Inhibitory effect of 1,8-cineol (eucalyptol) on Egr-1 expression in lipopolysaccharide-stimulated THP-1 cells
Abstract
Aim To study the effects of 1,8-cineol (eucalyptol) on the expression of early growth response factor-1 (Egr-1) and NF-kappaB in the human monocyte THP-1 cell line stimulated by lipopolysaccharide (LPS).Methods The THP-1 cells were incubated with serial doses of 1,8-cineol (1, 10, and 100 mg/L, 30 min) before being stimulated with LPS (1 mg/L, 30 min). The localization of Egr-1 in the THP-1 cells was detected by immunofluorescence and a laser scanning confocal microscope. The expression of Egr-1 in the nuclei and whole cell, and NF-kappaB in the nuclei, were measured by Western blot analysis.Results When stimulated by LPS, the FITC-labeled Egr-1 was detected mainly in the nuclei. Moreover, the expression of Egr-1 in the whole cell increased markedly compared with the control cells. 1,8-Cineol pretreatment decreased the expression of Egr-1 in both the nuclei and whole cell of the LPS-stimulated THP-1 cells, and this effect was concentration-dependent, but there was no reaction on the expression of NF-kappaB in the nuclei protein in the LPS-stimulated THP-1 cells.Conclusion In a concentration-dependent manner, 1,8-Cineol reduces LPS-induced Egr-1 expression in nuclei and in whole cell of THP-1 cells, but shows no effect on NF-kappaB expression.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
1,8-Cineole may preferentially block the effect of Egr-1 by inhibiting synthesis of Egr-1 and preventing Egr-1 nuclear internalization instead of inhibiting NF-κB.
“Therefore, the authors concluded that 1,8-cineole may preferentially block the effect of Egr-1 by inhibiting the synthesis of Egr-1 and preventing Egr-1 nuclear internalization instead of NF-κB inhibition [149].”
From Antioxidant and Anti-Inflammatory Activities of Essential Oils: A Short Review (2010) · cites this paper as [149]
1,8-Cineole inhibits Egr-1 synthesis and nuclear localization induced by LPS in THP-1 cells but does not affect LPS-induced NF-κB expression in nuclei.
“Zhou et al. (2007) [149] showed that 1,8-cineole inhibited the Egr-1 synthesis and nuclear localization induced by LPS in THP-1 cells but did not affect the LPS-induced NF-κB expression in nuclei.”
From Antioxidant and Anti-Inflammatory Activities of Essential Oils: A Short Review (2010) · cites this paper as [149]
Cites
THE NF-κB AND IκB PROTEINS: New Discoveries and Insights
Baldwin, A. S. (1996) · Annual Review of Immunology · doi:10.1146/annurev.immunol.14.1.649 · Cited by 5,882 · see citing works
Schreiber, E. H., Matthias, P., Müller, M. M., & Schaffner, W. (1989) · Nucleic Acids Research · doi:10.1093/nar/17.15.6419 · Cited by 4,179 · see citing works
Establishment and characterization of a human acute monocytic leukemia cell line (THP‐1)
Tsuchiya, S., Yamabe, M., Yamaguchi, Y., Kobayashi, Y., Konno, T., & Tada, K. (1980) · International Journal of Cancer · doi:10.1002/ijc.2910260208 · Cited by 2,258 · see citing works
Juergens, U. R., Dethlefsen, U., Steinkamp, G., et al. (2003) · Respiratory Medicine · doi:10.1053/rmed.2003.1432 · Cited by 480 · see citing works
Lipopolysaccharide Induction of the Tumor Necrosis Factor-α Promoter in Human Monocytic Cells
Yao, J. J., Mackman, N., Edgington, T. S., & Fan, S.-T. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.28.17795 · Cited by 453 · see citing works
Guha, M., O'Connell, M. A., Pawliński, R., Hollis, A., McGovern, P., Yan, S.-F., Stern, D. M., & Mackman, N. (2001) · Blood · doi:10.1182/blood.v98.5.1429 · Cited by 374 · see citing works
Nagel, T., Resnick, N., Dewey, C. F., & Gimbrone, M. A. (1999) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.19.8.1825 · Cited by 357 · see citing works
Juergens, U. R., Engelen, T., Racké, K., Stöber, M., Gillissen, A., & Vetter, H. (2004) · Pulmonary Pharmacology & Therapeutics · doi:10.1016/j.pupt.2004.06.002 · Cited by 236 · see citing works
Schwachtgen, J.-L., Houston, P., Campbell, C. J., Sukhatme, V. P., & Braddock, M. (1998) · Journal of Clinical Investigation · doi:10.1172/jci1404 · Cited by 193 · see citing works
Juergens, U. R., Stöber, M., & Vetter, H. (1998) · PubMed · Cited by 167 · see citing works
Juergens, U., Stöber, M., Schmidt-Schilling, L., Kleuver, T., & Vetter, H. (1998) · PubMed · Cited by 125 · see citing works
Role of Early Growth Response-1 (Egr-1) in Interleukin-13-induced Inflammation and Remodeling
Cho, S. J., Kang, M., Homer, R., Kang, H., Zhang, X., Lee, P. J., Elias, J. A., & Lee, C. G. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m506770200 · Cited by 104 · see citing works
Xu, Z., Dziarski, R., Wang, Q., et al. (2001) · The Journal of Immunology · doi:10.4049/jimmunol.167.12.6975 · Cited by 75 · see citing works
Ye, Q., Chen, B., Tong, Z., et al. (2006) · European Respiratory Journal · doi:10.1183/09031936.06.00131505 · Cited by 70 · see citing works
Epithelial injury induces Egr-1 and Fos expression by a pathway involving protein kinase C and ERK
Dieckgraefe, B. K., & Weems, D. M. (1999) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.1999.276.2.g322 · Cited by 62 · see citing works
Mackman, N. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47202-x · Cited by 58 · see citing works
Kamimura, M., Viedt, C., Dalpke, A. H., Rosenfeld, M. E., Mackman, N., Cohen, D., Blessing, E., Preusch, M. R., Weber, C. M., Kreuzer, J., et al. (2005) · Circulation Research · doi:10.1161/01.res.0000177893.24574.13 · Cited by 58 · see citing works
Liu, L., Tsai, J. C., & Aird, W. C. (2000) · Blood · doi:10.1182/blood.v96.5.1772 · Cited by 56 · see citing works
Therapeutic modulation of transcription factor activity
Emery, J. G., Ohlstein, E. H., & Jaye, M. (2001) · Trends in Pharmacological Sciences · doi:10.1016/s0165-6147(00)01661-8 · Cited by 39 · see citing works
Effect of acetylsalicylic acid on endogenous IκB kinase activity in lung epithelial cells
Yoo, C., Lee, S., Lee, C., et al. (2001) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.2001.280.1.l3 · Cited by 33 · see citing works
The Role of MAPKs in B Cell Receptor-induced Down-regulation of Egr-1 in Immature B Lymphoma Cells
Ke, J., Gururajan, M., Kumar, A., et al. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m604671200 · Cited by 29 · see citing works
Yao, P., Tsai, M., Lin, Y., Wang, C.-H., Liao, W., Chen, J. J. W., & Yang, P. (2005) · Respiratory Research · doi:10.1186/1465-9921-6-89 · Cited by 24 · see citing works
Jeong, J., KIM, K. ‐., & Jue, D. (1997) · Immunology · doi:10.1046/j.1365-2567.1997.00335.x · Cited by 18 · see citing works
Yin, Y., Allen, P. D., Jia, L., et al. (2001) · Leukemia Research · doi:10.1016/s0145-2126(00)00147-8 · Cited by 13 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Antioxidant and Anti-Inflammatory Activities of Essential Oils: A Short Review
Miguel, M. G. (2010) · Molecules · doi:10.3390/molecules15129252 · Cited by 933 · see citing works
“Zhou et al. (2007) [149] showed that 1,8-cineole inhibited the Egr-1 synthesis and nuclear localization induced by LPS in THP-1 cells but did not affect the LPS-induced NF-κB expression in nuclei.”
Background
- Authors
- ZHOU, J.-Y., WANG, X.-F., TANG, F.-D., ZHOU, J.-Y., LU, G.-H., WANG, Y., & BIAN, R.-L.
- Affiliations
- Zhejiang University (CN)
- ORCID
- WANG, Yan
- Venue
- Acta Pharmacologica Sinica
- Published
- 2007-06
- Volume
- 28
- Issue
- 6
- Pages
- 908-912
- ISSN
- 0253-9756 (ISSN-L)
- 1671-4083
- 1745-7254
- OpenAlex
- W2146506677
- Cited by
- 35
- References
- 24
- Citations by year
- 2025: 1 · 2024: 4 · 2023: 1 · 2021: 2 · 2020: 5 · 2019: 1 · 2018: 1 · 2017: 3 · 2016: 4 · 2015: 2 · 2014: 1 · 2013: 4 · 2012: 1
- FWCI
- 5.12
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Acta Pharmacologica Sinica (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (30670930)
- Science and Technology Department of Zhejiang Province
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- 1,8-cineole, EGR1, NF-κB, lipopolysaccharide, THP-1 cells, monocytes, gene expression inhibition, anti-inflammatory activity, eucalyptus oil
- MeSH terms
- Eucalyptol, Anti-Infective Agents, Cell Line, Cell Nucleus, Cyclohexanols, DNA, Single-Stranded, Humans, Lipopolysaccharides, Monocytes, NF-kappa B, Monoterpenes, Early Growth Response Protein 1
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
