The Role of Cholesterol in UV Light B‐induced Apoptosis†
Open accessAbstract
Modification of major lipid raft components, such as cholesterol and ceramide, plays a role in regulation of programmed cell death under various stimuli. However, the relationship between cholesterol level modification and the activation of apoptotic signaling cascades upon UVB light has not been established. In this report, we demonstrate that upon UVB irradiation cholesterol levels in membrane rafts of skin cells increase, which leads to Fas‐receptor (Fas) aggregation in the rafts. Utilizing a continuous velocity floatation technique, we show that Fas accumulated in the lipid rafts of human melanoma M624 cells after UVB irradiation. The subsequent events of death‐inducing signaling complex formation were also detected in the lipid raft fractions. Depletion of cholesterol by methyl‐β‐cyclodextrin reduces Fas aggregation, while overloading increases. Disruption of lipid rafts also prevents Fas death domain‐associated protein (Daxx) from dissociating from Fas in the lipid rafts, which is accompanied with a reduced apoptotic, but increased nonapoptotic death of UVB‐irradiated human keratinocytes, HaCaT cells. Results indicate that cholesterol located in the plasma membrane of skin cells is required for lipid raft domain formation and activation of UVB‐induced apoptosis.
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Cholesterol presence is essential for lipid raft formation and Fas-receptor activation, and this could inhibit UV-B induced apoptosis.
“Cholesterol presence is essential for lipid raft formation and Fas-receptor activation, and this could inhibit UV-B induced apoptosis, as demonstrated by George et al. [73].”
From The Effect of Lycopene Preexposure on UV‐B‐Irradiated Human Keratinocytes (2016) · cites this paper as [73]
Depletion of cholesterol by methyl-β-cyclodextrin reduced Fas aggregation which was accompanied with a reduced apoptotic but increased nonapoptotic death of UV-B-irradiated HaCaT cells.
“Depletion of cholesterol by methyl-β-cyclodextrin reduced Fas aggregation which was accompanied with a reduced apoptotic but increased nonapoptotic death of UV-B-irradiated HaCaT cells [73].”
From The Effect of Lycopene Preexposure on UV‐B‐Irradiated Human Keratinocytes (2016) · cites this paper as [73]
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Cites
Functions of lipid rafts in biological membranes.
Brown, D. A., & London, E. (1998) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.14.1.111 · Cited by 3,027 · see citing works
Lipid rafts: heterogeneity on the high seas
PIKE, L. J. (2004) · Biochemical Journal · doi:10.1042/bj20031672 · Cited by 705 · see citing works
Aragane, Y., Kulms, D., Metze, D., Wilkes, G., Pöppelmann, B., Luger, T. A., & Schwarz, T. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.140.1.171 · Cited by 463 · see citing works
Molecular Ordering of the Initial Signaling Events of CD95
Algeciras-Schimnich, A., Shen, L., Barnhart, B. C., Murmann, A. E., Burkhardt, J. K., & Peter, M. E. (2002) · Molecular and Cellular Biology · doi:10.1128/mcb.22.1.207-220.2002 · Cited by 393 · see citing works
Ultraviolet Radiation-induced Apoptosis Is Mediated by Activation of CD-95 (Fas/APO-1)
Rehemtulla, A., Hamilton, C. A., Chinnaiyan, A. M., & Dixit, V. M. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.41.25783 · Cited by 295 · see citing works
Growth factor receptors, lipid rafts and caveolae: An evolving story
PIKE, L. J. (2005) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2005.05.005 · Cited by 269 · see citing works
CD20‐mediated apoptosis: signalling through lipid rafts
Deans, J. P., Li, H., & Polyak, M. J. (2002) · Immunology · doi:10.1046/j.1365-2567.2002.01495.x · Cited by 235 · see citing works
Ligand-independent redistribution of Fas (CD95) into lipid rafts mediates clonotypic T cell death
Muppidi, J. R., & Siegel, R. M. (2004) · Nature Immunology · doi:10.1038/ni1024 · Cited by 227 · see citing works
Li, H. Y., Appelbaum, F. R., Willman, C. L., Zager, R. A., & Banker, D. E. (2003) · Blood · doi:10.1182/blood-2002-07-2283 · Cited by 206 · see citing works
Fas/Fas Ligand Interaction Contributes to UV-Induced Apoptosis in Human Keratinocytes
Leverkus, M., Yaar, M., & Gilchrest, B. A. (1997) · Experimental Cell Research · doi:10.1006/excr.1997.3514 · Cited by 177 · see citing works
T Cell Receptor-Dependent Regulation of Lipid Rafts Controls Naive CD8+ T Cell Homeostasis
Cho, J.-H., Kim, H.-O., Surh, C. D., & Sprent, J. (2010) · Immunity · doi:10.1016/j.immuni.2009.11.014 · Cited by 154 · see citing works
The death-inducing signalling complex is recruited to lipid rafts in Fas-induced apoptosis
Scheel‐Toellner, D., Wang, K., Singh, R. P., et al. (2002) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(02)02311-2 · Cited by 130 · see citing works
Differential role of lipid rafts in the functions of CD4+ and CD8+ human T lymphocytes with aging
Larbi, A., Dupuis, G., Khalil, A., Douziech, N., Fortin, C., & Fülöp, T. (2006) · Cellular Signalling · doi:10.1016/j.cellsig.2005.08.016 · Cited by 126 · see citing works
Singleton, P. A., & Bourguignon, L. (2004) · Experimental Cell Research · doi:10.1016/j.yexcr.2003.12.025 · Cited by 108 · see citing works
Fas/Fas Ligand Interactions Are Involved in Ultraviolet-B-Induced Human Lymphocyte Apoptosis
Caricchio, R., Reap, E. A., & Cohen, P. L. (1998) · The Journal of Immunology · doi:10.4049/jimmunol.161.1.241 · Cited by 93 · see citing works
Restricted Localization of the TNF Receptor CD120a to Lipid Rafts: A Novel Role for the Death Domain
Cottin, V., Doan, J. E. S., & Riches, D. W. H. (2002) · The Journal of Immunology · doi:10.4049/jimmunol.168.8.4095 · Cited by 92 · see citing works
Cross-talk between Caveolae and Glycosylphosphatidylinositol-rich Domains
Abrami, L., Fivaz, M. L., Kobayashi, T., Kinoshita, T., Parton, R. G., & van der Goot, F. G. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m102039200 · Cited by 87 · see citing works
Tsuda, K., Furuta, N., Inaba, H., et al. (2008) · Cell Structure and Function · doi:10.1247/csf.08012 · Cited by 64 · see citing works
Yanagisawa, M., Nakamura, K., & Taga, T. (2004) · Genes to Cells · doi:10.1111/j.1365-2443.2004.00764.x · Cited by 62 · see citing works
Disruption of lipid rafts causes apoptotic cell death in HaCaT keratinocytes
Bang, B. K., Gniadecki, R., & Gajkowska, B. (2005) · Experimental Dermatology · doi:10.1111/j.0906-6705.2005.00283.x · Cited by 62 · see citing works
Lipid rafts mediate internalization of β1-integrin in migrating intestinal epithelial cells
Vassilieva, E. V., Gerner-Smidt, K., Ivanov, A. I., & Nusrat, A. (2008) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00082.2008 · Cited by 59 · see citing works
Role of skin surface lipids in UV-induced epidermal cell changes
Picardo, M., Zompetta, C., De Luca, C., Cirone, M., Faggioni, A., Nazzaro-Porro, M., Passi, S., & Prota, G. (1991) · Archives of Dermatological Research · doi:10.1007/bf00372061 · Cited by 58 · see citing works
Büschenfelde, C. M. Z., Wagner, M. M., Lutzny‐Geier, G., et al. (2009) · Leukemia · doi:10.1038/leu.2009.216 · Cited by 43 · see citing works
Ryan, L., O’Callaghan, Y. C., & O’Brien, N. M. (2004) · Journal of Biochemical and Molecular Toxicology · doi:10.1002/jbt.20007 · Cited by 40 · see citing works
Ultraviolet Radiation-induced Apoptosis Mediated by Daxx
Wu, S., Loke, H. N., & Rehemtulla, A. (2002) · Neoplasia · doi:10.1038/sj.neo.7900264 · Cited by 40 · see citing works
Self-citationIntegrin Signaling and Lipid Rafts
del Pozo, M. Á. (2004) · Cell Cycle · doi:10.4161/cc.3.6.952 · Cited by 37 · see citing works
B Cell Lipid Rafts Regulate Both Peptide-Dependent and Peptide-Independent APC-T Cell Interaction
Setterblad, N., Bécart, S., Charron, D. J., et al. (2004) · The Journal of Immunology · doi:10.4049/jimmunol.173.3.1876 · Cited by 35 · see citing works
Lipid Rafts Mediate Ultraviolet Light‐induced Fas Aggregation in M624 Melanoma Cells
Elyassaki, W., & Wu, S. (2006) · Photochemistry and Photobiology · doi:10.1562/2005-12-09-ra-748 · Cited by 35 · see citing works
Self-citationUV-B induced keratinocyte apoptosis is blocked by 2-selenium-bridged β-cyclodextrin, a GPX mimic
Mu, Y., Lv, S., Ren, X., et al. (2003) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/s1011-1344(02)00386-x · Cited by 29 · see citing works
Xiao, W., Ishdorj, G., Sun, J., et al. (2011) · Leukemia & lymphoma/Leukemia and lymphoma · doi:10.3109/10428194.2011.567317 · Cited by 17 · see citing works
Effects of Freezing and Protein Cross-Linker on Isolating Membrane Raft–Associated Proteins
George, K. S., Wu, Q., & Wu, S. (2010) · BioTechniques · doi:10.2144/000113541 · Cited by 8 · see citing works
Self-citationWu, Q., & Wu, S. (2017) · Molecular Carcinogenesis · doi:10.1002/mc.22636 · Cited by 5 · see citing works
Self-citation[Effects of UV-radiation of different spectral composition on content of sterols in rat skin].
Datsenko, Z. M., Vl, S., & Bendt, V. P. (1976) · PubMed · Cited by 4 · see citing works
Molecular Biology and Immunology for Clinicians 29
Sigal, L. H. (2004) · JCR Journal of Clinical Rheumatology · doi:10.1097/01.rhu.0000128868.23469.cf · Cited by 3 · see citing works
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The Effect of Lycopene Preexposure on UV‐B‐Irradiated Human Keratinocytes
Ascenso, A., Pedrosa, T., Pinho, S., Pinho, F., Oliveira, J. M. P. F. D., Cabral Marques, H., Oliveira, H., Simões, S., & Santos, C. (2016) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2016/8214631 · Cited by 57 · see citing works
- Authors
- George, K. S., Elyassaki, W., Wu, Q., & Wu, S.
- Affiliations
- Ohio University (US)
- Battelle (US)
- Corresponding author
- Wu, Shiyong
- ORCID
- Venue
- Photochemistry and Photobiology
- Publisher
- Wiley
- Published
- 2011-12-16
- Volume
- 88
- Issue
- 5
- Pages
- 1191-1197
- ISSN
- 0031-8655 (ISSN-L)
- 1751-1097
- PMC
- PMC3297721
- OpenAlex
- W1501426363
- Cited by
- 23
- References
- 34
- Citations by year
- 2025: 2 · 2023: 1 · 2022: 1 · 2021: 3 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 3 · 2015: 1 · 2014: 2 · 2013: 3 · 2012: 3
- FWCI
- 1.04
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Photochemistry and Photobiology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Cancer Institute (R01CA086928)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Caveolin-1 and cellular processes
- Keywords
- cholesterol, UVB-induced apoptosis, lipid rafts, death-inducing signaling complex, DAXX, methyl-β-cyclodextrin, ceramide, melanoma cells, keratinocytes, non-apoptotic cell death, skin cells
- MeSH terms
- Cholesterol, Humans, Melanoma, Nuclear Proteins, Protein Binding, Ultraviolet Rays, Signal Transduction, Keratinocytes, Gene Expression, Apoptosis, Molecular Chaperones, fas Receptor, Membrane Microdomains, Cell Line, Tumor, beta-Cyclodextrins, Adaptor Proteins, Signal Transducing, Co-Repressor Proteins
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