THE MEMBRANE TRANSPORT OF ASCORBIC ACID*
Abstract
A system for measuring the rate of transport of dehydroascorbate into human red blood cells shows Michaelis-Menten type kinetics with substrate inhibition at levels above 150 muM DHA. The addition of sugars impairs this transport in the diminishing hierarchy D-glucose, D-mannose, D-xylose, D-galactose, L-lyxose, D-araboascorbate, L-sorbose and 2-deoxy-D-ribose. The effect of glucose on transport of ascorbate is marked at physiological levels. Transport of DHA is accelerated by copper ion and allows dehydroascorbate to move against a concentration gradient. The evidence supports the hypotheses proposing that hyperglycemia will impair the intracellular availability of vitamin C.
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The major transport mechanism of ascorbate into the RBC is attributed to glucose transporter 1.
“The major transport mechanism of ascorbate into the RBC is attributed to glucose transporter 1 (116, 130).”
From Red Blood Cell Function and Dysfunction: Redox Regulation, Nitric Oxide Metabolism, Anemia (2017) · cites this paper as [116]
Cites
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Vitamin C: The known and the unknown and Goldilocks
Padayatty, S., & Levine, M. (2016) · Oral Diseases · doi:10.1111/odi.12446 · Cited by 753 · see citing works
Red Blood Cell Function and Dysfunction: Redox Regulation, Nitric Oxide Metabolism, Anemia
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6-Bromo-6-deoxy-l-ascorbic Acid
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- Authors
- Mann, G. V., & Newton, P.
- Affiliations
- Vanderbilt University (US)
- Corresponding author
- Mann, George V.
- Venue
- Annals of the New York Academy of Sciences
- Publisher
- Wiley
- Published
- 1975-09
- Volume
- 258
- Issue
- 1
- Pages
- 243-252
- ISSN
- 0077-8923 (ISSN-L)
- 1749-6632
- OpenAlex
- W1986734713
- Cited by
- 135
- References
- 17
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 1 · 2020: 4 · 2017: 1 · 2016: 3 · 2015: 3 · 2014: 3 · 2013: 2 · 2012: 4
- FWCI
- 0.46
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Annals of the New York Academy of Sciences (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Vitamin C and Antioxidants Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
- Keywords
- vitamin C, dehydroascorbic acid, membrane transport, human erythrocytes, Michaelis-Menten kinetics, substrate inhibition, sugar, glucose, copper ions, hyperglycemia
- MeSH terms
- Adult, Ascorbic Acid, Biological Transport, Active, Carbon Monoxide, Cell Membrane, Ceruloplasmin, Copper, Dehydroascorbic Acid, Erythrocyte Aging, Erythrocytes, Glucose, Hexoses, Humans, Hydrogen-Ion Concentration, Kinetics, Male, Pentoses
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