoli, Fabio, Missiakas, Dominique M., Schneewind, Olaf·2010·Vaccine

DOI 10.1016/j.vaccine.2010.02.097PMC PMC3095377

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Abstract

Staphylococcus aureus is the most frequent cause of bacteremia and hospital-acquired infection, however a vaccine that prevents staphylococcal disease is currently not available. Two sortase-anchored surface proteins, IsdA and IsdB, have been identified as subunit vaccines that, following active immunization, protect experimental animals against intravenous challenge with staphylococci. Here we investigate the molecular basis of this immunity and report that, when passively transferred to naïve mice, purified antibodies directed against IsdA or IsdB protected against staphylococcal abscess formation and lethal intravenous challenge. When added to mouse blood, IsdA- or IsdB-specific antibodies did not promote rapid opsonophagocytic killing of wild-type staphylococci. Antibodies directed against IsdA interfered with heme-binding and IsdB antibodies perturbed the ability of this surface protein to bind hemoglobin. As the structural genes for isdA and isdB are required for heme-iron scavenging during the pathogenesis of infection, we hypothesize that IsdA and IsdB antibodies may at least in part provide protection against staphylococci by interfering with the pathogen's heme-iron scavenging mechanisms.

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Examples include screening strategies to identify small-molecule inhibitors of FeoB, or antibodies that target IsdA and IsdB and prevent S. aureus from binding to haem, which has led to protection in mouse models of infection.
“Examples include screening strategies to identify small- molecule inhibitors of FeoB 183-185 , or antibodies that target IsdA and IsdB and prevent S. aureus from binding to haem, which has led to protection in mouse models of infection 186,187 .”
From Nutritional immunity: the battle for nutrient metals at the host–pathogen interface (2022) · cites this paper as [187]
The Abstract reports that antibodies against IsdA or IsdB protected mice from abscess formation and lethal challenge. Results §3.5 distinguishes their effects: anti-IsdA interfered with heme binding, while anti-IsdB blocked hemoglobin binding but did not reduce heme binding. The Discussion describes interference with heme-iron scavenging as a plausible, still speculative explanation for protection, not an established cause. Page numbers were not supplied. (LLM, conf=0.89)
Experimental — accuracy not yet measured
IsdA and IsdB were identified as vaccine candidates using reverse vaccinology, and immunization with these proteins is protective in murine models of infection.
“IsdA and IsdB were identified as vaccine candidates using “reverse vaccinology” [119], and subsequent studies confirmed that immunization with these proteins is protective in murine models of infection [117, 120–123].”
From Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity (2012) · cites this paper as [117]
Mutation of isdA, isdB, and isdC, but not isdH, decreases bacterial recovery and organ abscesses in murine models of systemic infection.
“Mutation of isdA, isdB, and isdC, but not isdH, decreases bacterial recovery and organ abscesses in murine models of systemic infection [96, 116, 117].”
From Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity (2012) · cites this paper as [117]
Disruption of the Isd heme acquisition system at each step leads to decreased virulence in animal models.
“Several lines of evidence indicate that disruption of the Isd heme acquisition system at each step (surface receptor binding, transport across the cell wall, transport across the cell membrane, or release of iron from heme) leads to decreased virulence in animal models of staphylococcal infection.”
From Metal ion acquisition in Staphylococcus aureus: overcoming nutritional immunity (2012) · cites this paper as [117]
Antibodies against IsdA and IsdB rescue animals from lethal staphylococcal challenge and inhibit abscess formation.
“Moreover, antibodies against IsdA and IsdB rescue animals from lethal staphylococcal challenge and inhibit abscess formation (39).”
From Molecular Mechanisms of Staphylococcus aureus Iron Acquisition (2011) · cites this paper as [39]

Hemoglobin binding and heme removal are carried out in vitro by distinct IsdB domains, NEAT1 and NEAT2.
“Binding of hemoglobin and removal of heme are carried out in vitro by distinct domains within IsdB called NEAT1IsdB and NEAT2IsdB, respectively (Figure 1 B ) [5, 7, 18, 28, 30, 31].”
From IsdB-dependent hemoglobin binding is required for acquisition of heme by Staphylococcus aureus (2014) · cites this paper as [28]
Inactivation of isdA, isdB, isdC, isdG, or isdI reduces bacterial proliferation in the host.
“Consistent with the importance of heme-iron acquisition to the pathogenesis of staphylococcal infections, inactivation of isdA , isdB , isdC , isdG, or isdI reduces bacterial proliferation within the host [3, 4, 21, 27 -29].”
From IsdB-dependent hemoglobin binding is required for acquisition of heme by Staphylococcus aureus (2014) · cites this paper as [28]
NEAT1 of IsdB is required and sufficient for hemoglobin binding, while NEAT2 is required and sufficient for heme binding.
“NEAT1 IsdB is required and suffi cient to bind hemoglobin, whereas NEAT2IsdB is required and suf fi cient for heme binding [7, 28].”
From IsdB-dependent hemoglobin binding is required for acquisition of heme by Staphylococcus aureus (2014) · cites this paper as [28]
NEAT1 does not contribute to heme binding by IsdB.
“Because NEAT1 does not contribute to heme binding to IsdB, this effect is not due to a reduction in heme binding [28].”
From IsdB-dependent hemoglobin binding is required for acquisition of heme by Staphylococcus aureus (2014) · cites this paper as [28]
S. aureus strains without isdB show reduced colonization in murine infection models.
“S. aureus strains lacking isdB are reduced for colonization in murine models of infection [3, 4, 27 -29].”
From IsdB-dependent hemoglobin binding is required for acquisition of heme by Staphylococcus aureus (2014) · cites this paper as [28]
Passive immunization with IsdB-specific IgG significantly improved survival of mice infected with S. aureus.
“Furthermore, passive immunization using IsdB-specific IgG significantly improved survival outcomes of mice infected with S. aureus [69].”
From A battle for iron: host sequestration and Staphylococcus aureus acquisition (2012) · cites this paper as [69]
Affinity-purified anti-IsdB antibodies given to mice before intravenous S. aureus challenge significantly reduced bacterial load and kidney abscess number.
“In a separate study affinity-purified anti-IsdB antibodies administered to mice prior to intravenous challenge with S. aureus were found to significantly reduce the bacterial load and number of abscesses formed within the kidney [69].”
From A battle for iron: host sequestration and Staphylococcus aureus acquisition (2012) · cites this paper as [69]
Anti-IsdB antisera does not promote opsonization of S. aureus in mouse blood.
“Consistent with this, anti-IsdB antisera does not promote opsonization of S. aureus in mouse blood.”
From A battle for iron: host sequestration and Staphylococcus aureus acquisition (2012) · cites this paper as [69]
Gram-positive bacteria are usually resistant to complement-mediated lysis, so clearance of S. aureus requires opsonophagocytic killing by immune cells.
“Gram-positive bacteria are typically resistant to complement mediated lysis, therefore host clearance of S. aureus requires opsonophagocytic killing by immune cells [69].”
From A battle for iron: host sequestration and Staphylococcus aureus acquisition (2012) · cites this paper as [69]
Affinity-purified rabbit antibodies against IsdB perturb hemoglobin binding.
“A potential explanation for the protective effect of anti-IsdB antibodies was provided by the observation that affinity-purified rabbit antibodies directed against IsdB perturbed hemoglobin binding [69].”
From A battle for iron: host sequestration and Staphylococcus aureus acquisition (2012) · cites this paper as [69]

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IsdB-dependent hemoglobin binding is required for acquisition of heme by Staphylococcus aureus
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Differential Function of Lip Residues in the Mechanism and Biology of an Anthrax Hemophore
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ABI domain‐containing proteins contribute to surface protein display and cell division in Staphylococcus aureus
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IsdB antibody–mediated sepsis following S. aureus surgical site infection
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Humanized Staphylococcal Enterotoxin B (SEB)–Specific Monoclonal Antibodies Protect From SEB Intoxication and Staphylococcus aureus Infections Alone or as Adjunctive Therapy With Vancomycin
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Amidase, a cell wall hydrolase, elicits protective immunity against Staphylococcus aureus and S. epidermidis
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Staphylococcus aureus in Inflammation and Pain: Update on Pathologic Mechanisms
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The near-iron transporter (NEAT) domains of the anthrax hemophore IsdX2 require a critical glutamine to extract heme from methemoglobin
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This article has been temporarily withdrawn and will be reposted within a supplement in 2017. The Press apologizes for any inconvenience caused.
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Energetics underlying hemin extraction from human hemoglobin by Staphylococcus aureus
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The iron-regulated surface proteins IsdA, IsdB, and IsdH are not required for heme iron utilization in Staphylococcus aureus
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Immunotherapy Targeting Adenosine Synthase A Decreases Severity of Staphylococcus aureus Infection in Mouse Model
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Deriving a dose and regimen for anti-glucosaminidase antibody passive-immunisation for patients with Staphylococcus aureus osteomyelitis
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Passive immunization through polyclonal antibodies against IsdE showed protective activity in an experimental S.aureus challenge model
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Authors: Kim, H. K., DeDent, A., Cheng, A. G., McAdow, M., Bagnoli, F., Missiakas, D. M., & Schneewind, O.
Affiliations: University of Chicago (US)
Corresponding author: Schneewind, Olaf
ORCID: Kim, Hwan Keun
Cheng, Alice G.
McAdow, Molly
Missiakas, Dominique M.
Schneewind, Olaf
Venue: Vaccine
Publisher: Elsevier BV
Published: 2010-08
Volume: 28
Issue: 38
Pages: 6382-6392
ISSN: 0264-410X (ISSN-L)
DOI: 10.1016/j.vaccine.2010.02.097
PMC: PMC3095377
OpenAlex: W2163053334
Cited by: 158
References: 45
Citations by year: 2026: 7 · 2025: 9 · 2024: 5 · 2023: 6 · 2022: 7 · 2021: 4 · 2020: 10 · 2019: 4 · 2018: 10 · 2017: 6 · 2016: 10 · 2015: 10 · 2014: 12 · 2013: 17 · 2012: 18
FWCI: 11.98
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Vaccine (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
Funders: National Institutes of Health (1ziahd009007-01)
National Institute of General Medical Sciences (T32GM007281, GM-07281)
National Institute of Allergy and Infectious Diseases (U54AI057153, AI52474, R01AI052474)
Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD009007)
Topics: Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
Keywords: Staphylococcus aureus, ISDA, ISDB, passive antibody transfer, subunit vaccine, abscess formation, heme binding, hemoglobin binding, intravenous challenge, bacterial virulence factors
MeSH terms: Abscess, Animals, Antibodies, Bacterial, Antigens, Bacterial, Cross Reactions, Female, Heme, Hemoglobins, Humans, Immunization, Passive, Kidney, Mice, Inbred BALB C, Mutation, Rabbits, Staphylococcal Infections, Cation Transport Proteins, Mice

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IsdA and IsdB antibodies protect mice against Staphylococcus aureus abscess formation and lethal challenge

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