Nutritional Enhancement of n‐3 and n‐6 Fatty Acids in Rotifers and Artemia Nauplii by Feeding Spray‐dried Schizochytrium sp.

Abstract

A docosahexaenoic acid (DHA), 22:6(n‐3), rich strain of Schizochytrium sp. was used in a spray‐dried form to evaluate the enhancement of highly unsaturated fatty acids (HUFAs) in Artemia franciscana nauplii (Utah biotype) and the rotifer Brachionus plicatilis. This heterotrophic microalga was selected because of its high concentration of the longest chain HUFAs in the n‐3 and n‐6 series, DHA and docosapentaenoic acid (DPA), 22:5(n‐6), respectively. When 24‐h‐old Artemia nauplii were fed 400 mg/L of the algae for 24 h, the DHA content of the nauplii went from undetectable levels to 0.8% of dry weight and the omega‐3 HUFA eicosapentaenoic acid (EPA), 20:5n‐3, content went from 0.1% to 0.5% of dry weight in the nauplii. Similarly, 22:5(n‐6) increased in the nauplii from undetectable levels to 0.4% of dry weight, with a concomitant increase in arachidonic acid, (20:4n‐6), from trace to 0.3% of dry weight even though there was no arachidonic acid in the algal biomass. Similar enrichment patterns were observed in rotifers. The results suggest that spray‐dried cells of Schizochytrium sp. are effective in enriching Artemia naupli and rotifers in both n‐3 and n‐6 HUFAs. The results also suggest that Artemia nauplii and rotifers are capable of readily retroconverting 22:6(n‐3) to 20:5(n‐3) and 22:5(n‐6) to 20:4(n‐6) through the process of β‐oxidation, a well‐known process in mammals.