The bacterial SRP receptor, SecA and the ribosome use overlapping binding sites on the SecY translocon.
Open accessAbstract
Signal recognition particle (SRP)‐dependent protein targeting is a universally conserved process that delivers proteins to the bacterial cytoplasmic membrane or to the endoplasmic reticulum membrane in eukaryotes. Crucial during targeting is the transfer of the ribosome‐nascent chain complex (RNC) from SRP to the Sec translocon. In eukaryotes, this step is co‐ordinated by the SRβ subunit of the SRP receptor (SR), which probably senses a vacant translocon by direct interaction with the translocon. Bacteria lack the SRβ subunit and how they co‐ordinate RNC transfer is unknown. By site‐directed cross‐linking and fluorescence resonance energy transfer (FRET) analyses, we show that FtsY, the bacterial SRα homologue, binds to the exposed C4/C5 loops of SecY, the central component of the bacterial Sec translocon. The same loops serve also as binding sites for SecA and the ribosome. The FtsY–SecY interaction involves at least the A domain of FtsY, which attributes an important function to this so far ill‐defined domain. Binding of FtsY to SecY residues, which are also used by SecA and the ribosome, probably allows FtsY to sense an available translocon and to align the incoming SRP–RNC with the protein conducting channel. Thus, the Escherichia coli FtsY encompasses the functions of both the eukaryotic SRα and SRβ subunits in one single protein.
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A simultaneous binding of SecA and the ribosome to SecY appears unlikely, considering that both engage overlapping binding sites on SecY.
“A simultaneous binding of SecA and the ribosome to SecY appears unlikely, considering that both engage overlapping binding sites on SecY (Kuhn et al., 2011).”
From The Dynamic SecYEG Translocon (2021) · cites this paper as Kuhn et al., 2011
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- Authors
- Kuhn, P., Weiche, B., Sturm, L., Sommer, E., Drepper, F., Warscheid, B., Sourjik, V., & Koch, H.
- Affiliations
- University of Freiburg (DE)
- Heidelberg University (DE)
- Corresponding author
- Koch, Hans‐Georg
- Venue
- Traffic
- Publisher
- Wiley
- Published
- 2011-02-25
- Volume
- 12
- Issue
- 5
- Pages
- 563-578
- ISSN
- 1398-9219 (ISSN-L)
- 1600-0854
- OpenAlex
- W2088921362
- Cited by
- 76
- References
- 75
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 4 · 2020: 4 · 2019: 6 · 2018: 7 · 2017: 6 · 2016: 6 · 2015: 8 · 2014: 8 · 2013: 9 · 2012: 3
- FWCI
- 5.59
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Traffic (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft (FOR 967, (EXC 294))
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- signal recognition particle, SRP receptor, FtsY, SecA, ribosome-nascent chain complex, protein translocation, Escherichia coli
- MeSH terms
- SEC Translocation Channels, SecA Proteins, Adenosine Triphosphatases, Bacterial Proteins, Binding Sites, Escherichia coli, Humans, Models, Molecular, Protein Binding, Ribosomes, Mass Spectrometry, Protein Structure, Tertiary, Receptors, Peptide, Receptors, Cytoplasmic and Nuclear, Signal Recognition Particle, Protein Subunits, Membrane Transport Proteins, Escherichia coli Proteins, Fluorescence Resonance Energy Transfer
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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