The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Microtubules Facilitate Autophagosome Formation and Fusion of Autophagosomes with Endosomes
Open accessAbstract
Nutrient deprivation of eukaryotic cells provokes a variety of stress responses, including autophagy. Autophagy is carried out by autophagosomes which sequester cytosolic components and organelles for degradation after fusion with protease‐containing endosomes. To determine the role of microtubules in autophagy, we used nocodazole and vinblastine to disrupt microtubules and independently measured formation and fusion of autophagsosomes in primary rat hepatocytes. By measuring the translocation of GFP‐LC3, an autophagosomal marker, to autophagosomes and the lipidation of GFP‐LC3, we quantified the rate and magnitude of autophagosome formation. Starvation increased both the rate of autophagosome formation over the basal level and the total number of autophagosomes per cell. Maximal autophagosome formation required an intact microtubule network. Fusion of autophagosomes with endosomes, assayed by acquisition of protease‐inhibitor sensitivity as well as overlap with LysoTracker Red‐positive endosomes, required intact microtubules. Live‐cell imaging demonstrated that autophagosomes were motile structures, and their movement also required microtubules. Interestingly, vinblastine stimulated autophagosome formation more than twofold before any discernable change in the microtubule network was observed. Stimulation of autophagosome formation by vinblastine was independent of nutrients and mTOR activity but was inhibited by depletion of the A u t opha g y proteins Atg5 and Atg6, known to be required for autophagy.
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Cites
LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing.
Kabeya, Y., Mizushima, N., Ueno, T., Yamamoto, A., Kirisako, T., Noda, T., Kominami, E., Ohsumi, Y., & Yoshimori, T. (2000) · The EMBO Journal · doi:10.1093/emboj/19.21.5720 · Cited by 6,605
Tor-Mediated Induction of Autophagy via an Apg1 Protein Kinase Complex
Kamada, Y., Funakoshi, T., Shintani, T., Nagano, K., Ohsumi, M., & Ohsumi, Y. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.150.6.1507 · Cited by 1,145
Prelysosomal convergence of autophagic and endocytic pathways
Gordon, P. B., & Seglen, P. O. (1988) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(88)90556-6 · Cited by 281 · cited 1×
“In mammalian cells, the closed structure, an immature autophagosome (AVi) (20), fuses with endosomal vesicles and protease-containing late endosomal compartments (21) to become an amphisome (22).”
BackgroundKanazawa, T., Taneike, I., Akaishi, R., Yoshizawa, F., Furuya, N., Fujimura, S., & Kadowaki, M. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m306337200 · Cited by 208
Kovács, A. L., Reith, A., & Seglen, P. O. (1982) · Experimental Cell Research · doi:10.1016/0014-4827(82)90020-9 · Cited by 163
Punnonen, E.-L., & Reunanen, H. (1990) · Experimental and Molecular Pathology · doi:10.1016/0014-4800(90)90061-h · Cited by 44
Research works printed but not linked: 41
- 10.1016/j.bbrc.2003.07.023 · DOI · Paper page
- 10.1016/S1534-5807(04)00099-1 · DOI · Paper page
- 10.1242/jcs.01620 · DOI · Paper page
- Eskelinen EL, 2004, Lysosomes.
- 10.1016/S1534-5807(03)00296-X · DOI · Paper page
- 10.1074/jbc.273.7.3963 · DOI · Paper page
- 10.1074/jbc.270.5.2320 · DOI · Paper page
- Codogno P, 2004, Autophagy., 26
- 10.1016/j.bbrc.2003.07.012 · DOI · Paper page
- 10.1074/jbc.M003633200 · DOI · Paper page
- Fengsrud M, 2004, Autophagy., 11
- 10.1093/emboj/17.13.3597 · DOI · Paper page
- 10.1016/j.biocel.2004.05.009 · DOI · Paper page
- 10.1083/jcb.152.4.657 · DOI · Paper page
- 10.1091/mbc.e03-09-0704 · DOI · Paper page
- 10.1093/emboj/20.21.5971 · DOI · Paper page
- Arstila AU, 1968, Studies on cellular autophagosytosis, Am J Pathol, 53, 687
- 10.1083/jcb.110.6.1923 · DOI · Paper page
- 10.1016/0962-8924(94)90069-8 · DOI · Paper page
- 10.1016/0014-4800(88)90048-2 · DOI
- 10.1002/jcp.1041520304 · DOI · Paper page
- 10.1006/excr.1995.1402 · DOI
- Punnonen EL, 1993, Autophagic vacuoles fuse with the prelysosomal compartment in cultured rat fibroblasts, Eur J Cell Biol, 61, 54
- 10.1016/0014-4827(74)90477-7 · DOI
- Marzella L, 1980, Increased degradation in rat liver induced by vinblastine. II. Morphologic characterization, Lab Invest, 42, 18
- 10.1007/BF02890071 · DOI
- Seglen PO, 1993, Methods in Toxicology., 231
- 10.1074/jbc.274.2.1058 · DOI · Paper page
- 10.1034/j.1600-0854.2002.31204.x · DOI · Paper page
- 10.1242/jcs.01131 · DOI · Paper page
- 10.1016/j.biocel.2004.02.005 · DOI · Paper page
- 10.1073/pnas.0502857102 · DOI · Paper page
- 10.1126/science.290.5497.1717 · DOI · Paper page
- Novikoff PM, 1996, Three‐dimensional organization of rat hepatocyte cytoskeleton: relation to the asialoglycoprotein endocytosis pathway, J Cell Sci, 109, 21, 10.1242/jcs.109.1.21 · DOI
- 10.1091/mbc.12.11.3690 · DOI · Paper page
- 10.1091/mbc.e03-07-0479 · DOI
- 10.1091/mbc.11.9.3013 · DOI · Paper page
- 10.1034/j.1600-0854.2002.30704.x · DOI · Paper page
- 10.1002/jcp.1041190120 · DOI
- 10.1007/BF02473201 · DOI · Paper page
- 10.1091/mbc.02-04-0053 · DOI · Paper page
Cited by
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Acevedo Arozena, A., Adachi, H., Adams, C. M., Adams, P. D., Adeli, K., et al. (2016) · Autophagy · doi:10.1080/15548627.2015.1100356 · Cited by 5,894
Self-citationGuidelines for the use and interpretation of assays for monitoring autophagy
Klionsky, D. J., Abdalla, F. C., Abeliovich, H., Abraham, R. T., Acevedo-Arozena, A., Adeli, K., Agholme, L., Agnello, M., Agostinis, P., Aguirre-Ghiso, J. A., et al. (2012) · Autophagy · doi:10.4161/auto.19496 · Cited by 3,996
Self-citationRegulation mechanisms and signaling pathways of autophagy
He, C., & Klionsky, D. J. (2009) · Annual Review of Genetics · doi:10.1146/annurev-genet-102808-114910 · Cited by 3,609
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
Klionsky, D. J., Abdel-Aziz, A. K., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Abeliovich, H., Abildgaard, M. H., Abudu, Y. P., Acevedo-Arozena, A., et al. (2021) · Autophagy · doi:10.1080/15548627.2020.1797280 · Cited by 2,897
Self-citationGuidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
Klionsky, D. J., Abeliovich, H., Agostinis, P., Agrawal, D. K., Aliev, G., Askew, D. S., Baba, M., Baehrecke, E. H., Bahr, B. A., Ballabio, A., et al. (2008) · Autophagy · doi:10.4161/auto.5338 · Cited by 2,230
Self-citation
Metadata
- Authors
- Köchl, R., Hu, X. W., Chan, E. Y. W., & Tooze, S. A.
- Venue
- Traffic
- Publisher
- Wiley
- Published
- 2005-12-05
- Volume
- 7
- Issue
- 2
- Pages
- 129-145
- ISSN
- 1398-9219 (ISSN-L)
- 1600-0854
- OpenAlex
- W1562156325
- Cited by
- 436
- References
- 6
- FWCI
- 8.29
- Type
- journal article
- Language
- en
- Open access
- bronze
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