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Cited by 160Bacterial Genetics and BiotechnologyGene Regulatory Network AnalysisMicrobial Metabolic Engineering and Bioproduction

The application of Tet repressor in prokaryotic gene regulation and expression

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Bertram, Ralph, Hillen, Wolfgang·2008·Microbial Biotechnology
DOI 10.1111/j.1751-7915.2007.00001.xPMC PMC3864427
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Abstract

Summary: Inducible gene expression based upon Tet repressor ( tet regulation) is a broadly applied tool in molecular genetics. In its original environment, Tet repressor (TetR) negatively controls tetracycline (tc) resistance in bacteria. In the presence of tc, TetR is induced and detaches from its cognate DNA sequence tetO , so that a tc antiporter protein is expressed. In this article, we provide a comprehensive overview about tet regulation in bacteria and illustrate the parameters of different regulatory architectures. While some of these set‐ups rely on natural tet ‐control regions like those found on transposon Tn 10 , highly efficient variations of this system have recently been adapted to different Gram‐negative and Gram‐positive bacteria. Novel tet ‐controllable artificial or hybrid promoters were employed for target gene expression. They are controlled by regulators expressed at different levels either in a constitutive or in an autoregulated manner. The resulting tet systems have been used for various purposes. We discuss integrative elements vested with tc‐sensitive promoters, as well as tet regulation in Gram‐negative and Gram‐positive bacteria for analytical purposes and for protein overproduction. Also the use of TetR as an in vivo biosensor for tetracyclines or as a regulatory device in synthetic biology constructs is outlined. Technical specifications underlying different regulatory set‐ups are highlighted, and finally recent developments concerning variations of TetR are presented, which may expand the use of prokaryotic tet systems in the future.

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  • The Tet-Off and Tet-On systems are based on the Tet repressor protein (TetR) and tet operator (tetO) DNA elements that control the Tn10-encoded tetracycline resistance operon of Escherichia coli.

    “The Tet-Off and Tet-On systems are based on the Tet repressor protein (TetR) and tet operator (tetO) DNA elements that control the Tn10-encoded tetracycline resistance operon of Escherichia coli.”

    From Tet-On Systems For Doxycycline-inducible Gene Expression (2016) · cites this paper as [7]

    The ‘Origin of tet regulation’ section (page unavailable) describes TetR binding tetO to regulate the Tn10 tetracycline-resistance genes, supporting the claim about the underlying components. Our copy does not establish that the specifically named Tet-Off and Tet-On systems are based on those components. (LLM, conf=0.82)

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Authors
Bertram, R., & Hillen, W.
Affiliations
Friedrich-Alexander-Universität Erlangen-Nürnberg (DE)
Corresponding author
Bertram, Ralph
ORCID
Bertram, Ralph
Venue
Microbial Biotechnology
Publisher
Wiley
Published
2007-08-24
Volume
1
Issue
1
Pages
2-16
ISSN
1751-7915 (ISSN-L)
DOI
10.1111/j.1751-7915.2007.00001.x
PMC
PMC3864427
OpenAlex
W2035018687
Cited by
160
References
1
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FWCI
0.38
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Microbial Biotechnology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Deutsche Forschungsgemeinschaft (SFB 473)
  • Volkswagen Foundation
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords
Tet repressor, tetracycline regulation, tetracycline resistance, inducible promoters, autoregulation, protein overexpression, synthetic biology, prokaryotic gene regulation, Gram-negative bacteria, Gram-positive bacteria
MeSH terms
Bacteria, Bacterial Proteins, Base Sequence, Molecular Sequence Data, Repressor Proteins, Tetracycline, Gene Expression Regulation, Bacterial

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Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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