Cited by 205Oral microbiology and periodontitis researchProbiotics and Fermented FoodsReproductive tract infections research

Anti‐inflammatory effects of Lactobacillus brevis (CD2) on periodontal disease

Riccia, DN Della, Bizzini, F, Perilli, MG, Polimeni, A, Trinchieri, V, Amicosante, G, Cifone, MG·2007·Oral Diseases

Abstract

Objectives: To analyze the anti‐inflammatory effects of Lactobacillus brevis extracts on periodontitis patients and to investigate the involved mechanisms in vitro on activated macrophages. Methods: Eight healthy subjects and 21 patients with chronic periodontitis were enrolled to analyze the effect of L. brevis‐containing lozenges on periodontitis‐associated symptoms and signs. Before and after the treatment, the patients received a complete periodontal examination. Saliva samples, collected before and after treatment, were analyzed for metalloproteinase and nitric oxide synthase (NOS) activity, immunoglobulin‐A (IgA), prostaglandin E 2 (PGE 2) and γ‐interferon (IFN‐γ) levels. Arginine deiminase (AD) and NOS activities were determined through a radiometric assay. Metalloproteinases were assayed by zymogram and Western blotting, whereas IgA, PGE 2 and IFN‐γ were assayed by enzyme‐linked imunosorbent assay tests. Results: The treatment led to the total disappearance or amelioration of all analyzed clinical parameters in all patients. This was paralleled to a significant decrease of nitrite/nitrate, PGE 2, matrix metalloproteinase, and IFN‐γ levels in saliva samples. Conclusion: Our results suggest that the anti‐inflammatory effects of L. brevis could be attributed to the presence of AD which prevented nitric oxide generation. Our findings give further insights into the knowledge of the molecular basis of periodontitis and have a potential clinical significance, giving the experimental ground for a new innovative, simple and efficacious therapeutical approach of periodontal disease.

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Crossref · 10.1111/j.1601-0825.2006.01291.x

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  • In a study in which L brevis-containing lozenges were administered to 21 patients with periodontal disease, the treatment led to complete amelioration of all clinical parameters analyzed in all patients.

    “in a study in which L brevis‐containing lozenges were administered to 21 patients with periodontal disease, the treatment led to complete amelioration of all clinical parameters analyzed in all patients.”
  • The arginine deaminase system is important beyond pH modulation and also influences the inflammatory immune response of the host to periodontal pathogens, which can damage host tissue.

    “It is now recognized that the arginine deaminase system is important in more than just pH modulation—it also influences the inflammatory immune response of the host to periodontal pathogens, which can damage host tissue.”
  • In the L brevis study, activity of the inflammatory markers prostaglandin E2, interferon-gamma, and matrix metalloproteinase were substantially decreased in treated patients.

    “In the study above, of L brevis, activity of the inflammatory markers prostaglandin E2, interferon‐gamma, and Matrix metalloproteinase were substantially decreased in treated patients.”

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Authors
Riccia, D. D., Bizzini, F., Perilli, M., Polimeni, A., Trinchieri, V, Amicosante, G., & Cifone, M.
Affiliations
  • University of L'Aquila (IT)
  • Sapienza University of Rome (IT)
Venue
Oral Diseases
Publisher
Wiley
Published
2007-05-23
Volume
13
Issue
4
Pages
376-385
ISSN
  • 1354-523X (ISSN-L)
  • 1601-0825
OpenAlex
W2157308798
Cited by
205
References
48
Citations by year
2026: 2 · 2025: 9 · 2024: 11 · 2023: 16 · 2022: 13 · 2021: 12 · 2020: 23 · 2019: 15 · 2018: 9 · 2017: 14 · 2016: 15 · 2015: 15 · 2014: 8 · 2013: 11 · 2012: 11
FWCI
3.38
Type
journal article
Language
en
Open access
closed
Locations
  • Oral Diseases (publishedVersion)
  • PubMed (publishedVersion)
  • IRIS Research product catalog (Sapienza University of Rome) (submittedVersion)
  • Archivio Istituzionale della Ricerca - Università degli Studi dell Aquila (submittedVersion)
Topics
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
Keywords
Lactobacillus brevis, periodontitis, anti-inflammatory activity, probiotics, lozenges, macrophages, arginine deiminase, nitric oxide synthase, nitric oxide, matrix metalloproteinases, prostaglandin E2, interferon-gamma, immunoglobulin A, salivary biomarkers, periodontal treatment
MeSH terms
Adult, Animals, Anti-Inflammatory Agents, Cells, Cultured, Double-Blind Method, Female, Humans, Hydrolases, Immunoglobulin A, Secretory, Interferon-gamma, Lipopolysaccharides, Male, Middle Aged, Periodontal Index, Periodontitis, Saliva, Dinoprostone, Rats, Wistar, Macrophages, Peritoneal, Nitric Oxide Synthase, Matrix Metalloproteinases, Rats, Levilactobacillus brevis

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